breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | REL606 | 3,492,082 | 0 | T | G | 47.4% | 30.7 / 27.6 | 38 | V490G (GTG→GGG) | glpD | sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding |
* | REL606 | 2,197,867 | 0 | T | G | 42.4% | 47.2 / 13.0 | 33 | A47A (GCA→GCC) | cirA | ferric iron‑catecholate outer membrane transporter |
* | REL606 | 2,231,892 | 0 | A | C | 38.2% | 52.1 / 10.2 | 34 | T25P (ACC→CCC) | yejH | predicted ATP‑dependet helicase |
* | REL606 | 273,581 | 0 | T | G | 37.5% | 80.9 / 17.0 | 56 | G136G (GGT→GGG) | yagU | conserved inner membrane protein |
* | REL606 | 2,193,166 | 0 | G | C | 36.8% | 27.5 / 11.7 | 19 | A347G (GCG→GGG) | yeiB | conserved inner membrane protein |
* | REL606 | 154,497 | 0 | T | G | 35.2% | 97.8 / 21.2 | 54 | A191A (GCA→GCC) | yadL | predicted fimbrial‑like adhesin protein |
* | REL606 | 3,733,719 | 0 | C | G | 32.3% | 50.7 / 17.5 | 31 | G63G (GGC→GGG) | gumD | ADP‑L‑glycero‑D‑mannoheptose‑6‑epimerase, NAD(P)‑binding |
* | REL606 | 1,795,650 | 0 | A | C | 31.4% | 116.3 / 11.9 | 51 | V39G (GTG→GGG) | celF | cryptic phospho‑beta‑glucosidase, NAD(P)‑binding |
* | REL606 | 2,296,329 | 0 | A | C | 30.2% | 111.0 / 17.1 | 53 | intergenic (‑149/‑66) | inaA/yfaH | hypothetical protein/hypothetical protein |
* | REL606 | 1,747,903 | 0 | G | T | 30.0% | 152.2 / 57.2 | 70 | A8S (GCA→TCA) | ydiL | hypothetical protein |
* | REL606 | 3,691,844 | 0 | T | G | 29.8% | 93.4 / 12.3 | 47 | N226T (AAC→ACC) | yiaY | predicted Fe‑containing alcohol dehydrogenase |
Marginal new junction evidence (lowest skew 10 of 96 shown) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | REL606 | 2040618 = | 31 (0.650) | 10 (0.230) | 9/134 | 3.3 | 20.5% | coding (766/1113 nt) | vioA | VioA, involved in dTDP‑N‑acetylviosamine synthesis |
? | REL606 | 2040858 = | 50 (1.170) | coding (526/1113 nt) | vioA | VioA, involved in dTDP‑N‑acetylviosamine synthesis | |||||
* | ? | REL606 | = 2037392 | 28 (0.590) | 9 (0.210) | 7/136 | 4.0 | 32.5% | coding (739/1176 nt) | wzy | O antigen polymerase (Wzy) |
? | REL606 | = 2037686 | 12 (0.280) | coding (445/1176 nt) | wzy | O antigen polymerase (Wzy) | |||||
* | ? | REL606 | = 2039480 | 32 (0.670) | 6 (0.140) | 6/134 | 4.3 | 20.5% | coding (215/747 nt) | wbbA | putative glycosyltransferase WbbA |
? | REL606 | = 2039680 | 18 (0.420) | coding (15/747 nt) | wbbA | putative glycosyltransferase WbbA | |||||
* | ? | REL606 | = 2037854 | 32 (0.670) | 6 (0.140) | 6/132 | 4.3 | 18.7% | coding (277/1176 nt) | wzy | O antigen polymerase (Wzy) |
? | REL606 | = 2037988 | 24 (0.570) | coding (143/1176 nt) | wzy | O antigen polymerase (Wzy) | |||||
* | ? | REL606 | 2041277 = | 33 (0.690) | 7 (0.160) | 6/136 | 4.4 | 14.3% | coding (107/1113 nt) | vioA | VioA, involved in dTDP‑N‑acetylviosamine synthesis |
? | REL606 | 2041470 = | 54 (1.250) | coding (1351/1428 nt) | wzx | O antigen translocase (Wzx) | |||||
* | ? | REL606 | = 1506341 | 20 (0.420) | 8 (0.180) | 6/140 | 4.5 | 30.0% | intergenic (+203/‑37) | ECB_01417/ydcC | hypothetical protein/hypothetical protein |
? | REL606 | = 1506614 | NA (NA) | coding (237/792 nt) | ydcC | hypothetical protein | |||||
* | ? | REL606 | = 719226 | 68 (1.420) | 8 (0.180) | 6/140 | 4.5 | 10.7% | coding (242/762 nt) | ybfD | hypothetical protein |
? | REL606 | = 3555024 | 70 (1.570) | intergenic (+204/‑42) | yrhC/yhhI | b4552; predicted protein fragment/predicted transposase | |||||
* | ? | REL606 | = 718943 | NA (NA) | 8 (0.180) | 6/140 | 4.5 | 11.2% | intergenic (+89/‑42) | yhhI/ybfD | predicted transposase; b3484_1/hypothetical protein |
? | REL606 | = 719226 | 68 (1.420) | coding (242/762 nt) | ybfD | hypothetical protein | |||||
* | ? | REL606 | = 242771 | NA (NA) | 8 (0.180) | 6/140 | 4.5 | 11.2% | intergenic (+389/‑42) | ECB_00212/yhhI | hypothetical protein/predicted transposase |
? | REL606 | = 719226 | 68 (1.420) | coding (242/762 nt) | ybfD | hypothetical protein | |||||
* | ? | REL606 | 2039235 = | 26 (0.540) | 6 (0.130) | 6/144 | 4.6 | 16.4% | coding (460/747 nt) | wbbA | putative glycosyltransferase WbbA |
? | REL606 | 2039555 = | 36 (0.790) | coding (140/747 nt) | wbbA | putative glycosyltransferase WbbA |