Predicted mutation
evidence seq id position mutation annotation gene description
MC JC REL606 3,147,765 Δ27 bp [ygjF] [ygjF]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ REL606 3147765 3147791 27 33 [0] [0] 33 [ygjF] [ygjF]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 = 31477640 (0.000)30 (0.800) 25/142 0.5 100% intergenic (+59/+20) rpoD/ygjF RNA polymerase sigma factor/G/U mismatch‑specific DNA glycosylase
?REL606 3147792 = 0 (0.000)coding (499/507 nt) ygjF G/U mismatch‑specific DNA glycosylase

ATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACGCCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTCAACCAGTTTCTCCAGTGAAACGCGACTTAAACCG  >  REL606/3147718‑3147865
                                                                          |                                                                         
ttcctggacgattaatcggtaggccggatcaggcgttacgccgcacccggcactaggccctctgcacaaacgccCa                                                                          <  1:129757‑M2/2‑1 (MQ=255)
      gacgattaatcggtaggccggatcaggcgttacgccgcacccggcactaggccctctgcacaaacgccCACGCACt                                                                    <  2:880016‑M2/8‑1 (MQ=255)
        cgattaatcggtaggccggatcaggcgttacgccgcacccggcactaggccctctgcacaaacgccCACGCACTAc                                                                  >  1:1131661‑M2/67‑76 (MQ=255)
        cgattaatcggtaggccggatcaggcgttacgccgcacccggcactaggccctctgcacaaacgccCACGCACTAc                                                                  >  1:19584‑M2/67‑76 (MQ=255)
             aatcggtaggccggatcaggcgttacgccgcacccggcactaggccctctgcacaaacgccCACGCACTACCAgcg                                                             <  1:126842‑M2/15‑1 (MQ=255)
              atcggtaggccggatcaggcgttacgccgcacccggcactaggcccgctgcacaaacgccCACGCACTACCCgcgc                                                            >  1:979284‑M2/61‑76 (MQ=255)
                    aggccggatcaggcgttacgccgcacccggcactaggccctctgcacaaacgccCACGCACTACCAGCGCCTGGTc                                                      <  1:597279‑M2/22‑1 (MQ=255)
                       ccggatcaggcgttacgccgcacccggcactaggccctctgcacaaacgccCACGCACTACCAGCGCCTGGTCCAg                                                   >  1:325887‑M2/52‑76 (MQ=255)
                        cggatcaggcgttacgccgcacccggcactaggccctctgcacaaacgccCACGCACTACCAGCGCCTGGTCCAGc                                                  >  1:154970‑M2/51‑76 (MQ=255)
                           atcaggcgttacgccgcacccggcactaggccctctgcacaaacgccCACGCACTACCAGCGCCTGGTCCAGCTcg                                               <  1:221518‑M2/29‑1 (MQ=255)
                           atcaggcgttacgccgcacccggcactaggccctctgcacaaacgccCACGCACTACCAGCGCCTGGTCCAGCTcg                                               <  1:335388‑M2/29‑1 (MQ=255)
                           atcaggcgttacgccgcacccggcactaggccctctgcacaaacgccCACGCACTACCAGCGCCTGGTCCAGCTcg                                               >  1:521037‑M2/48‑76 (MQ=255)
                            tcaggcgttacgccgcacccggcactaggccctctgcacaaagaccGACGCACTAGCAGCGGCTGGTCCagctagt                                              >  2:77451‑M2/47‑73 (MQ=255)
                             caggcgttacgccgcacccggcactaggccctctgcacaaacgccCACGCACTACCAGCGCCTGGTCCAGCTcgcg                                             >  1:1124021‑M2/46‑76 (MQ=255)
                             caggcgttacgccgcacccggcactaggccctctgcacaaacgccCACGCACTACCAGCGCCTGGTCCAGCTcgcg                                             <  1:358322‑M2/31‑1 (MQ=255)
                              aggcgttacgccgcacccggcactaggccctctgcacaaacgccCACGCACTACCAGCGCCTGGTCCAGCTCGCGa                                            >  2:1082587‑M2/45‑76 (MQ=255)
                                  gttacgccgcccccggcactagcgcctctgcacaaacgccCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACg                                        <  1:549‑M2/36‑1 (MQ=255)
                                  gttacgccgcacccggcactaggccctctgcacaaacgccCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACg                                        <  1:472022‑M2/36‑1 (MQ=255)
                                    tacgccgcacccggcactaggccctctgcacaaacgccCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCt                                      <  1:898864‑M2/38‑1 (MQ=255)
                                    tacgccgcacccggcactaggccctctgcacaaacgccCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCt                                      >  2:493570‑M2/39‑76 (MQ=255)
                                    tacgccgcacccggcactaggccctctgcacaaacgccCACGCACTACCAGCGCCTGGTCCAGCCCCCGATACGCt                                      >  1:56854‑M2/39‑76 (MQ=255)
                                     acgccgcacccggcactaggccctctgcacaaacgccCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCtt                                     <  1:1159835‑M2/39‑1 (MQ=255)
                                      cgccgcacccggcactaggccctctgcacaaactccCCCGCACTATCAGCGCCTGCTCCAGCGCGCGGTATGCTTc                                    >  2:578069‑M2/37‑76 (MQ=255)
                                       gccgcacccggcactaggccctctgcacaaacgccCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTCa                                   <  1:452974‑M2/41‑1 (MQ=255)
                                        ccgcacccggcactaggccctctgcacaaacgccCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTCaa                                  >  2:1039886‑M2/35‑76 (MQ=255)
                                                  cactaggccctctgcacaaacgccCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTCAACCAGTTtctc                        <  2:1188778‑M2/52‑1 (MQ=255)
                                                     taggccctctgcacaaacgccCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTCAACCAGTTTCTCCAg                     <  2:459786‑M2/55‑1 (MQ=255)
                                                           ctctgcacaaacgccCACGCACTACCAGCGCCTGGTCCAGCTCACGATACGCTTCAACCAGTTTCTCCAGTGAAAc               >  1:101773‑M2/16‑76 (MQ=255)
                                                                  caaacgccCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTCAACCAGTTTCTCCAGTGAAACGCGACtt        >  2:376459‑M2/9‑76 (MQ=255)
                                                                    aacgccCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTCAACCAGTTTCTCCAGTGAAACGCGACTTaa      >  2:730630‑M2/7‑76 (MQ=255)
                                                                      cgccCCCGCCCTACCAGCGCCTGGTCCAGCTCGCGATACGCTTCAACCAGTTTCTCCAGTGAAACGCGACTTAAAc    <  2:101773‑M2/72‑1 (MQ=255)
                                                                       gccCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTCAACCAGTTTCTCCAGTGAAACGCGACTTAAAcc   >  1:1244509‑M2/4‑76 (MQ=255)
                                                                        ccCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTCAACCAGTTTCTCCAGTGAAACGCGACTTAAACCg  >  1:652491‑M2/3‑76 (MQ=255)
                                                                          |                                                                         
ATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACGCCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTCAACCAGTTTCTCCAGTGAAACGCGACTTAAACCG  >  REL606/3147718‑3147865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.