breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 14,116 A→G 6.3% intergenic (+37/‑52) dnaK → / → dnaJ chaperone Hsp70, with co‑chaperone DnaJ/chaperone Hsp40, DnaK co‑chaperone
RA 14,119 G→A 6.1% intergenic (+40/‑49) dnaK → / → dnaJ chaperone Hsp70, with co‑chaperone DnaJ/chaperone Hsp40, DnaK co‑chaperone
RA 14,124 T→A 6.2% intergenic (+45/‑44) dnaK → / → dnaJ chaperone Hsp70, with co‑chaperone DnaJ/chaperone Hsp40, DnaK co‑chaperone
RA 14,129 T→C 6.5% intergenic (+50/‑39) dnaK → / → dnaJ chaperone Hsp70, with co‑chaperone DnaJ/chaperone Hsp40, DnaK co‑chaperone
RA 14,132 C→T 6.4% intergenic (+53/‑36) dnaK → / → dnaJ chaperone Hsp70, with co‑chaperone DnaJ/chaperone Hsp40, DnaK co‑chaperone
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 357,692 C→A 5.1% intergenic (+238/+99) codA → / ← cynR cytosine/isoguanine deaminase/transcriptional activator of cyn operon; autorepressor
RA 526,792 A→C 24.1% S1178R (AGC→CGC)  rhsD → Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
RA 526,796 C→G 24.0% A1179G (GCG→GGG)  rhsD → Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
RA 526,800 A→G 23.4% E1180E (GAA→GAG rhsD → Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
RA 659,204 C→T 5.1% intergenic (+54/+47) tatE → / ← lipA TatABCE protein translocation system subunit/lipoate synthase
RA 659,205 A→T 5.4% intergenic (+55/+46) tatE → / ← lipA TatABCE protein translocation system subunit/lipoate synthase
RA 659,206 A→T 5.0% intergenic (+56/+45) tatE → / ← lipA TatABCE protein translocation system subunit/lipoate synthase
RA 659,207 A→G 5.2% intergenic (+57/+44) tatE → / ← lipA TatABCE protein translocation system subunit/lipoate synthase
RA 712,875:1 +C 100% intergenic (‑73/‑112) ybfF ← / → seqA acyl‑CoA esterase/negative modulator of initiation of replication
RA 747,895 C→G 5.3% intergenic (‑126/+26) abrB ← / ← ybgO regulator of aidB expression; inner membrane protein/putative fimbrial protein
RA 747,897 G→C 5.5% intergenic (‑128/+24) abrB ← / ← ybgO regulator of aidB expression; inner membrane protein/putative fimbrial protein
RA 747,900 G→C 5.5% intergenic (‑131/+21) abrB ← / ← ybgO regulator of aidB expression; inner membrane protein/putative fimbrial protein
RA 747,902 C→G 5.3% intergenic (‑133/+19) abrB ← / ← ybgO regulator of aidB expression; inner membrane protein/putative fimbrial protein
RA 1,097,158 C→T 9.8% intergenic (+329/+407) ycdU → / ← serX putative inner membrane protein/tRNA‑Ser
RA 1,141,153 A→G 5.3% intergenic (+167/+29) flgL → / ← rne flagellar hook‑filament junction protein/endoribonuclease; RNA‑binding protein;RNA degradosome binding protein
RA 1,529,175 T→C 100% pseudogene (1274/2037 nt) rhsE → pseudogene, Rhs family
RA 1,529,180 T→C 100% pseudogene (1279/2037 nt) rhsE → pseudogene, Rhs family
RA 1,529,185 C→T 100% pseudogene (1284/2037 nt) rhsE → pseudogene, Rhs family
RA 1,529,203 C→A 100% pseudogene (1302/2037 nt) rhsE → pseudogene, Rhs family
RA 1,529,218 C→T 100% pseudogene (1317/2037 nt) rhsE → pseudogene, Rhs family
RA 1,666,470 C→A 5.0% L386I (CTA→ATA)  clcB → H(+)/Cl(‑) exchange transporter
RA 1,873,691 T→C 5.3% S40P (TCA→CCA)  yeaK → aminoacyl‑tRNA editing domain protein
RA 1,873,694 G→C 5.3% E41Q (GAA→CAA) ‡ yeaK → aminoacyl‑tRNA editing domain protein
RA 1,873,695 A→G 5.6% E41G (GAA→GGA) ‡ yeaK → aminoacyl‑tRNA editing domain protein
RA 1,873,700 C→T 5.2% R43C (CGT→TGT) ‡ yeaK → aminoacyl‑tRNA editing domain protein
RA 1,873,701 G→C 5.4% R43P (CGT→CCT) ‡ yeaK → aminoacyl‑tRNA editing domain protein
RA 1,873,704 G→A 5.2% G44D (GGC→GAC)  yeaK → aminoacyl‑tRNA editing domain protein
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,317,011 A→T 5.2% intergenic (+184/+16) rcsB → / ← rcsC response regulator in two‑component regulatory system with RcsC and YojN/hybrid sensory kinase in two‑component regulatory system with RcsB and YojN
RA 2,317,012 A→T 5.2% intergenic (+185/+15) rcsB → / ← rcsC response regulator in two‑component regulatory system with RcsC and YojN/hybrid sensory kinase in two‑component regulatory system with RcsB and YojN
RA 2,317,018 T→G 5.2% intergenic (+191/+9) rcsB → / ← rcsC response regulator in two‑component regulatory system with RcsC and YojN/hybrid sensory kinase in two‑component regulatory system with RcsB and YojN
RA 2,894,856 C→A 5.4% intergenic (+85/+63) ygcP → / ← ygcQ putative antiterminator regulatory protein/putative flavoprotein
RA 3,560,455:1 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,608,695 C→G 8.4% intergenic (+46/+56) zntA → / ← tusA zinc, cobalt and lead efflux system/mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase
RA 3,700,416 T→C 5.2% intergenic (+215/‑147) rdlD → / → yhjV sRNA antisense regulator affects LdrD translation; proposed addiction module in LDR‑D repeat, with toxic peptide LdrD/putative transporter
RA 3,700,418 G→A 5.0% intergenic (+217/‑145) rdlD → / → yhjV sRNA antisense regulator affects LdrD translation; proposed addiction module in LDR‑D repeat, with toxic peptide LdrD/putative transporter
RA 3,700,419 T→C 5.0% intergenic (+218/‑144) rdlD → / → yhjV sRNA antisense regulator affects LdrD translation; proposed addiction module in LDR‑D repeat, with toxic peptide LdrD/putative transporter
RA 3,700,421 G→A 5.4% intergenic (+220/‑142) rdlD → / → yhjV sRNA antisense regulator affects LdrD translation; proposed addiction module in LDR‑D repeat, with toxic peptide LdrD/putative transporter
RA 3,918,291 C→T 5.7% intergenic (‑26/+25) atpG ← / ← atpA F1 sector of membrane‑bound ATP synthase, gamma subunit/F1 sector of membrane‑bound ATP synthase, alpha subunit
RA 3,918,296 C→T 6.4% intergenic (‑31/+20) atpG ← / ← atpA F1 sector of membrane‑bound ATP synthase, gamma subunit/F1 sector of membrane‑bound ATP synthase, alpha subunit
RA 3,918,299 A→G 6.3% intergenic (‑34/+17) atpG ← / ← atpA F1 sector of membrane‑bound ATP synthase, gamma subunit/F1 sector of membrane‑bound ATP synthase, alpha subunit
RA 3,918,304 A→G 6.2% intergenic (‑39/+12) atpG ← / ← atpA F1 sector of membrane‑bound ATP synthase, gamma subunit/F1 sector of membrane‑bound ATP synthase, alpha subunit
RA 4,296,190 A→G 18.3% intergenic (+396/+246) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,191 A→C 16.7% intergenic (+397/+245) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,268 T→C 32.0% intergenic (+474/+168) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,286 C→T 21.0% intergenic (+492/+150) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,303 A→G 8.5% intergenic (+509/+133) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,304 A→C 5.8% intergenic (+510/+132) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380:1 +C 94.8% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380:2 +G 94.8% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,593,430 G→T 5.2% V431V (GTC→GTA) ‡ lgoT ← putative L‑galactonate:H+ symporter
RA 4,593,431 A→G 5.2% V431A (GTC→GCC) ‡ lgoT ← putative L‑galactonate:H+ symporter
RA 4,593,434 C→T 5.2% C430Y (TGC→TAC) ‡ lgoT ← putative L‑galactonate:H+ symporter
RA 4,593,435 A→C 5.1% C430G (TGC→GGC) ‡ lgoT ← putative L‑galactonate:H+ symporter

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423733–3424234 3424553–3424238 5–821 90 [89] [89] 90 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =90 (0.610)29 (0.210) 28/532 NT 23.8% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 101 (0.730)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780591 (0.620)25 (0.180) 25/532 NT 21.1% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 101 (0.730)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)161 (1.110) 129/556 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein