breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 526,792 A→C 16.7% S1178R (AGC→CGC)  rhsD → Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
RA 526,796 C→G 14.9% A1179G (GCG→GGG)  rhsD → Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
RA 526,800 A→G 14.3% E1180E (GAA→GAG rhsD → Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
RA 551,372 C→A 5.1% intergenic (+40/+155) ybcF → / ← purK putative carbonate kinase/N5‑carboxyaminoimidazole ribonucleotide synthase
JC 712,853 +GTGCCACAGG 100% intergenic (‑51/‑134) ybfF ← / → seqA acyl‑CoA esterase/negative modulator of initiation of replication
RA 1,117,876 C→G 5.4% intergenic (+17/+25) yceA → / ← yceI putative rhodanese‑related sulfurtransferase/periplasmic high pH‑inducible lipid‑binding protein
RA 1,117,877 G→C 5.4% intergenic (+18/+24) yceA → / ← yceI putative rhodanese‑related sulfurtransferase/periplasmic high pH‑inducible lipid‑binding protein
RA 1,117,878 T→A 5.3% intergenic (+19/+23) yceA → / ← yceI putative rhodanese‑related sulfurtransferase/periplasmic high pH‑inducible lipid‑binding protein
RA 1,117,879 G→C 5.3% intergenic (+20/+22) yceA → / ← yceI putative rhodanese‑related sulfurtransferase/periplasmic high pH‑inducible lipid‑binding protein
RA 1,169,380 T→C 5.8% intergenic (+50/+32) bhsA → / ← ldtC biofilm, cell surface and signaling protein/L,D‑transpeptidase linking Lpp to murein
RA 1,169,382 C→G 5.2% intergenic (+52/+30) bhsA → / ← ldtC biofilm, cell surface and signaling protein/L,D‑transpeptidase linking Lpp to murein
RA 1,169,384 G→A 5.3% intergenic (+54/+28) bhsA → / ← ldtC biofilm, cell surface and signaling protein/L,D‑transpeptidase linking Lpp to murein
RA 1,529,175 T→C 100% pseudogene (1274/2037 nt) rhsE → pseudogene, Rhs family
RA 1,529,180 T→C 100% pseudogene (1279/2037 nt) rhsE → pseudogene, Rhs family
RA 1,529,185 C→T 100% pseudogene (1284/2037 nt) rhsE → pseudogene, Rhs family
RA 1,529,203 C→A 100% pseudogene (1302/2037 nt) rhsE → pseudogene, Rhs family
RA 1,529,218 C→T 100% pseudogene (1317/2037 nt) rhsE → pseudogene, Rhs family
RA 1,950,306 C→G 5.0% G73R (GGC→CGC)  aspS ← aspartyl‑tRNA synthetase
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,630,921 A→T 5.1% intergenic (+56/+37) yfgI → / ← guaA nalidixic acid resistance protein, putative periplasmic protein/GMP synthetase (glutamine aminotransferase)
RA 2,911,007 G→A 5.1% R29C (CGT→TGT)  mazF ← mRNA interferase toxin, antitoxin is MazE
RA 2,911,008 A→T 5.2% H28Q (CAT→CAA) ‡ mazF ← mRNA interferase toxin, antitoxin is MazE
RA 2,911,009 T→C 5.2% H28R (CAT→CGT) ‡ mazF ← mRNA interferase toxin, antitoxin is MazE
RA 3,233,708 A→G 5.3% intergenic (+25/+20) fadH → / ← higA 2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked/antitoxinof the HigB‑HigA toxin‑antitoxin system
RA 3,233,709 A→T 5.0% intergenic (+26/+19) fadH → / ← higA 2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked/antitoxinof the HigB‑HigA toxin‑antitoxin system
RA 3,233,710 C→T 5.0% intergenic (+27/+18) fadH → / ← higA 2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked/antitoxinof the HigB‑HigA toxin‑antitoxin system
RA 3,477,345 A→T 5.3% A25A (GCT→GCA fkpA ← FKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)
RA 3,560,455:1 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,831,757 C→T 6.3% A434V (GCC→GTC)  yicH → putative inner membrane‑anchored periplasmic AsmA family protein
RA 3,831,761 C→G 6.3% A435A (GCC→GCG yicH → putative inner membrane‑anchored periplasmic AsmA family protein
RA 3,831,764 C→G 6.4% A436A (GCC→GCG yicH → putative inner membrane‑anchored periplasmic AsmA family protein
RA 3,831,768 A→G 6.2% T438A (ACA→GCA)  yicH → putative inner membrane‑anchored periplasmic AsmA family protein
RA 3,875,522 C→G 6.8% L29V (CTG→GTG)  yidX → putative lipoprotein
RA 4,296,190 A→G 29.9% intergenic (+396/+246) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,191 A→C 30.3% intergenic (+397/+245) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,268 T→C 34.8% intergenic (+474/+168) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,286 C→T 22.5% intergenic (+492/+150) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,303 A→G 12.4% intergenic (+509/+133) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,304 A→C 10.8% intergenic (+510/+132) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,472,236 T→G 12.3% T54P (ACC→CCC)  pyrB ← aspartate carbamoyltransferase, catalytic subunit

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423742–3424503 3424503 1–762 90 [89] [89] 90 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 271133NA (NA)4 (0.030) 4/554 NT NA noncoding (593/1221 nt) IS30 repeat region
?NC_000913 = 271141 NA (NA)noncoding (601/1221 nt) IS30 repeat region
* ? NC_000913 274347 =NA (NA)8 (0.050) 6/550 NT NA noncoding (803/1195 nt) IS5 repeat region
?NC_000913 274410 = NA (NA)noncoding (740/1195 nt) IS5 repeat region
* ? NC_000913 1096961 =140 (0.920)29 (0.270) 10/404 NT 23.9% intergenic (+132/+604) ycdU/serX putative inner membrane protein/tRNA‑Ser
?NC_000913 = 1097503 84 (0.770)intergenic (+674/+62) ycdU/serX putative inner membrane protein/tRNA‑Ser
* ? NC_000913 1207790 =15 (0.100)126 (0.880) 112/532 NT 88.4% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 19 (0.130)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780514 (0.090)122 (0.850) 105/532 NT 88.3% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 19 (0.130)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)177 (1.180) 140/556 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 3620350NA (NA)9 (0.060) 8/552 NT 100% coding (1159/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
?NC_000913 = 3620425 0 (0.000)coding (1234/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor