breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 24 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,993,9160AC60.0% 16.5 / 27.2 35intergenic (+205/‑341)kefB‑III/PP_mr64glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
*NC_0029471,187,4610GT56.2% 9.4 / 18.7 16P37P (CCG→CCTtadAtRNA adenosine(34) deaminase TadA
*NC_0029475,514,9020TG55.6% ‑6.0 / 12.5 31L35R (CTG→CGG) urtBurea ABC transporter permease
*NC_0029475,939,8600GT53.8% 2.7 / 12.3 13intergenic (+10/‑125)PP_mr61/fau6S ncRNA/5‑formyltetrahydrofolate cyclo‑ligase
*NC_0029473,425,6370AC53.3% 5.0 / 16.1 15intergenic (‑35/+74)PP_3035/PP_5554hypothetical protein/hypothetical protein
*NC_0029472,092,4430GA48.3% 3.0 / 33.3 29intergenic (+264/+654)PP_1865/aroHtransposase/3‑deoxy‑7‑phosphoheptulonate synthase
*NC_0029472,092,4450TG48.3% ‑3.6 / 45.7 29intergenic (+266/+652)PP_1865/aroHtransposase/3‑deoxy‑7‑phosphoheptulonate synthase
*NC_0029475,993,9100TC47.6% ‑0.5 / 26.4 21intergenic (+199/‑347)kefB‑III/PP_mr64glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
*NC_002947195,6340CT46.7% ‑3.7 / 16.3 16T380T (ACC→ACTPP_0168putative surface adhesion protein
*NC_0029472,092,4470AC46.7% ‑4.5 / 47.3 30intergenic (+268/+650)PP_1865/aroHtransposase/3‑deoxy‑7‑phosphoheptulonate synthase
*NC_0029474,438,4660GC45.8% 24.8 / 13.7 24intergenic (‑29/‑89)PP_5648/PP_3928hypothetical protein/hypothetical protein
*NC_0029473,452,2830TG45.2% 5.7 / 10.4 33A19A (GCT→GCGPP_5556bacteriophage lysis protein
*NC_0029473,796,4500TG44.1% 25.6 / 17.7 35V301G (GTA→GGA) PP_3353sulfatase‑modifying protein
*NC_002947699,7250AG41.9% 13.0 / 35.3 31intergenic (+115/‑129)PP_t06/PP_23SDtRNA‑Ala/23S ribosomal RNA
*NC_0029474,813,1530GT41.5% 45.4 / 42.4 41intergenic (‑268/‑287)PP_4234/dsbD‑IIbifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2
*NC_0029474,039,7930CA34.4% 41.6 / 12.5 32intergenic (‑97/‑150)PP_3556/PP_3557membrane protein/methyl‑accepting chemotaxis transducer
*NC_0029474,353,6980AG31.8% 26.9 / 16.4 22intergenic (‑70/+404)PP_3820/galUgroup II intron‑encoding maturase/UTP‑glucose‑1‑phosphate uridylyltransferase
*NC_0029475,993,8080GT31.6% 27.9 / 13.4 19intergenic (+97/‑449)kefB‑III/PP_mr64glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
*NC_0029473,994,3540AC28.0% 76.8 / 16.4 50T627P (ACC→CCC) oplA5‑oxoprolinase A
*NC_0029472,092,2250GT27.6% 96.8 / 31.1 59intergenic (+46/+872)PP_1865/aroHtransposase/3‑deoxy‑7‑phosphoheptulonate synthase

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_002947 16219 =10 (0.440)3 (0.140) 3/258 1.3 18.3% intergenic (+469/‑370) gyrB/PP_5421 DNA gyrase subunit B/hypothetical protein
?NC_002947 = 16362 17 (0.770)intergenic (+612/‑227) gyrB/PP_5421 DNA gyrase subunit B/hypothetical protein