Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,311,380	IS4 (+) +12 bp	noncoding (156‑167/174 nt)	psrO →	novel sRNA, function unknown

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3311380 =	0 (0.000)	27 (1.350)	17/132	0.1	100%	noncoding (156/174 nt)	psrO	novel sRNA, function unknown
	?	NC_000913	4502090 =	NA (NA)					noncoding (1426/1426 nt)	IS4	repeat region
*	?	NC_000913	= 3311391	0 (0.000)	17 (0.850)	13/132	0.3	100%	noncoding (167/174 nt)	psrO	novel sRNA, function unknown
	?	NC_000913	= 4503515	NA (NA)					noncoding (1/1426 nt)	IS4	repeat region

GCTCATCGAGCGCCTGGGTCTGCGTCGCTAATTCTTGCGAGTTTCAGAAAAGGGGGCCTGAGTGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3311445‑3311380 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTC  >  NC_000913/4502090‑4502156                                                                                                                                       GCTCATCGAGCGCCTGGGTCTGCGTCGCTAATTCTTGCGAGTTTCAGAAAAGGGGGCCTGAGTGGCTA                                                                   >  2:588155/1‑68   TCATCGAGCGCCTGGGTCTGCGTCGCTAATTCTTGCGAGTTTCAGAAAAGGGGGCCTGAGTGGCTAAT                                                                 >  2:28401/1‑68   TCATCGAGCGCCTGGGTCTGCGTCGCTAATTCTTGCGAGTTTCAGAAAAGGGGGCCTGAGTGGCTAAT                                                                 <  2:647486/68‑1        GAGCGCCTGGGTCTGCGTCGCTAATTCTTGCGAGTTTCAGAAAAGGGGGCCTGAGTGGCTAATGCCga                                                            >  2:587985/1‑66        GAGCGCCTGGGTCTGCGTCGCTAATTCTTGCGAGTTTCAGAAAAGGGGGCCTGAGTGGCTAATGCCAG                                                            >  1:416196/1‑68              CTGGGTCTGCGTCGCTAATTCTTGCGAGTTTCAGAAAAGGGGGCCTGAGTGGCTAATGCCAGTCAGTT                                                      <  1:398421/68‑1              CTGGGTCTGCGTCGCTAATTCTTGCGAGTTTCAGAAAAGGGGGCCTGAGTGGCTAATGCCAGTCAGTT                                                      <  1:517279/68‑1              CTGGGTCTGCGTCGCTAATTCTTGCGAGTTTCAGAAAAGGGGGCCTGAGTGGCTAATGCCAGTCAGTT                                                      <  1:690766/68‑1              CTGGGTCTGCGTCGCTAATTCTTGCGAGTCTCAGAAAAGGGGGCCTGAGTGGCTAATGCCAGTCAGTT                                                      <  1:458801/68‑1                        GTCGCTAATTCTTGCGAGTTTCAGAAAAGGGGGCCTGAGTGGCTAATGCCAGTCAGTT                                                      >  1:751073/1‑58                        GTCGCTAATTCTTGCGAGTTTCAGAAAAGGGGGCCTGAGTGGCTAATGCCAGTCAGTT                                                      <  2:751074/58‑1                                  CTTGCGAGTTTCAGAAAAGGGGGCCTGAGTGGCTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTT                                  >  1:465513/1‑68                                   TTGCGAGTTTCAGAAAAGGGGGCCTGAGTGGCTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTT                                 <  1:154352/68‑1                                         GTTTCAGAAAAGGGGGCCTGAGTGGCTACTGCCAGACAGATAAGCAACTGCCTGGCTCTATTTAGGGG                           >  1:169194/1‑68                                         GTTTCAGAAAAGGGGGCCTGAGTGGCTAATGCCAGTCAGTTAGGCAACTGACTGGCTCTTTTTAGGGG                           >  1:170040/1‑68                                          TTTCAGAAAAGGGGGCCTGAGTGGCTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGC                          <  2:626033/68‑1                                          TTTCAGAAAAGGGGGCCTGAGTGGCTAATGCCAGTCAGTTAAGCAACTGACTGGCGCTTTTTCGGGGC                          <  2:578468/68‑1                                           TTCAGAAAAGGGGGCCTGAGTGGCTAATGCCTGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCT                         <  2:117424/68‑1                                                 AAAGGGGGCCTGAGTGGCTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGG                   <  2:321482/68‑1                                                 AAAGGGGGCCTGAGTGGCTAATACCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGG                   <  2:623322/68‑1                                                    GGGGGCCTGAGTGGCTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTAT                >  2:272199/1‑68                                                     GGGGCCTGAGTGGCTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATT               <  2:446520/68‑1                                                            GAGTGGCTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGG        <  2:84368/68‑1                                                            GAGTGGCTAATGCCAGTCAGGTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGG        <  2:626749/68‑1                                                                 GCTTATGCCAGGCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGTGTATTTCCAGGGCCTCT   <  1:354130/68‑1                                                                 GCTAATGCCTGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGTGCTGTGGGGTATTTCCAGGGCCTCT   <  2:409762/68‑1                                                                  CTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGGGGGGTATTTCCAGGGCCTCTC  <  2:218312/68‑1                                                                                                                                       GCTCATCGAGCGCCTGGGTCTGCGTCGCTAATTCTTGCGAGTTTCAGAAAAGGGGGCCTGAGTGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3311445‑3311380 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTC  >  NC_000913/4502090‑4502156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.