Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,311,380	IS4 (+) +12 bp	noncoding (156‑167/174 nt)	psrO →	novel sRNA, function unknown

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3311380 =	0 (0.000)	27 (1.350)	17/132	0.1	100%	noncoding (156/174 nt)	psrO	novel sRNA, function unknown
	?	NC_000913	4502090 =	NA (NA)					noncoding (1426/1426 nt)	IS4	repeat region
*	?	NC_000913	= 3311391	0 (0.000)	17 (0.850)	13/132	0.3	100%	noncoding (167/174 nt)	psrO	novel sRNA, function unknown
	?	NC_000913	= 4503515	NA (NA)					noncoding (1/1426 nt)	IS4	repeat region

TAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAGGGGCCACTCAGGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3311326‑3311391 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGC  <  NC_000913/4503515‑4503454                                                                                                                                  TAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTAGAAAAAAGGGGCCACTCAGGCCTA                                                              >  1:117862/1‑68           ACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAGGGGCCACTCAGGCCTAATGCCGATCA                                                    >  1:263414/1‑68            CATAAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAGGGGCCACTCAGGCCTAATGCCGATCAG                                                   <  1:715657/68‑1              TTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAGGGGCCACTCAGGCCTAATGCCGATCAGTT                                                 <  1:243302/68‑1                AGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAGGGGCCGCTCAGGCCTAATGCCGATCAGTTAA                                               >  1:463870/1‑68                AGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAGGGGCCACTCAGGCCTAATGCCGATCAGTT                                                 <  1:291535/66‑1                AGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAGGGGCCACTCAGGCCTAATGCCGATCAGTT                                                 >  2:291535/1‑66                 GTTTCCAGTGAATTGCTGCCGGCAGCTTGAAAAAAGGGGCCACTCAGGCCTAATGCCGATCAGTTAAG                                              <  1:525119/68‑1                               GCTGCCGTCAGCTTGAAAAAAGGGGCCACTCAGGCCTAATGCCGATCAGTTAAGGATCAGTTGACCGA                                <  2:512791/68‑1                                CTGCCGTCAGCTTGAAAAAAGGGGCCACTCAGGCCTAATGCCGATCAGTTAAGGATCAGTTGACCGAT                               <  1:201984/68‑1                                         GCTTGAAAAAAGGGGCCACTCAGGCCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCT                      <  2:263414/68‑1                                           TTGAAAAAAGGGGCCACTCAGGCCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGT                    <  1:541395/68‑1                                                            TCAGGCCTAATGCCGATCAGTTAAGGATCAGTTGACCGCTCCAGTGGCTGTGTAAGAATCCGGAAACG   >  2:1690/1‑68                                                             CAGGCCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAAAATCCGGAAACaa  <  1:149784/68‑3                                                             CAGGCCTAATGCCGATCAGTTAAGGATCAGTTGACCGAGCCAGTGGCTGTGTAAGAATCCGGAAACaa  <  1:268001/68‑3                                                                 CCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAAT            <  1:52920/54‑1                                                                 CCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAAT            >  2:52920/1‑54                                                                                                                                  TAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAGGGGCCACTCAGGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3311326‑3311391 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGC  <  NC_000913/4503515‑4503454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.