breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 547,694 A→G intergenic (+123/‑1156) fdrA → / → ylbF acyl‑CoA synthetase with NAD(P)‑bindingRossmann‑fold domain/hypothetical protein
RA 547,835 +G intergenic (+264/‑1015) fdrA → / → ylbF acyl‑CoA synthetase with NAD(P)‑bindingRossmann‑fold domain/hypothetical protein
RA 556,858 A→T L36Q (CTG→CAG)  folD ← bifunctional 5,10‑methylene‑tetrahydrofolatedehydrogenase/5,10‑methylene ‑tetrahydrofolatecyclohydrolase
MC JC 654,214 Δ1,199 bp insH_12 insH_12
RA 987,574 G→T intergenic (‑170/+433) ompF ← / ← asnS outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase
RA 1,019,399 C→A intergenic (+40/+36) matP → / ← ompA hypothetical protein/outer membrane protein A (3a;II*;G;d)
RA 1,093,686 T→C V130A (GTA→GCA)  ycdT → diguanylate cyclase
RA 1,197,797 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase,‑specific for NADP+
RA 1,197,809 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase,‑specific for NADP+
RA 1,197,822 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase,‑specific for NADP+
RA 1,197,824 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase,‑specific for NADP+
RA 1,197,854 C→T N385N (AAC→AAT icd → isocitrate dehydrogenase,‑specific for NADP+
RA 1,197,857 G→C A386A (GCG→GCC icd → isocitrate dehydrogenase,‑specific for NADP+
RA 1,197,860 A→G K387K (AAA→AAG icd → isocitrate dehydrogenase,‑specific for NADP+
MC JC 1,301,041 Δ1,336 bp insC_8insD_10 insC_8, insD_10
RA 1,519,222 G→T V40V (GTG→GTT ydcX → inner membrane protein
RA 1,669,601 Δ1 bp coding (678/1221 nt) dgsA ← DNA‑binding transcriptional repressor
RA 1,979,957 G→T intergenic (‑37/+398) flhD ← / ← insH_10 DNA‑binding transcriptional dual regulator/IS5 element protein
RA 2,005,401 C→T E115K (GAA→AAA)  fliC ← flagellar filament structural protein
RA 2,074,160 C→A A162E (GCG→GAG)  flu → antigen 43 (Ag43) phase‑variable biofilmformation autotransporter
MC JC 2,176,983 Δ1,199 bp insH_11 insH_11
RA 2,245,455 A→G S283P (TCG→CCG)  yeiB ← inner membrane protein
JC 2,288,259 (TTATCGCCT)2→3 coding (550/1761 nt) yejM → hydrolase, inner membrane
MC JC 2,320,795 Δ1,336 bp insD_9insC_5 insD_9, insC_5
RA 2,866,111 A→G intergenic (‑21/+159) rpoS ← / ← nlpD RNA polymerase, sigma S (sigma 38) factor/outer membrane lipoprotein
RA 2,935,326 C→A R363S (CGT→AGT)  fucI → L‑fucose isomerase
MC JC 3,261,134 Δ1,199 bp insH_16[tdcD] insH_16, [tdcD]
RA 3,268,825 A→G T186A (ACA→GCA)  yhaC → hypothetical protein
RA 3,423,631 G→T noncoding (12/120 nt) rrfD ← 5S ribosomal RNA
RA 3,453,401 G→A A679V (GCA→GTA)  rpoB ← RNA polymerase subunit beta
RA 3,469,815 A→G noncoding (208/1542 nt) rrsB ← 16S ribosomal RNA
RA 3,542,160 A→G intergenic (+202/‑249) rhaA → / → rhaD L‑rhamnose isomerase/rhamnulose‑1‑phosphate aldolase
RA 3,542,162 A→G intergenic (+204/‑247) rhaA → / → rhaD L‑rhamnose isomerase/rhamnulose‑1‑phosphate aldolase
RA 3,542,165 T→C intergenic (+207/‑244) rhaA → / → rhaD L‑rhamnose isomerase/rhamnulose‑1‑phosphate aldolase
RA 3,542,182 A→G intergenic (+224/‑227) rhaA → / → rhaD L‑rhamnose isomerase/rhamnulose‑1‑phosphate aldolase
RA 3,542,266 A→C intergenic (+308/‑143) rhaA → / → rhaD L‑rhamnose isomerase/rhamnulose‑1‑phosphate aldolase
MC JC 3,742,936 Δ1,336 bp insD_8insC insD_8, insC
MC JC 3,746,911 Δ1,199 bp insH_13 insH_13
MC JC 3,750,591 Δ1,199 bp insH_14 insH_14
MC JC 3,958,123 Δ34 bp coding (1156‑1189/1287 nt) dctA → C4‑dicarboxylic acid, orotate and citratetransporter
RA 4,255,957 G→T intergenic (+84/‑139) yjbI → / → ubiC hypothetical protein/chorismate pyruvate lyase
MC JC 4,310,363 Δ1,199 bp insH_15[alsK] insH_15, [alsK]
RA 4,371,274 (A)5→3 intergenic (‑9/+296) dcuA ← / ← aspA C4‑dicarboxylate antiporter/aspartate ammonia‑lyase
RA 4,567,429 C→T S321S (TCG→TCA yjiK ← hypothetical protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_007779_cfb 1197863 1213089 15227 67 [65] [66] 68 [icd]–mcrA [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,cohE,croE,ymfL,ymfM,ymfN,ymfR,ymfO,ymfP,ymfQ,stfP,tfaP,tfaE,pinE,mcrA
* * ÷ NC_007779_cfb 1395926 1398871–1397761 1836–2946 172 [2] [66] 67 [mppA]–insH_8 [mppA],ynaI,insH_8
* * ÷ NC_007779_cfb 3597427–3597969 3601408–3600948 2980–3982 69 [66] [66] 67 [rrlA]–rrsA [rrlA],alaT,ileT,rrsA

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_007779_cfb 380484 =NA (NA)138 (0.990) 109/438 0.4 NA intergenic (‑418/‑46) yaiO/insC_2 hypothetical protein/IS2 insertion element repressor InsA
?NC_007779_cfb = 381407 NA (NA)coding (510/906 nt) insD IS2 insertion element transposase InsAB'
* ? NC_007779_cfb 381401 =NA (NA)144 (1.040) 118/434 0.2 NA coding (504/906 nt) insD IS2 insertion element transposase InsAB'
?NC_007779_cfb = 381812 NA (NA)coding (303/387 nt) yaiF hypothetical protein
* ? NC_007779_cfb = 13959252 (0.010)134 (0.970) 114/432 0.2 98.6% coding (985/1614 nt) mppA murein tripeptide(L‑ala‑gamma‑D‑glutamyl‑meso‑DAP) transporter subunit
?NC_007779_cfb 1397761 = NA (NA)intergenic (‑125/‑29) ynaI/insH_8 inner membrane protein/IS5 transposase and trans‑activator
* ? NC_007779_cfb = 3186081NA (NA)158 (1.140) 128/436 0.1 98.8% coding (26/2466 nt) yqiG outer membrane usher protein
?NC_007779_cfb 3512753 = 2 (0.010)coding (529/1026 nt) cytR DNA‑binding transcriptional dual regulator
* ? NC_007779_cfb = 35127596 (0.040)113 (0.810) 101/436 0.6 95.0% coding (535/1026 nt) cytR DNA‑binding transcriptional dual regulator
?NC_007779_cfb = 3744262 NA (NA)intergenic (‑90/+31) insC/yidZ IS2 element protein/DNA‑binding transcriptional regulator
* ? NC_007779_cfb = 3744263NA (NA)155 (1.110) 119/438 0.2 95.7% intergenic (‑91/+30) insC/yidZ IS2 element protein/DNA‑binding transcriptional regulator
?NC_007779_cfb 3903274 = 7 (0.050)coding (37/825 nt) bax hypothetical protein
* ? NC_007779_cfb = 39032790 (0.000)142 (1.020) 115/436 0.2 100% coding (42/825 nt) bax hypothetical protein
?NC_007779_cfb = 4504190 NA (NA)intergenic (+10/‑89) insD_3/yjgW IS2 insertion element transposase InsAB'/hypothetical protein
* ? NC_007779_cfb = 3988378NA (NA)133 (0.960) 112/436 0.3 99.3% coding (747/783 nt) yhiS hypothetical protein
?NC_007779_cfb = 4365922 1 (0.010)coding (691/1539 nt) cadC DNA‑binding transcriptional activator
* ? NC_007779_cfb 4310363 =NA (NA)185 (1.330) 134/438 0.1 97.4% intergenic (‑287/‑68) yjcS/insH_15 alkyl sulfatase/IS5 element protein
?NC_007779_cfb = 4342649 5 (0.040)intergenic (‑42/+283) adiY/adiA DNA‑binding transcriptional activator/biodegradative arginine decarboxylase
* ? NC_007779_cfb 4310363 =NA (NA)140 (1.000) 110/438 0.4 97.2% intergenic (‑287/‑68) yjcS/insH_15 alkyl sulfatase/IS5 element protein
?NC_007779_cfb 4365918 = 4 (0.030)coding (695/1539 nt) cadC DNA‑binding transcriptional activator
* ? NC_007779_cfb = 4311556NA (NA)146 (1.050) 98/436 0.7 98.0% coding (922/1029 nt) alsK D‑allose kinase
?NC_007779_cfb 4540915 = 3 (0.020)intergenic (+204/+379) yjhR/nanS frameshift suppressor/hypothetical protein
* ? NC_007779_cfb = 4311557NA (NA)81 (0.580) 65/438 2.6 97.6% coding (921/1029 nt) alsK D‑allose kinase
?NC_007779_cfb 4342646 = 2 (0.010)intergenic (‑39/+286) adiY/adiA DNA‑binding transcriptional activator/biodegradative arginine decarboxylase