breseq  version 0.32.0b  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 468,093 Δ1 bp coding (40/339 nt) glnK → nitrogen assimilation regulatory protein for GlnL, GlnE, and AmtB
RA 1,075,312 C→T S184S (AGC→AGT putP → proline:sodium symporter
RA 1,738,537 A→T Q275L (CAG→CTG)  mdtK → multidrug efflux system transporter
RA 2,054,766 C→A G217C (GGT→TGT)  nac ← nitrogen assimilation regulon transcriptional regulator; autorepressor
RA 2,440,860 C→A P31P (CCG→CCT aroC ← chorismate synthase
RA 2,667,677 G→A A124V (GCG→GTG)  yphB ← mutarotase superfamily protein, YphB family
RA 2,718,735 G→T P124Q (CCG→CAG)  kgtP ← alpha‑ketoglutarate transporter
RA 2,719,426 T→C intergenic (‑321/+2) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
RA 3,960,035 C→G R87G (CGC→GGC)  rho → transcription termination factor
RA 4,142,582 G→A H149Y (CAC→TAC)  ppc ← phosphoenolpyruvate carboxylase
RA 4,390,235 T→C I44T (ATC→ACC)  hfq → global sRNA chaperone; HF‑I, host factor for RNA phage Q beta replication

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP009273 345710 346909 1200 21 [19] [19] 20 prpC prpC
* * ÷ CP009273 748599 749949 1351 20 [19] [19] 20 gltA gltA

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 360104 =NA (NA)113 (2.070) 72/296 0.0 NA intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 NA (NA)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 = 104600149 (0.900)50 (0.920) 40/296 0.4 64.1% intergenic (+15/+418) insB1/cspH IS1 transposase B/stress protein, member of the CspA‑family
?CP009273 2269338 = 7 (0.130)coding (587/1026 nt) yejE microcin C transporter YejABEF, permease subunit; ABC family
* ? CP009273 1203246 =8 (0.150)24 (0.490) 23/264 1.4 78.5% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 1205075 = 6 (0.120)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 12032618 (0.150)44 (0.910) 36/264 0.4 87.0% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 = 1205058 6 (0.120)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 1391495NA (NA)50 (0.920) 41/296 0.3 100% intergenic (+146/‑127) insH1/ynaJ IS5 transposase and trans‑activator/DUF2534 family putative inner membrane protein
?CP009273 = 1972751 0 (0.000)intergenic (‑297/‑483) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
* ? CP009273 1972748 =0 (0.000)51 (0.940) 35/294 0.6 100% intergenic (‑294/‑486) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
?CP009273 = 2283592 NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? CP009273 = 22693463 (0.060)45 (0.830) 36/296 0.6 93.8% coding (595/1026 nt) yejE microcin C transporter YejABEF, permease subunit; ABC family
?CP009273 3576788 = NA (NA)intergenic (+10/‑55) yrhA/insA pseudogene, interrupted by IS1E/IS1 repressor TnpA