Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	CP009273	299,113	IS5 (–) +4 bp	coding (412‑415/615 nt)	yagU →	DUF1440 family inner membrane protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP009273	269666 =	NA (NA)	49 (1.090)	30/296	0.6	100%	noncoding (1195/1195 nt)	IS5	repeat region
	?	CP009273	= 299116	0 (0.000)					coding (415/615 nt)	yagU	DUF1440 family inner membrane protein
*	?	CP009273	= 270860	NA (NA)	40 (0.890)	32/296	0.5	100%	noncoding (320/1221 nt)	IS30	repeat region
	?	CP009273	299113 =	0 (0.000)					coding (412/615 nt)	yagU	DUF1440 family inner membrane protein

ACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP009273/270717‑270860 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTAGCCCAACTTTTTGTTCATATGATTTCATTCCCTCTCATGGGACTGACGCCACCTCTGTTTGATCTCCCGTGGTATGAGAATGTTTCTGAAATTTTTGGACATTTAGTCTGGTTCTGGTCTATTGAAATTATTCGCAGAGATT  >  CP009273/299113‑299257                                                                                                                                                                                                                                                                                                   ACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGC                                                                                                                                              >  2:280591/1‑149  CAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCC                                                                                                                                             <  1:196131/149‑1               TTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCCCAACTTTTTGTTC                                                                                                                                <  2:460861/149‑1                      gCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCCCAACTTTTTGTTCATATGAT                                                                                                                         <  2:63523/148‑1                                 TTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCCCAACTTTTTGTTCATATGATTTCATTCCCTC                                                                                                              >  2:92776/1‑149                                 TTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTAATGATGAACCCTGTTCTATGGCTGCAGATGACAAACATGACCTCATAACAGGGACATGTTCGCACCTTCCTTAGCCCAACTTTTTGTTCATATGATTTCATCCCCTC                                                                                                              >  2:86478/1‑149                                     TGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCCCAACTTTTTGTTCATATGATTTCATTCCCTCTCAT                                                                                                          >  1:672870/1‑149                                     TGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCCCAACTTTTTGTTCATATGATTTAATTCCCTCTCAT                                                                                                          >  2:591761/1‑149                                     TGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGCCCAACTTTTTGTTCATATGATTTCATTCCCTCTCAT                                                                                                          >  1:735500/1‑149                                     TGCTGAATTCACTGTCGGAGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGTCTTGTTCGCACCTTCCTTAGCCCAACTTTTTGTTCATATGATTTCATTCCCTGTCAT                                                                                                          >  2:194008/1‑149                                      GCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCCCAACTTTTTGTTCATATGATTTCATTCCCTCTCATG                                                                                                         >  1:510570/1‑149                                         GAACTCGCTGTCGTCGAAGTCAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCCCCTTCCTTAGCCCAACTTTTTGTTCATATGATTTCATTCCCTCTCATGGGA                                                                                                      <  2:3406/149‑1                                            TTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCCCAACTTTTTGTTCATATGATTTCATTCCCTCTCATGGGACTG                                                                                                   >  2:421130/1‑149                                            TTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCCCAACTTTTTGTTCATATGATTTCATTCCCTCTCATGGGACTG                                                                                                   >  2:186205/1‑149                                               ACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCCCAACTTTTTGTTCATATGATTTCATTCCCTCGCATGGGACTGACG                                                                                                >  1:576275/1‑149                                                CTGCCGGCGAAGGTAAGTTGAGGACTCAGGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCCCAACTTTTCGTTCATATGATTTCATTCCCTCTCATGGGACTGACGC                                                                                               <  2:574466/149‑1                                                 TGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCCCAACTTTTTGTTCATATGATTTCATTCCCTCTCATGGGACTGCCGCC                                                                                              >  2:631582/1‑149                                                   TCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCCCAACTTTTTGTTCATATGATTTCATTCCCTCTCATGGGACTGACGCCAC                                                                                            >  2:498852/1‑149                                                     GGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCCCAACTTTTTGTTCATATGATTTCGTTCCCTCTCATGGGACTGACGCCGCCT                                                                                          >  2:275772/1‑149                                                        GAAGGTAAGTTGATGACTCATGATGAACCCGGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTGCCTTAGCCCCACTTTTTGTTCATATGATTTCATTCCCTCTCATGTGACTGACGCCACCTCTG                                                                                       >  2:507230/1‑149                                                              AAGTTGACCACCCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCCCAACTTTTTGTTCATATGATTTCATTCCCTCTCATGGGACTGACGCCACCTCTGTTTGAT                                                                                 <  2:382037/149‑1                                                                GTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCCCAACTTTTTGTTCATATGATTTCATTCCCTCTCATGGGACTGACGCCACCTCTGTTTGATCT                                                                               >  2:331689/1‑149                                                                        CTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCCCAACTTTTTGTTCATATGATTTCATTCCCTCTCATGGGACTGACGCCACCTCTGTTTGATCTCCCGTGGT                                                                       <  1:361529/149‑1                                                                         TCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCCCAACTTTTTGTTCATATGATTTCATTCCCTCTCATGGGACTGACGCCACCTCTGTTTGATCTCCCGTGGTA                                                                      >  1:569351/1‑149                                                                         TCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCCCAACTTTTTGTTCATATGATTTCATTCCCTCTCATGGGACTGACGCCACCTCTGTTTGATCTCCCGTGGTA                                                                      >  1:31447/1‑149                                                                                  ACCCTGTTCTATGGCTCCAGATGACAAACATGATCTAATATCAGGGACTTGTTCGCACCTTCCTTAGCCCAACTTTTTGTTCATATGATTTCATTCCCTCTCATGGGACTGACGCCACCTCTGTTTGATCTCCCGTGGTATGAGAATGT                                                             <  2:308481/149‑1                                                                                   CCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTATTCGCATCTTCCTTAGCCCAACTTTTTGTTCATATGATTTCATTCCCTCTCATGGGACTGACGCCACCTCTGTTTGATCTCCCGTGGTATGAGAATGTT                                                            >  2:480111/1‑149                                                                                    CCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCCCAACTTTTTGTTCATATGATTTCATTCCCTCTCATGGGACTGACGCCACCTCTGTTTGATCTCCCGTGGTATGAGAATGTTT                                                           <  1:326881/149‑1                                                                                       GTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCCCAACTTTTTGTTCATATGATTTCATTCCCTGTCATGGGACGGACGCCACCTCTGCTTGCGCGCCCGTGGTATGAGAATGTTTCTG                                                        >  2:481583/1‑149                                                                                                CTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCCCAACTTTTTGTTCATATGATTTCATTCCCTCTCATGGGACTGACGCCACCTCTGTTTGATCTCCCGTGGTATGAGAATGTTTCTGAAATTTTTG                                               >  1:658285/1‑149                                                                                                    AGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCCCAACTTTTTGTTCATATGATTTCATTCCCTCTCATGGGCCTGTCTCTTTCGCTTTTTGATCTCCCGTGGTATGAGAATGTTTCTGAAATTTTTGGACA                                           >  1:571564/1‑149                                                                                                               ATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCCCAACTTTTTGTTCATATGATTTCATTCCCTCTCATGGGACTGACGCCACCTCTGTTTGATCTCCCGTGGTATGAGAATGTTTCTGAAATTTTTGGACATTTAGTCTGGT                                >  2:483044/1‑149                                                                                                                     TCATATCAGGGACTTGTTCGCACCTTCCTTAGCCCAACTTTTTGTTCATATGATTTCATTCCCTCTCATGGGACTGACGCCCCCTCTGTTTGCTCTCCCGTGGTATGAGAATGTTTCTGAAATTTTTGGACATTTCGTCTGGTTCTGGg                          >  2:119778/1‑148                                                                                                                        TATCAGGGACTTGTTCGCACCTTCCTTAGCCCAACTTTTTGTTCATATGATTTCATTCCCTCTCATGGGACTGACGCCACCTCTGTTTGATCTCCCGTGGTATGAGAATGTTTCTGAAATTTTTGGACATTTAGTCTGGTTCTGGTCTA                       >  1:393753/1‑149                                                                                                                            AGGGACTTGTTCGCACCTTCCTTAGCCCAACTTTTTGTTCATATGATTTCATTCCCTCTCATGGGACTGACGCCACCTCTGTTTGATCTCCCGTGGTATGAGAATGTTTCTGAAATTTTTGGACATTTAGTCTGGTTCTGGTCTATTGA                   >  1:185785/1‑149                                                                                                                                       CGCACCTTCCTTAGCCCAACTTTTTGTTCATATGATTTCATTCCCTCTCATGGGACTGACGCCACCTCTGTTTGATCTCCCGTGGTATGAGAATGTTTCTGAAATTTTTGGACATTTAGTCTGGTTCTGGTCTATTGAAATTATTCGCA        >  1:374828/1‑149                                                                                                                                        gaACCTTCCTTAGCCCAACTTTTTGTTCATATGATTTCATTCCCTCTCATGGGACTGAAGCCACCTCTGTTTGATCTCCCGTGGTATGAGAATGTTTCTGAAATTTTTGGCCATTTAGTCTGGATCTGGTCTATTGAAATTActggcgg       >  2:193767/3‑142                                                                                                                                          ACCTTCCGTAGCCCAACTTTTTGTTCATATTATTCCCTTCCCGCTCATGGGGCTTACGCCACCTCTGTTTGATCTCCCGTGGTATGAGAATGTTTCTGAAATTTTTGGACATTTATTCTGGTTCTGGTCTATTGAAATTATTCGCAGAG     <  2:120504/149‑1                                                                                                                                           CCTTCCTTAGCCCAACTTTTTGTTCATATGATTTCATTCCCTCTCATGGGACTGACGCCCCCTCTGTTTGATCTCCCGTGGTATGCCAATGTTTCAGAAATTTTTTGACATTTAGTCTTTTTCTGTTCTATTGAAATTATTCGCAGAGA    <  2:510570/149‑1                                                                                                                                             TTCCTTAGCCCAACTTTTTGTTCATATGATTTCATTCCCTCTCATGGGACTGACGCCACCTCTGTTTGATCTCCCGTGGTATGAGAATGTTTCTGAAATTTTTGGACATTTAGTCTGGTTCTGGTCTATTGAAATTATTCGCAGAGATT  >  2:695143/1‑149                                                                                                                                                                                                                                                                                                   ACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP009273/270717‑270860 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTAGCCCAACTTTTTGTTCATATGATTTCATTCCCTCTCATGGGACTGACGCCACCTCTGTTTGATCTCCCGTGGTATGAGAATGTTTCTGAAATTTTTGGACATTTAGTCTGGTTCTGGTCTATTGAAATTATTCGCAGAGATT  >  CP009273/299113‑299257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.