Predicted mutation
evidence seq id position mutation annotation gene description
JC JC CP009273 299,113 IS5 (–) +4 bp coding (412‑415/615 nt) yagU → DUF1440 family inner membrane protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 269666 =NA (NA)49 (1.090) 30/296 0.6 100% noncoding (1195/1195 nt) IS5 repeat region
?CP009273 = 299116 0 (0.000)coding (415/615 nt) yagU DUF1440 family inner membrane protein
* ? CP009273 = 270860NA (NA)40 (0.890) 32/296 0.5 100% noncoding (320/1221 nt) IS30 repeat region
?CP009273 299113 = 0 (0.000)coding (412/615 nt) yagU DUF1440 family inner membrane protein

GACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCATTCTGTTAACAAACGCGGTATAACAAACCTTCTTTGGAT  >  CP009273/270716‑271005
                                                                                                                                                |                                                                                                                                                 
gACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACCTGATCTCATATCAGGGACTTGTTCGAACCTTGCGGAg                                                                                                                                               >  1:122834/1‑149 (MQ=12)
  cAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGc                                                                                                                                             <  2:369565/149‑1 (MQ=34)
       aaaTCGCTTTTCTGTTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTAt                                                                                                                                        <  2:536415/149‑1 (MQ=255)
         aTCTCTTTTCGGGTCTGACGGCGCGTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGa                                                                                                                                      <  2:365857/149‑1 (MQ=255)
         aTCTCTTCTCTGGTCTGACGGCGATTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCACATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGa                                                                                                                                      >  2:501476/1‑149 (MQ=255)
          tctcTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAg                                                                                                                                     <  1:116767/149‑1 (MQ=40)
                tccgggTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTggcg                                                                                                                               <  2:698237/145‑1 (MQ=255)
                            ggCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTa                                                                                                                   <  1:169266/149‑1 (MQ=255)
                                cTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAGCCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTg                                                                                                               <  1:52349/149‑1 (MQ=255)
                                     tgctgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGattat                                                                                                          >  1:78790/1‑149 (MQ=255)
                                       ctgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGAttttta                                                                                                        >  1:308550/1‑149 (MQ=255)
                                         gAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGCGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGGTTATTAAt                                                                                                      >  1:162327/1‑149 (MQ=255)
                                            ttCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATACCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATccc                                                                                                   >  1:210820/1‑149 (MQ=255)
                                                 tGTCGGCGAAGGTAAGTTGATGATTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCAccc                                                                                              <  1:200488/149‑1 (MQ=255)
                                                 tGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTCTGGCGCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCGTTTTGGTTCTGATCATTAATTCCCAccc                                                                                              >  2:305978/1‑149 (MQ=18)
                                                   tcgcctaaGGTAAGCTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGc                                                                                            <  1:198693/143‑1 (MQ=255)
                                                              aaGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGcc                                                                                 >  2:2841/1‑149 (MQ=255)
                                                              aaGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGACCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGcc                                                                                 >  2:611812/1‑149 (MQ=255)
                                                                gTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCg                                                                               <  2:582824/149‑1 (MQ=255)
                                                                 ttGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTAGCTGATTATAAATCCCCACCCGCTATTAAGCGCCCgg                                                                              >  1:433650/1‑149 (MQ=255)
                                                                      gaCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCgg                                                                         <  1:116376/149‑1 (MQ=255)
                                                                       aCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCggg                                                                        >  2:723245/1‑149 (MQ=255)
                                                                               tgaACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTTGCGGCCCTTTTTGTATCTGATTATTAATCCCCCCCCGCTATTAAGCGCCCGGCGCGGGCATCTGCg                                                                >  2:169112/1‑149 (MQ=255)
                                                                                   cccTGTGCTATGGCCCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTCTGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCCCCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTg                                                            <  1:193987/149‑1 (MQ=255)
                                                                                        ttttATGGCTCCAGATGACAAACATGTTCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCa                                                       <  2:471078/146‑1 (MQ=255)
                                                                                        ttCTATGTCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCa                                                       <  1:376251/149‑1 (MQ=255)
                                                                                        ttCCATGCCTCCAGCTGACAAACATGATCTCCTATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCa                                                       <  1:376207/149‑1 (MQ=255)
                                                                                            aTGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCCggtt                                                   >  2:392525/1‑147 (MQ=255)
                                                                                                   cAGATGACAAACGTGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACttt                                            <  2:88631/149‑1 (MQ=255)
                                                                                                     gacgACAAATCTGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCCGCGTCTGGTGCAGGGTTGACTTTGc                                          <  1:380865/146‑1 (MQ=255)
                                                                                                         aCAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGACCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCATTc                                      <  1:611812/149‑1 (MQ=255)
                                                                                                           aaaCATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCATTCTg                                    <  1:200592/149‑1 (MQ=255)
                                                                                                            aaCATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCATTCTGt                                   <  1:714287/149‑1 (MQ=255)
                                                                                                               aTGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCATTCTGTTaa                                <  1:713795/149‑1 (MQ=255)
                                                                                                                   tctcATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCATTCTGTTAACaaa                            >  2:651044/1‑149 (MQ=255)
                                                                                                                    ctcATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCATTCTGTTAACAAAc                           >  2:8432/1‑149 (MQ=255)
                                                                                                                             gggACTTGTTCGCACCTTCCGGAGGCGTTATGTGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCATTCTGTTAATAAACGCGGTATaa                  <  1:501476/149‑1 (MQ=255)
                                                                                                                                 cTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTATGCATTCTGTTAACAAACGCGGTATAACaaa              <  1:360577/149‑1 (MQ=255)
                                                                                                                                 cTTGCTCCCACCTTCGGGAGGCGTTCTGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCTGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCATTCTGTTAACAAACGCGGTATAACaaa              <  1:345290/149‑1 (MQ=255)
                                                                                                                                  tttcccGCTCCTCCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAACCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGTTTGACTTTGCATTCTGTTAACAAACGCGGTATAACAAAc             <  2:708383/144‑1 (MQ=255)
                                                                                                                                    gTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCATTCTGTTAACAAACGCGGTATAACAAACct           <  1:2841/149‑1 (MQ=255)
                                                                                                                                            cTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCATTCTGTTAACAAACGCGGTATAACAAACCTTCTTTGGa   >  1:676754/1‑149 (MQ=255)
                                                                                                                                             ttCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCATTCTGTTAACAAACGCGGTATAACAAACCTTCTTTGGAt  <  2:613105/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
GACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCATTCTGTTAACAAACGCGGTATAACAAACCTTCTTTGGAT  >  CP009273/270716‑271005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.