Predicted mutation
evidence seq id position mutation annotation gene description
JC JC CP009273 299,113 IS5 (–) +4 bp coding (412‑415/615 nt) yagU → DUF1440 family inner membrane protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 269666 =NA (NA)49 (1.090) 30/296 0.6 100% noncoding (1195/1195 nt) IS5 repeat region
?CP009273 = 299116 0 (0.000)coding (415/615 nt) yagU DUF1440 family inner membrane protein
* ? CP009273 = 270860NA (NA)40 (0.890) 32/296 0.5 100% noncoding (320/1221 nt) IS30 repeat region
?CP009273 299113 = 0 (0.000)coding (412/615 nt) yagU DUF1440 family inner membrane protein

CCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTG‑CGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTT  >  CP009273/269527‑269811
                                                                                                                                           |                                                                                                                                                  
ccccATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTG‑Cg                                                                                                                                          >  2:347450/1‑149 (MQ=255)
 cccATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAACCTTCGCCGCATCGCTGCCAGCACTGTTATGGACGGGG‑CGa                                                                                                                                         >  2:576593/1‑149 (MQ=255)
         aaTGAGGGCTTTAGCTGTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTG‑CGAATAAGCgg                                                                                                                                 <  2:87181/149‑1 (MQ=255)
            gccGGCTTTATCTTTCTGGGGCACGGGCTCGTTCGCAAACGCAGTCGTTAGGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTGCGCCGCAGCGCTGACAGCACTGTGATGGAAGGTG‑CGAATAAGCGGGGa                                                                                                                              <  2:463573/147‑1 (MQ=255)
            gACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTCATGGCGTGATGCGAGGGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTCTGGAAGGTG‑CGAATAAGCGGGGa                                                                                                                              >  2:485072/1‑149 (MQ=255)
                     aTCTTTCGGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTG‑CGAATAAGCCGGGAAATtcttct                                                                                                                     <  1:501630/149‑1 (MQ=255)
                        tttCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGACGGTG‑CGAATAAGCGGGGAAATCCTTCTCgg                                                                                                                  >  2:427068/1‑149 (MQ=255)
                            tGGGGCCCAGGATCATTCGGAGACGCAGTCGTCTTGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTG‑CGAATAAGCGGGGAAATTCTTCTCGGCTGa                                                                                                              <  1:14995/149‑1 (MQ=38)
                              gggCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTG‑CGAATAAGCGGGGAAATTCTTCTCGGCTGACt                                                                                                            >  1:267291/1‑149 (MQ=255)
                                  acaGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTG‑CGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGt                                                                                                        <  1:558092/149‑1 (MQ=255)
                                  acaGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTG‑CGAATAAGCGGGGAAATTCTTCGCGGCTGCCTCAGt                                                                                                        >  1:388723/1‑149 (MQ=255)
                                          aTTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTG‑CGAATAAGCGGGGAAATTCTTCTCGGCTGCCTCAGtcatttcat                                                                                                >  2:213755/1‑149 (MQ=255)
                                           ttCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCCTCGCTGACAGCACTGTGATGGAAGGTG‑CGAATAAGCGGGGGAATTCTTCTCGGCTGACTCAGtcatttcatt                                                                                               >  2:719133/1‑149 (MQ=255)
                                                aaaCGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTGGCCGCATCGCTGACAGCACTGTTATGGAAGGTG‑CGAATAAGCGGGGAAATTCTTCTCGGCTGGCTCCGTCATCTCATtgcttc                                                                                          >  2:42631/1‑149 (MQ=38)
                                                 aaCGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCCGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTG‑CGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCa                                                                                         >  2:473137/1‑149 (MQ=255)
                                                  aCGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTG‑CGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCAt                                                                                        >  1:625390/1‑149 (MQ=255)
                                                                gCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTG‑CGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAttt                                                                          <  1:392487/149‑1 (MQ=255)
                                                                            tGGTCTCAACGAACCCCCAGGAAAAACCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTG‑CGAATCAGCGGGGAAAATCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTCTTTCTCCCGTaa                                                              <  2:454300/149‑1 (MQ=21)
                                                                                 tcAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTG‑CGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGcc                                                         >  1:642936/1‑149 (MQ=255)
                                                                                   aaCGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTG‑CGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTACATGCCtt                                                       >  2:315490/1‑149 (MQ=255)
                                                                                     cGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGGCGCGATGTGGAAGGTG‑CGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGa                                                     <  1:606063/149‑1 (MQ=18)
                                                                                      gccgccGCGGGATAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTCATGGAAGGTG‑CGAATAAGCGGGGACATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGaa                                                    <  2:619972/145‑1 (MQ=18)
                                                                                          ccGCAGGAGCAAGCCAGCCACTCCGCCGCCCCACCGCAACCACT‑TCATGGCAGGCGCCTAATGAGCGGGGGAACTCGTCCCGGCGGGCCCAGGCATTTCGTTTCTGCAGGTTTGAGCCGCTTTTTTCGCCCGTAAATGCCTTGAATCAg                                                >  2:329795/1‑149 (MQ=2)
                                                                                          ccGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTG‑CGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAg                                                <  1:187389/149‑1 (MQ=255)
                                                                                           cGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTG‑CGAATAAGCGGGGAAATTCTTCTCGGCTGACTAAGTCATTTCATTTCTTCATGGTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGGATCatc                                               >  2:508058/1‑147 (MQ=255)
                                                                                                   aaaGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTG‑CGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGCTTGAGCCGGTTTTTTCTCCCGTAAATTCCTTGAATCAGCCGATTTAg                                       >  1:686866/1‑149 (MQ=255)
                                                                                                        cAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTG‑CGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTACTTAGACCGt                                  >  1:39750/1‑149 (MQ=255)
                                                                                                          gAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTG‑CGAATAAGCGGGGAAATTCTTCTCGGCTGACTCCGTCATATCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTCTTTAGACCGatt                                >  2:469419/1‑149 (MQ=255)
                                                                                                                 cgccgcATCGCTGACAGCACTGTTATGGAAGGTG‑CGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCGTATTTAGACCGTTTCTTCGcc                         >  2:71454/1‑149 (MQ=255)
                                                                                                                     gcATCGCTGACAGCACTGTTATGGAAGGTG‑CGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCGTTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCACCCTATTTAGACCGTTTCTTCGCCtttt                     >  2:172734/1‑149 (MQ=255)
                                                                                                                     gcATCGCTGACAGCACTGTTATGGAAGGTG‑CGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTCTCTTCGCCAttt                     >  2:538409/1‑149 (MQ=255)
                                                                                                                       aTCGCTGACAGCACTGTTATGGAAGGTG‑CGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGATTCAGCCTATTTAGACCGTTTCTTCGCCATTTaa                   <  1:473137/149‑1 (MQ=255)
                                                                                                                             ggcAGCACTGTTATGGAAGGCG‑CGAGTAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGtt             <  1:427068/147‑1 (MQ=255)
                                                                                                                                  cACTGTTATGGAAGGTG‑CGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATccc        >  1:107136/1‑149 (MQ=34)
                                                                                                                                     tGTTATGGAAGGTG‑CGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAACAGATTTTTTCTCCCGTAAATGCCTTGGATCCGACACTTTAGACCGTTTTGTCGCCATTAAAgagcgtattctcag     >  2:205333/1‑136 (MQ=11)
                                                                                                                                        tATGGAAGGTG‑CGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGttt  <  1:366696/149‑1 (MQ=32)
                                                                                                                                           |                                                                                                                                                  
CCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTG‑CGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTT  >  CP009273/269527‑269811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.