breseq  version 0.32.0b  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 273,525 T→C 17.5% E330G (GAG→GGG)  afuC ← CP4‑6 prophage; putative ferric transporter subunit
JC JC 542,271 IS5 (–) +4 bp 35.0% coding (136‑139/1668 nt) fdrA → putative NAD(P)‑binding acyl‑CoA synthetase
RA 821,679 C→T 18.4% G344R (GGG→AGG)  ybhS ← inner membrane putative ABC superfamily transporter permease
RA 1,191,676 C→T 58.7% H366H (CAC→CAT icd → e14 prophage; isocitrate dehydrogenase, specific for NADP+
RA 1,191,688 C→T 50.5% T370T (ACC→ACT icd → e14 prophage; isocitrate dehydrogenase, specific for NADP+
RA 1,191,701 T→C 41.9% L375L (TTA→CTA)  icd → e14 prophage; isocitrate dehydrogenase, specific for NADP+
RA 1,211,760 T→C 19.8% S79S (AGT→AGC ymgA → RcsB connector protein for regulation of biofilm
RA 1,268,411 C→T 17.4% A150V (GCC→GTC)  chaC → cation transport regulator
JC JC 2,400,257 IS5 (+) +4 bp 50.0% intergenic (‑137/‑780) lrhA ← / → alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2
JC 2,420,337 Δ120 bp 77.4% intergenic (‑70/+32) hisJ ← / ← argT histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit
RA 2,420,470 C→A 100% intergenic (‑203/+18) hisJ ← / ← argT histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit
RA 2,719,426 T→C 40.7% intergenic (‑321/+2) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
RA 2,725,094 A→G 21.0% G813G (GGT→GGC clpB ← protein disaggregation chaperone
JC JC 3,178,254 IS5 (+) +4 bp 80.0% coding (56‑59/291 nt) yqiC → BMFP family putative fusogenic protein
RA 3,180,810 G→A 16.8% pseudogene (31/2445 nt) yqiG → pseudogene; fimbrial export usher family;putative membrane; Not classified; putative membrane protein
RA 3,985,522 T→C 17.5% L337P (CTC→CCC)  cyaA → adenylate cyclase
RA 4,185,493 C→G 24.9% E179Q (GAG→CAG)  thiC ← phosphomethylpyrimidine synthase

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 360104 =NA (NA)30 (1.690) 22/276 NT NA intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 NA (NA)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 454243 =5 (0.280)6 (0.350) 6/270 NT 55.1% intergenic (+87/‑101) clpX/lon ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La
?CP009273 603467 = NA (NA)intergenic (+23/‑54) hokE/insL1 toxic polypeptide, small/IS186 transposase
* ? CP009273 = 4542555 (0.280)5 (0.290) 5/268 NT 50.7% intergenic (+99/‑89) clpX/lon ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La
?CP009273 = 604803 NA (NA)intergenic (+170/+112) insL1/entD IS186 transposase/phosphopantetheinyltransferase component of enterobactin synthase multienzyme complex
* ? CP009273 = 683479NA (NA)6 (0.350) 6/266 NT 36.1% noncoding (1023/1195 nt) IS5 repeat region
?CP009273 684510 = 11 (0.620)intergenic (‑41/+289) insH1/lnt IS5 transposase and trans‑activator/apolipoprotein N‑acyltransferase
* ? CP009273 = 1391495NA (NA)9 (0.510) 8/276 NT 52.9% intergenic (+146/‑127) insH1/ynaJ IS5 transposase and trans‑activator/DUF2534 family putative inner membrane protein
?CP009273 = 1972751 8 (0.450)intergenic (‑297/‑483) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
* ? CP009273 1972748 =9 (0.510)6 (0.340) 4/274 NT 40.0% intergenic (‑294/‑486) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
?CP009273 = 2283592 NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? CP009273 2269338 =14 (0.790)5 (0.280) 5/276 NT 26.3% coding (587/1026 nt) yejE microcin C transporter YejABEF, permease subunit; ABC family
?CP009273 = 3577555 NA (NA)intergenic (+15/‑1) insB1/yrhA IS1 transposase B/pseudogene, interrupted by IS1E
* ? CP009273 = 226934614 (0.790)13 (0.730) 11/276 NT 48.1% coding (595/1026 nt) yejE microcin C transporter YejABEF, permease subunit; ABC family
?CP009273 3576788 = NA (NA)intergenic (+10/‑55) yrhA/insA pseudogene, interrupted by IS1E/IS1 repressor TnpA
* ? CP009273 4532499 =8 (0.450)6 (0.360) 5/258 NT 42.1% intergenic (+49/‑433) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?CP009273 4532813 = 9 (0.540)intergenic (+363/‑119) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
* ? CP009273 = 45325077 (0.390)9 (0.540) 7/258 NT 53.7% intergenic (+57/‑425) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?CP009273 = 4532803 9 (0.540)intergenic (+353/‑129) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)