Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 2,601,785 Δ1 bp :: IS186 (–) +5 bp coding (585‑589/618 nt) hyfA → hydrogenase 4, 4Fe‑4S subunit

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 2514273 =NA (NA)25 (0.750) 23/240 0.6 100% noncoding (1/1345 nt) IS186 repeat region
?NC_000913 2601785 = 0 (0.000)coding (585/618 nt) hyfA hydrogenase 4, 4Fe‑4S subunit
* ? NC_000913 = 2515616NA (NA)23 (0.680) 22/242 0.7 100% noncoding (1344/1345 nt) IS186 repeat region
?NC_000913 = 2601789 0 (0.000)coding (589/618 nt) hyfA hydrogenase 4, 4Fe‑4S subunit

GAGCCCCGGCATTACGTGCCGAAGTATCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/2514421‑2514273
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gggaGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGAC  >  NC_000913/2601785‑2601901
                                                                                                                                                                                                                                                                          
GAGCCCCGGCATTACGTGCCGAAGTATCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGA                                                                                                                       <  4:150004/149‑1
 AGCCCCGGCATTACGTGCCGAAGTATCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGAG                                                                                                                      <  1:4771/149‑1
   CCCCGGCATTACGTGCCGAAGTATCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGG                                                                                                                         <  6:97246/144‑1
   CCCCGGCATTACGTGCCGAAGTATCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGG                                                                                                                         >  5:97246/1‑144
    CCCGGCATTACGTGCCGAAGTATCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGAGGAT                                                                                                                   >  4:67733/1‑149
               GTGCCGAAGTATCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGAGGATCCCCTTTCCCT                                                                                                        >  1:160009/1‑149
                 GCCGAAGTATCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGA                                                                                                                       <  1:190076/132‑1
                 GCCGAAGTATCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGA                                                                                                                       >  2:190076/1‑132
                          TCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGAGGATCCC                                                                                                                >  3:125663/1‑130
                          TCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGAGGATCCC                                                                                                                <  4:125663/130‑1
                          TCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGG                                                                                                                        >  2:46848/1‑122
                          TCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGG                                                                                                                        <  1:46848/122‑1
                                  TTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAA                                                                                     >  8:106633/1‑149
                                  TTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGG                                                                                                                         >  8:107235/1‑113
                                  TTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGG                                                                                                                         <  7:107235/113‑1
                                                        AGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAAC                                                               <  2:160009/149‑1
                                                        AGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAAC                                                               <  3:67733/149‑1
                                                                TGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGC                                                       >  3:7727/1‑149
                                                                     AGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATT                                                  <  7:106633/149‑1
                                                                             TGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCT                                          >  5:158188/1‑149
                                                                                   TAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGG                                                                                                                         >  6:19623/1‑64
                                                                                   TAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGG                                                                                                                         <  5:19623/64‑1
                                                                                                CGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGT                                        >  2:94944/1‑132
                                                                                                CGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGGCGCTGATTCTCTATCTGT                                        <  1:94944/132‑1
                                                                                                  TTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCT                     >  5:30573/1‑149
                                                                                                     AATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCT                                              <  1:116051/121‑1
                                                                                                     AATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCT                                              >  2:116051/1‑121
                                                                                                      ATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTT                 <  6:158188/149‑1
                                                                                                       TCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTT                >  5:169450/1‑149
                                                                                                          TATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGAGGATCCCCTTT                                                                                                            <  1:70885/54‑1
                                                                                                          TATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGAGGATCCCCTTT                                                                                                            >  2:70885/1‑54
                                                                                                                   TAATACCACAACCCTTAAGTTAGCGCTTATGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGC                                    >  6:169133/1‑117
                                                                                                                   TAATACCACAACCCTTAAGTTAGCGCTTATGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGC                                    <  5:169133/117‑1
                                                                                                                     ATACCACAACCCTTAAGTTAGCGCTTATGGGAGGATCACCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGAC  <  4:7727/149‑1
                                                                                                                                                TGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTAC             >  7:10228/1‑111
                                                                                                                                                TGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTAC             <  8:10228/111‑1
                                                                                                                                                                                                                                                                          
GAGCCCCGGCATTACGTGCCGAAGTATCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/2514421‑2514273
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gggaGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGAC  >  NC_000913/2601785‑2601901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.