| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| JC JC | NC_000913 | 2,601,785 | Δ1 bp :: IS186 (–) +5 bp | coding (585‑589/618 nt) | hyfA → | hydrogenase 4, 4Fe‑4S subunit |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913 | 2514273 = | NA (NA) | 25 (0.750) | 23/240 | 0.6 | 100% | noncoding (1/1345 nt) | IS186 | repeat region |
| ? | NC_000913 | 2601785 = | 0 (0.000) | coding (585/618 nt) | hyfA | hydrogenase 4, 4Fe‑4S subunit | |||||
| * | ? | NC_000913 | = 2515616 | NA (NA) | 23 (0.680) | 22/242 | 0.7 | 100% | noncoding (1344/1345 nt) | IS186 | repeat region |
| ? | NC_000913 | = 2601789 | 0 (0.000) | coding (589/618 nt) | hyfA | hydrogenase 4, 4Fe‑4S subunit | |||||
TGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTG > NC_000913/2601645‑2601907 | tgtgATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCccca < 4:113315‑M2/149‑5 (MQ=255) gtgccgaagtatccagttcttcgggcttaccaatatgggccagaattgctgaccagttatcgtgagagtaattcatcggcacgttaaatcatatcaggcgtaataccacaacccttaagttagcgcttatgggaGGATCCCCTTTCCct > 1:160009‑M2/135‑149 (MQ=255) tccagttcttcgggcttaccaatatgggccagaattgctgaccagttatcgtgagagtaattcatcggcacgttaaatcatatcaggcgtaataccacaacccttaagttagcgcttatgggaGGATccc < 4:125663‑M2/7‑1 (MQ=255) tccagttcttcgggcttaccaatatgggccagaattgctgaccagttatcgtgagagtaattcatcggcacgttaaatcatatcaggcgtaataccacaacccttaagttagcgcttatgggaGGATccc > 3:125663‑M2/124‑130 (MQ=255) ggTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCcccataagcgctaacttaaggg < 4:134105‑M2/149‑23 (MQ=255) gCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCcccataagcgctaacttaagggttgaac > 3:134105‑M2/1‑121 (MQ=255) ttcgggcttaccaatatgggccagaattgctgaccagttatcgtgagagtaattcatcggcacgttaaatcatatcaggcgtaataccacaacccttaagttagcgcttatgggaGGATCCCCTTTCCCTCACTCAGGAGCAACGCTaa > 8:106633‑M2/116‑149 (MQ=255) ccGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCcccataagcgctaacttaagggttgaaccat < 2:21801‑M2/134‑32 (MQ=255) ccGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCcccataagcgctaacttaagggttgaaccat > 1:21801‑M2/1‑103 (MQ=255) agaattgctgaccagttatcgtgagagtaattcatcggcacgttaaatcatatcaggcgtaataccacaacccttaagttagcgcttatgggaGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAAc < 2:160009‑M2/56‑1 (MQ=255) agaattgctgaccagttatcgtgagagtaattcatcggcacgttaaatcatatcaggcgtaataccacaacccttaagttagcgcttatgggaGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAAc < 3:67733‑M2/56‑1 (MQ=255) ggCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCcccataagcgctaacttaagggttgaaccatctgaagaatgcgacgcctcggtg < 7:124248‑M2/149‑55 (MQ=255) ggCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAAAGGCGGGGAGGATCcccataagcgctaacttaagggttgacccatctgaagaatgcgacgcctcggtg < 8:72640‑M2/149‑55 (MQ=255) tgaccagttatcgtgagagtaattcatcggcacgttaaatcatatcaggcgtaataccacaacccttaagttagcgcttatgggaGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGc > 3:7727‑M2/86‑149 (MQ=255) cGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCcccataagcgctaacttaag < 5:130464‑M2/108‑21 (MQ=255) cGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCcccataagcgctaacttaag > 6:130464‑M2/1‑88 (MQ=255) ggCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCcccataagcgctaacttaagggttgaaccatctgaagaatgcgacgcctcggtgcctcgtta > 3:113315‑M2/1‑87 (MQ=255) agttatcgtgagagtaattcatcggcacgttaaatcatatcaggcgtaataccacaacccttaagttagcgcttatgggaGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGAtt < 7:106633‑M2/69‑1 (MQ=255) aCCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCcccataagcgctaacttaagggttgaaccatctgaagaatgcgacgcctcggtgcctcgttaagacgatg < 3:19212‑M2/149‑71 (MQ=255) tgagagtaattcatcggcacgttaaatcatatcaggcgtaataccacaacccttaagttagcgcttatgggaGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCt > 5:158188‑M2/73‑149 (MQ=255) tGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCcccataa > 6:126363‑M2/1‑66 (MQ=255) tGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCcccataa < 5:126363‑M2/73‑8 (MQ=255) aaCGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCcccataagcgctaacttaagggttga > 4:16430‑M2/1‑63 (MQ=255) tCAGATGAAAGAAAATCAGCTCATTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCccaataagcgctaacttaagggttgaaccatctgaagaatgcga > 2:64288‑M2/1‑59 (MQ=255) tCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCcccataagcgctaacttaagggttgaaccatctgaagaatgcga < 1:64288‑M2/103‑45 (MQ=255) cgttaaatcatatcaggcgtaataccacaacccttaagttagcgcttatgggaGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGt > 2:94944‑M2/54‑132 (MQ=255) cgttaaatcatatcaggcgtaataccacaacccttaagttagcgcttatgggaGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGGCGCTGATTCTCTATCTGt < 1:94944‑M2/79‑1 (MQ=255) ttaaatcatatcaggcgtaataccacaacccttaagttagcgcttatgggaGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCt > 5:30573‑M2/52‑149 (MQ=255) aTGAAAGTAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCcccatattcgctaacttaagggtggaaccatctgaagaatgcgccgcctcggtgcctcgttaagacgatgcctcgcgttcttcaattgcgtttt > 7:72640‑M2/1‑55 (MQ=255) aatcatatcaggcgtaataccacaacccttaagttagcgcttatgggaGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTctct < 1:116051‑M2/73‑1 (MQ=255) aatcatatcaggcgtaataccacaacccttaagttagcgcttatgggaGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTctct > 2:116051‑M2/49‑121 (MQ=255) atcatatcaggcgtaataccacaacccttaagttagcgcttatgggaGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGttt < 6:158188‑M2/102‑1 (MQ=255) tcatatcaggcgtaataccacaacccttaagttagcgcttatgggaGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGtttt > 5:169450‑M2/47‑149 (MQ=255) tatcaggcgtaataccacaacccttaagttagcgcttatgggaGGATCCCCttt < 1:70885‑M2/11‑1 (MQ=255) tatcaggcgtaataccacaacccttaagttagcgcttatgggaGGATCCCCttt > 2:70885‑M2/44‑54 (MQ=255) aaaCAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCcccataagcgctaacttaagggttgaaccatctgaagaatgcgacgcctcggtgcctcgttaagacgatgcctcgcgttcttcaattgcgttttgtaggctgt < 2:4710‑M2/149‑104 (MQ=255) gcgcCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCcccataagcgctaacttaagggttgaaccatctgaagaatgcgacgcctcggtgcctcgttaagacgatgcctcgcgttcttcaattgcgttttgtaggctgtcaggg > 8:124248‑M2/1‑41 (MQ=255) taataccacaacccttaagttagcgcttatgggaGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGc < 5:169133‑M2/83‑1 (MQ=255) taataccacaacccttaagttagcgcttatgggaGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGc > 6:169133‑M2/35‑117 (MQ=255) ccTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCcccataagcgctaacttaagggttgaaccatctgaagaatgcgacgcctcggtgcctcgttaagacgatgcctcgcgttcttcaattgcgttttgtaggctgtcagggata > 4:19212‑M2/1‑38 (MQ=255) ataccacaacccttaagttagcgcttatgggaGGATCACCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGAc < 4:7727‑M2/117‑1 (MQ=255) gCAAGCTGGTTTGCCAATGGCGGGGAGGATCcccataagcgctaacttaagggttgaaccatctgaagaatgcgacgcctcggtgcctcgttaagacgatgcctcgcgttcttcaattgcgttttgtaggctgtcagggatactgtccc > 1:4710‑M2/1‑31 (MQ=255) gCAAGCTGGTTTGCCAATGGCGGGGAGGATCcccataagcgctaacttaagggttgaaccatctgaagaatgcgac > 5:81767‑M2/1‑31 (MQ=255) gCAAGCTGGTTTGCCAATGGCGGGGAGGATCcccataagcgctaacttaagggttgaaccatctgaagaatgcgac < 6:81767‑M2/76‑46 (MQ=255) tgggaGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTAc > 7:10228‑M2/6‑111 (MQ=255) tgggaGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTAc < 8:10228‑M2/106‑1 (MQ=255) tCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCt > 2:99821/1‑41 (MQ=255) tCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCt < 1:99821/41‑1 (MQ=255) | TGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTG > NC_000913/2601645‑2601907 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |