Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 2,601,785 Δ1 bp :: IS186 (–) +5 bp coding (585‑589/618 nt) hyfA → hydrogenase 4, 4Fe‑4S subunit

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 2514273 =NA (NA)25 (0.750) 23/240 0.6 100% noncoding (1/1345 nt) IS186 repeat region
?NC_000913 2601785 = 0 (0.000)coding (585/618 nt) hyfA hydrogenase 4, 4Fe‑4S subunit
* ? NC_000913 = 2515616NA (NA)23 (0.680) 22/242 0.7 100% noncoding (1344/1345 nt) IS186 repeat region
?NC_000913 = 2601789 0 (0.000)coding (589/618 nt) hyfA hydrogenase 4, 4Fe‑4S subunit

ATGGTCATCTGGAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2515466‑2515616
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gggGATCCTCCCCGCCATTGGCAAACCAGCTTGCGGCAAGGCGCTGTTTTTCTTTCATCTGACGTTGCAGGCTATCACCGGTGATCAGCCGTAACGCCTGATTCGGGCAAGCGCGAACGCAGGCCGGACCTTCTGGCAAGAAATCACA  <  NC_000913/2601789‑2601645
                                                                                                                                                                                                                                                                                                        
ATGGTCATCTGGAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATG                                                                                                                                                     <  3:57327/149‑1
                                 GGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGGATCCTCCCCGCCATTGGCAAACCAGCTTGC                                                                                                                    >  1:4710/1‑149
                                        TATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGGATCCTCCCCGCCATTGGCAAACCAGCTTGCGGCAAGG                                                                                                             >  4:19212/1‑149
                                           CCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGGATCCTCCCCGCCATTGGCAAACCAGCTTGCGGCAAGGCGC                                                                                                          >  8:124248/1‑149
                                             CTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGG                                                                                                                                                    <  7:123341/105‑1
                                             CTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGG                                                                                                                                                    >  8:123341/1‑105
                                              TGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGG                                                                                                                                                    >  8:29508/1‑104
                                              TGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGG                                                                                                                                                    <  7:29508/104‑1
                                                ACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGGATCCTCCCCGCCATTGGCAAACCAGCTTGCGGCAAGGCGCTGTTT                                                                                                     <  2:4710/149‑1
                                                         AAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGGCGCATTCTTCAGATGGTTCCACCCTTAAGTTAGCGAATATGGGGATCCTCCCCGCCATTGGCAAACCAGCTTGCGGCAAGGCGCTGTTTTACTTTCAT                                                                                            >  7:72640/1‑149
                                                                AATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGG                                                                                                                                                    <  2:6177/86‑1
                                                                AATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGG                                                                                                                                                    >  1:6177/1‑86
                                                                                 CATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGGATCCTCCCCGCCATTGGCAAACCAGCTTGCGGCAAGGCGCTGTTTTTCTTTCATCTGACGTTGCAGGCTATCACCGGT                                                                    <  3:19212/149‑1
                                                                                        TTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGG                                                                                                                                                    >  6:88140/1‑62
                                                                                        TTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGG                                                                                                                                                    <  5:88140/62‑1
                                                                                         TAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGGATCCTCCCCGCCATTGGCAAACCAGCTTGCGGCAAGGCGCTGTTTTTCTTTCATCTGACGTTGCAGGCTATCACCGGTGATCAGCC                                                            >  3:113315/1‑149
                                                                                                 CACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGGATCCTCCCCGCCATTGGCAAACCAGCTTGCGGCAAGGCGCTGTTTTTCTTTCATCTGACGTTGCAGGCTATCACCGGTGATCAGCCGTAACGCC                                                    <  7:124248/149‑1
                                                                                                 CACCGAGGCGTCGCATTCTTCAGATGGGTCAACCCTTAAGTTAGCGCTTATGGGGATCCTCCCCGCCTTTGGCAAACCAGCTTGCGGCAAGGCGCTGTTTTTCTTTCATCTGACGTTGCAGGCTATCACCGGTGATCAGCCGTAACGCC                                                    <  8:72640/149‑1
                                                                                                     GAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGG                                                                                                                                                    <  7:55591/49‑1
                                                                                                     GAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGG                                                                                                                                                    >  8:55591/1‑49
                                                                                                          GTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGGATCCTCCCCGCCATTGGCAAACCAGCTTGC                                                                                                                    >  5:81767/1‑76
                                                                                                          GTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGGATCCTCCCCGCCATTGGCAAACCAGCTTGC                                                                                                                    <  6:81767/76‑1
                                                                                                           TCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATTGGGATCCTCCCCGCCATTGGCAAACCAGCTTGCGGCAATGAGCTGATTTTCTTTCATCTGA                                                                                        >  2:64288/1‑103
                                                                                                           TCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGGATCCTCCCCGCCATTGGCAAACCAGCTTGCGGCAAGGCGCTGTTTTTCTTTCATCTGA                                                                                        <  1:64288/103‑1
                                                                                                                        ATGGTTCAACCCTTAAGTTAGCGCTTATGGGGATCCTCCCCGCCATTGGCAAACCAGCTTGCGGCAAGGCGCTGTTTTTCTTTCATCTGACGTTGCAGGCTATCACCGGTGATCAGCCGTAACGCCTGATTCGG                                            <  2:21801/134‑1
                                                                                                                        ATGGTTCAACCCTTAAGTTAGCGCTTATGGGGATCCTCCCCGCCATTGGCAAACCAGCTTGCGGCAAGGCGCTGTTTTTCTTTCATCTGACGTTGCAGGCTATCACCGGTGATCAGCCGTAACGCCTGATTCGG                                            >  1:21801/1‑134
                                                                                                                           GTTCAACCCTTAAGTTAGCGCTTATGGGGATCCTCCCCGCCATTGGCAAACCAGCTTGCGGCAAGGCGCTGTTTTTCTTTCATCTGACGTTGCAGGCTATCACCGGTGATCAGCCGTAACGCCTGATTCGGGCAAGCGCGAACGCAGGC                          >  3:134105/1‑149
                                                                                                                             TCAACCCTTAAGTTAGCGCTTATGGGGATCCTCCCCGCCATTGGCAAACCAGCTTGCGGCAAGGCGCTGTTTTTCTTTCATCTGACGTT                                                                                    >  4:16430/1‑89
                                                                                                                                 CCCTTAAGTTAGCGCTTATGGGGATCCTCCCCGCCATTGGCAAACCAGCTTGCGGCAAGGCGCTGTTTTTCTTTCATCTGACGTTGCAGGCTATCACCGGTGATCAGCCGTAACGCCTGATTCGGGCAAGCGCGAACGCAGGCCGGACC                    <  4:134105/149‑1
                                                                                                                                   CTTAAGTTAGCGCTTATGGGGATCCTCCCCGCCATTGGCAAACCAGCTTGCGGCAAGGCGCTGTTTTTCTTTCATCTGACGTTGCAGGCTATCACCGGTGATCAGCCG                                                           <  5:130464/108‑1
                                                                                                                                   CTTAAGTTAGCGCTTATGGGGATCCTCCCCGCCATTGGCAAACCAGCTTGCGGCAAGGCGCTGTTTTTCTTTCATCTGACGTTGCAGGCTATCACCGGTGATCAGCCG                                                           >  6:130464/1‑108
                                                                                                                                                TTATGGGGATCCTCCCCGCCATTGGCAAACCAGCTTGCGGCAAGGCGCTGTTTTTCTTTCATCTGACGTTGCA                                                                                 >  6:126363/1‑73
                                                                                                                                                TTATGGGGATCCTCCCCGCCATTGGCAAACCAGCTTGCGGCAAGGCGCTGTTTTTCTTTCATCTGACGTTGCA                                                                                 <  5:126363/73‑1
                                                                                                                                                   TGGGGATCCTCCCCGCCATTGGCAAACCAGCTTGCGGCAAGGCGCTGTTTTTCTTTCATCTGACGTTGCAGGCTATCACCGGTGATCAGCCGTAACGCCTGATTCGGGCAAGCGCGAACGCAGGCCGGACCTTCTGGCAAGAAATCACA  <  4:113315/149‑1
                                                                                                                                                                                                                                                                                                        
ATGGTCATCTGGAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2515466‑2515616
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gggGATCCTCCCCGCCATTGGCAAACCAGCTTGCGGCAAGGCGCTGTTTTTCTTTCATCTGACGTTGCAGGCTATCACCGGTGATCAGCCGTAACGCCTGATTCGGGCAAGCGCGAACGCAGGCCGGACCTTCTGGCAAGAAATCACA  <  NC_000913/2601789‑2601645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.