Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 3,976,196 Δ9 bp coding (1052‑1060/1131 nt) wecE → TDP‑4‑oxo‑6‑deoxy‑D‑glucose transaminase

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3976196 3976204 9 45 [0] [0] 46 wecE TDP‑4‑oxo‑6‑deoxy‑D‑glucose transaminase

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 39761950 (0.000)43 (1.090) 34/266 0.1 100% coding (1051/1131 nt) wecE TDP‑4‑oxo‑6‑deoxy‑D‑glucose transaminase
?NC_000913 3976205 = 0 (0.000)coding (1061/1131 nt) wecE TDP‑4‑oxo‑6‑deoxy‑D‑glucose transaminase

TAACTTTCTGAAAGAAGCGGAAATCATGGCGGTGTTTCATTACATTCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCTGCGCCTGCCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGT  >  NC_000913/3976065‑3976347
                                                                                                                                            |                                                                                                                                              
cgcgtggattaactttctgaaaggagcggaaatcatggcggtgtttcattacattccgctgcagggttgcgctgcgggtgaacacttgggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGc                                                                                                                                              <  1:314292‑M2/3‑1 (MQ=255)
        ttaactttctgaaagaagcggaaatcatggcggtgtttcattacattccgctgcacggttgccctgcgggggaacactttggtgagttccacggtgaagatcgctacacaaccaaagagagcgagcgccggcCGCGGTTCTAc                                                                                                                                      >  2:386184‑M2/133‑143 (MQ=255)
          gccttcccgcaagaagcggaaaccatgtcggtggtgcattacattccgctgcacggttgccctgcgggggaacactttggtgagttccaccgtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACaa                                                                                                                                    <  2:148709‑M2/13‑1 (MQ=255)
             tttctgaaagaagcggaaatcatggcggtgtttcattacattccgctgcacggttgccctgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAaccc                                                                                                                                 >  1:712423‑M2/128‑142 (MQ=255)
                         gcggaaatcatggcggtgtttcattacattccgctgcacggttgccctgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCCGTTCTACAACCTGTCGCCCGTCaa                                                                                                                     >  1:273690‑M2/116‑143 (MQ=255)
                         gcggaaatcatggcggtgtttcattacattccgctgcacggttgccctgcgggggaacactttggtgagttccacggtgaagatcgctacacaaccgacgcaagtgtgctcctgcCGCTGTCCTACAACCTGTCGCCCGCcag                                                                                                                     >  1:150837‑M2/116‑142 (MQ=255)
                           ggaaatcatggcggtgtttcattacattccgctgcacggttgccctgcgggggaacactttggtgagttccacggtggagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACACCCTGTCGCCCTTCACTc                                                                                                                   >  2:63149‑M2/114‑143 (MQ=255)
                           ggaaatcatggcggtgtttcattacattccgctgcacggttgccctgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCCCTGTTCTACAACCTGTCGCCCGTAAata                                                                                                                   >  2:404151‑M2/114‑142 (MQ=255)
                              gatcggggctgtgttgcagtctattccggcgctcggttggcgtgcgggggaacactttggtgagttccacggtgaagatcgctacgccaccaacgagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGc                                                                                                                <  1:390286‑M2/33‑1 (MQ=255)
                              aatcatggcggtgtttcattacattctgctgcacgtagtccctgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGc                                                                                                                <  2:691241‑M2/33‑1 (MQ=255)
                                       ggtgtttctttacattccgctgcacggttgcccttggggggaacactttggtgagttccactgtgaagatcgctccacccccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTaa                                                                                                       <  1:241490‑M2/42‑1 (MQ=255)
                                          gtttcattacattccgctgcacggttgccctgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagcgagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGAAAGGAAATTg                                                                                                    >  2:1768‑M2/99‑143 (MQ=255)
                                                  acattccgctgcacggttgccctgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATGAGCGTACGGTAATTGCGACTttg                                                                                            >  2:594873‑M2/91‑143 (MQ=255)
                                                   cattccgctgcacggttgccctgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgcgcgcctgcCGCTGTTCTACAACCTGTCGCCCGCCAATCAGCGGACGGTAATTGCGACTttgt                                                                                           >  2:368196‑M2/90‑143 (MQ=255)
                                                    attccgctgcacggttgccctgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTttgtt                                                                                          <  1:659580‑M2/55‑1 (MQ=255)
                                                    actccgctgcacggttgtcccccgggggaacactttggtgagttccacagtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTttgtt                                                                                          <  2:20882‑M2/55‑1 (MQ=255)
                                                    actccgcggcacggtggccccgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTttgtt                                                                                          <  2:632370‑M2/55‑1 (MQ=255)
                                                      tccgctgcacggttgccctgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGa                                                                                        >  1:665219‑M2/87‑143 (MQ=255)
                                                          ctgcacggtctcccggcgggggaccactttggcgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAacta                                                                                    <  2:210921‑M2/61‑1 (MQ=255)
                                                              acggttgccctgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAGTCAGCGGACGGTCATTGCGACTTTGTTGAAGTActtg                                                                                >  2:150144‑M2/79‑142 (MQ=255)
                                                                ggttgccctgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTc                                                                              <  1:605788‑M2/67‑1 (MQ=255)
                                                                     ccgtgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGat                                                                         <  1:505786‑M2/72‑1 (MQ=255)
                                                                      cctgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagcgagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGGCAGCGCGacgttgtcgaactcctgtggctgcaa                                                                        >  2:180536‑M2/71‑133 (MQ=255)
                                                                      cctgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTGCGGTGATTGTGCCTTCGGTGACGTAGTGTTCCGGata                                                                        >  2:60694‑M2/71‑143 (MQ=255)
                                                                      cctgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGata                                                                        >  2:157081‑M2/71‑143 (MQ=255)
                                                                      cctgcgggggaacactttggtgagttccacggtgaagaccgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGata                                                                        <  2:229232‑M2/73‑1 (MQ=255)
                                                                        tgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAGTTGCGACTGTGTTGACCTACTTTTCCTGATATg                                                                      >  2:516166‑M2/69‑143 (MQ=255)
                                                                                   actttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCaaa                                                           <  2:457630‑M2/86‑1 (MQ=255)
                                                                                    ctttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCaaaa                                                          <  1:157081‑M2/87‑1 (MQ=255)
                                                                                    ctttcgtgagttgcacggtgaagctccctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGGAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCaaaa                                                          <  2:296318‑M2/87‑1 (MQ=255)
                                                                                     tttggtgggttccacggtgaagttcgctacctcaccaacgagagcttcttcctgcCGCTGTTCTACAACCTGTCGCTCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAg                                                         <  1:632143‑M2/88‑1 (MQ=255)
                                                                                               tccacggggaagcccgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTtgtg                                               <  1:167580‑M2/98‑1 (MQ=255)
                                                                                                   cggtgaggatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGAcg                                           <  1:460970‑M2/102‑1 (MQ=255)
                                                                                                    ggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGGCCTTGTGGCcgg                                          >  1:475010‑M2/41‑143 (MQ=255)
                                                                                                        aagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACggcggc                                      <  2:122716‑M2/107‑1 (MQ=255)
                                                                                                        aagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGGAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGCGCTCGTGGacggcgga                                      >  2:375363‑M2/37‑142 (MQ=255)
                                                                                                             cgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTa                                 <  2:297039‑M2/112‑1 (MQ=255)
                                                                                                                    ccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTAGTGGCCGTCGGCCGGTACACTGGt                          >  1:545528‑M2/25‑143 (MQ=255)
                                                                                                                         aaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGGTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGa                     >  2:519730‑M2/20‑143 (MQ=255)
                                                                                                                           agagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGAtt                   <  2:127078‑M2/126‑1 (MQ=255)
                                                                                                                             agagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTCCTTTTCCTGTTATGTCGTTTGCAAAAGCGTACTTGTGAAAGGAGACCAGTACAATGGTCAAGATTgg                 >  2:5306‑M2/16‑143 (MQ=255)
                                                                                                                              gagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGt                <  2:280941‑M2/129‑1 (MQ=255)
                                                                                                                                  gagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCg            <  1:579808‑M2/133‑1 (MQ=255)
                                                                                                                                    gcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCggg          <  2:610936‑M2/135‑1 (MQ=255)
                                                                                                                                           cCGCTGTTCTACAACCTGTCGCCCGTCTATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTgg   <  1:6077‑M2/142‑1 (MQ=255)
                                                                                                                                            cGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGACAAAGTGGACGGCGGCAAGTACGCGGCTCAAGATTGGTGCCGGGTTACTgca  >  1:7093/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                              
TAACTTTCTGAAAGAAGCGGAAATCATGGCGGTGTTTCATTACATTCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCTGCGCCTGCCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGT  >  NC_000913/3976065‑3976347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.