Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	3,976,196	Δ9 bp	coding (1052‑1060/1131 nt)	wecE →	TDP‑4‑oxo‑6‑deoxy‑D‑glucose transaminase

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	3976196	3976204	9	45 [0]	[0] 46	wecE	TDP‑4‑oxo‑6‑deoxy‑D‑glucose transaminase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3976195	0 (0.000)	43 (1.090)	34/266	0.1	100%	coding (1051/1131 nt)	wecE	TDP‑4‑oxo‑6‑deoxy‑D‑glucose transaminase
	?	NC_000913	3976205 =	0 (0.000)					coding (1061/1131 nt)	wecE	TDP‑4‑oxo‑6‑deoxy‑D‑glucose transaminase

TAACTTTCTGAAAGAAGCGGAAATCATGGCGGTGTTTCATTACATTCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCTGCGCCTGCCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGT  >  NC_000913/3976065‑3976347                                                                                                                                             |                                                                                                                                               cgcgtggattaactttctgaaaggagcggaaatcatggcggtgtttcattacattccgctgcagggttgcgctgcgggtgaacacttgggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGc                                                                                                                                              <  1:314292‑M2/3‑1 (MQ=255)         ttaactttctgaaagaagcggaaatcatggcggtgtttcattacattccgctgcacggttgccctgcgggggaacactttggtgagttccacggtgaagatcgctacacaaccaaagagagcgagcgccggcCGCGGTTCTAc                                                                                                                                      >  2:386184‑M2/133‑143 (MQ=255)           gccttcccgcaagaagcggaaaccatgtcggtggtgcattacattccgctgcacggttgccctgcgggggaacactttggtgagttccaccgtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACaa                                                                                                                                    <  2:148709‑M2/13‑1 (MQ=255)              tttctgaaagaagcggaaatcatggcggtgtttcattacattccgctgcacggttgccctgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAaccc                                                                                                                                 >  1:712423‑M2/128‑142 (MQ=255)                          gcggaaatcatggcggtgtttcattacattccgctgcacggttgccctgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCCGTTCTACAACCTGTCGCCCGTCaa                                                                                                                     >  1:273690‑M2/116‑143 (MQ=255)                          gcggaaatcatggcggtgtttcattacattccgctgcacggttgccctgcgggggaacactttggtgagttccacggtgaagatcgctacacaaccgacgcaagtgtgctcctgcCGCTGTCCTACAACCTGTCGCCCGCcag                                                                                                                     >  1:150837‑M2/116‑142 (MQ=255)                            ggaaatcatggcggtgtttcattacattccgctgcacggttgccctgcgggggaacactttggtgagttccacggtggagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACACCCTGTCGCCCTTCACTc                                                                                                                   >  2:63149‑M2/114‑143 (MQ=255)                            ggaaatcatggcggtgtttcattacattccgctgcacggttgccctgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCCCTGTTCTACAACCTGTCGCCCGTAAata                                                                                                                   >  2:404151‑M2/114‑142 (MQ=255)                               gatcggggctgtgttgcagtctattccggcgctcggttggcgtgcgggggaacactttggtgagttccacggtgaagatcgctacgccaccaacgagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGc                                                                                                                <  1:390286‑M2/33‑1 (MQ=255)                               aatcatggcggtgtttcattacattctgctgcacgtagtccctgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGc                                                                                                                <  2:691241‑M2/33‑1 (MQ=255)                                        ggtgtttctttacattccgctgcacggttgcccttggggggaacactttggtgagttccactgtgaagatcgctccacccccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTaa                                                                                                       <  1:241490‑M2/42‑1 (MQ=255)                                           gtttcattacattccgctgcacggttgccctgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagcgagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGAAAGGAAATTg                                                                                                    >  2:1768‑M2/99‑143 (MQ=255)                                                   acattccgctgcacggttgccctgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATGAGCGTACGGTAATTGCGACTttg                                                                                            >  2:594873‑M2/91‑143 (MQ=255)                                                    cattccgctgcacggttgccctgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgcgcgcctgcCGCTGTTCTACAACCTGTCGCCCGCCAATCAGCGGACGGTAATTGCGACTttgt                                                                                           >  2:368196‑M2/90‑143 (MQ=255)                                                     attccgctgcacggttgccctgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTttgtt                                                                                          <  1:659580‑M2/55‑1 (MQ=255)                                                     actccgctgcacggttgtcccccgggggaacactttggtgagttccacagtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTttgtt                                                                                          <  2:20882‑M2/55‑1 (MQ=255)                                                     actccgcggcacggtggccccgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTttgtt                                                                                          <  2:632370‑M2/55‑1 (MQ=255)                                                       tccgctgcacggttgccctgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGa                                                                                        >  1:665219‑M2/87‑143 (MQ=255)                                                           ctgcacggtctcccggcgggggaccactttggcgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAacta                                                                                    <  2:210921‑M2/61‑1 (MQ=255)                                                               acggttgccctgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAGTCAGCGGACGGTCATTGCGACTTTGTTGAAGTActtg                                                                                >  2:150144‑M2/79‑142 (MQ=255)                                                                 ggttgccctgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTc                                                                              <  1:605788‑M2/67‑1 (MQ=255)                                                                      ccgtgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGat                                                                         <  1:505786‑M2/72‑1 (MQ=255)                                                                       cctgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagcgagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGGCAGCGCGacgttgtcgaactcctgtggctgcaa                                                                        >  2:180536‑M2/71‑133 (MQ=255)                                                                       cctgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTGCGGTGATTGTGCCTTCGGTGACGTAGTGTTCCGGata                                                                        >  2:60694‑M2/71‑143 (MQ=255)                                                                       cctgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGata                                                                        >  2:157081‑M2/71‑143 (MQ=255)                                                                       cctgcgggggaacactttggtgagttccacggtgaagaccgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGata                                                                        <  2:229232‑M2/73‑1 (MQ=255)                                                                         tgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAGTTGCGACTGTGTTGACCTACTTTTCCTGATATg                                                                      >  2:516166‑M2/69‑143 (MQ=255)                                                                                    actttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCaaa                                                           <  2:457630‑M2/86‑1 (MQ=255)                                                                                     ctttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCaaaa                                                          <  1:157081‑M2/87‑1 (MQ=255)                                                                                     ctttcgtgagttgcacggtgaagctccctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGGAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCaaaa                                                          <  2:296318‑M2/87‑1 (MQ=255)                                                                                      tttggtgggttccacggtgaagttcgctacctcaccaacgagagcttcttcctgcCGCTGTTCTACAACCTGTCGCTCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAg                                                         <  1:632143‑M2/88‑1 (MQ=255)                                                                                                tccacggggaagcccgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTtgtg                                               <  1:167580‑M2/98‑1 (MQ=255)                                                                                                    cggtgaggatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGAcg                                           <  1:460970‑M2/102‑1 (MQ=255)                                                                                                     ggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGGCCTTGTGGCcgg                                          >  1:475010‑M2/41‑143 (MQ=255)                                                                                                         aagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACggcggc                                      <  2:122716‑M2/107‑1 (MQ=255)                                                                                                         aagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGGAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGCGCTCGTGGacggcgga                                      >  2:375363‑M2/37‑142 (MQ=255)                                                                                                              cgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTa                                 <  2:297039‑M2/112‑1 (MQ=255)                                                                                                                     ccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTAGTGGCCGTCGGCCGGTACACTGGt                          >  1:545528‑M2/25‑143 (MQ=255)                                                                                                                          aaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGGTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGa                     >  2:519730‑M2/20‑143 (MQ=255)                                                                                                                            agagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGAtt                   <  2:127078‑M2/126‑1 (MQ=255)                                                                                                                              agagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTCCTTTTCCTGTTATGTCGTTTGCAAAAGCGTACTTGTGAAAGGAGACCAGTACAATGGTCAAGATTgg                 >  2:5306‑M2/16‑143 (MQ=255)                                                                                                                               gagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGt                <  2:280941‑M2/129‑1 (MQ=255)                                                                                                                                   gagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCg            <  1:579808‑M2/133‑1 (MQ=255)                                                                                                                                     gcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCggg          <  2:610936‑M2/135‑1 (MQ=255)                                                                                                                                            cCGCTGTTCTACAACCTGTCGCCCGTCTATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTgg   <  1:6077‑M2/142‑1 (MQ=255)                                                                                                                                             cGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGACAAAGTGGACGGCGGCAAGTACGCGGCTCAAGATTGGTGCCGGGTTACTgca  >  1:7093/1‑141 (MQ=255)                                                                                                                                             |                                                                                                                                               TAACTTTCTGAAAGAAGCGGAAATCATGGCGGTGTTTCATTACATTCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCTGCGCCTGCCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGT  >  NC_000913/3976065‑3976347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑