Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	3,976,196	Δ9 bp	coding (1052‑1060/1131 nt)	wecE →	TDP‑4‑oxo‑6‑deoxy‑D‑glucose transaminase

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	3976196	3976204	9	45 [0]	[0] 46	wecE	TDP‑4‑oxo‑6‑deoxy‑D‑glucose transaminase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3976195	0 (0.000)	43 (1.090)	34/266	0.1	100%	coding (1051/1131 nt)	wecE	TDP‑4‑oxo‑6‑deoxy‑D‑glucose transaminase
	?	NC_000913	3976205 =	0 (0.000)					coding (1061/1131 nt)	wecE	TDP‑4‑oxo‑6‑deoxy‑D‑glucose transaminase

CGCGTTGATTAACTTTCTGAAAGAAGCGGAAATCATGGCGGTGTTTCATTACATTCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCTGCGCCTGCCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCG  >  NC_000913/3976056‑3976337                                                                                                                                            |                                                                                                                                               cgcgTGGATTAACTTTCTGAAAGGAGCGGAAATCATGGCGGTGTTTCATTACATTCCGCTGCAGGGTTGCGCTGCGGGTGAACACTTGGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgc                                                                                                                                             <  1:314292‑M1/143‑4 (MQ=255)         ttAACTTTCTGAAAGAAGCGGAAATCATGGCGGTGTTTCATTACATTCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACAACCAAAGAGAGCGAGCGCCGGCcgcggttctac                                                                                                                                     >  2:386184‑M1/1‑132 (MQ=255)           gccttcccgcaagaagcggaaaccatGTCGGTGGTGCATTACATTCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACCGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaa                                                                                                                                   <  2:148709‑M1/133‑14 (MQ=255)              tttCTGAAAGAAGCGGAAATCATGGCGGTGTTTCATTACATTCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaaccc                                                                                                                                >  1:712423‑M1/1‑127 (MQ=255)                          gCGGAAATCATGGCGGTGTTTCATTACATTCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgccgttctacaacctgtcgcccgtcaa                                                                                                                    >  1:273690‑M1/1‑115 (MQ=255)                          gCGGAAATCATGGCGGTGTTTCATTACATTCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACAACCGACGCAAGTGTGCTCCTGCcgctgtcctacaacctgtcgcccgccag                                                                                                                    >  1:150837‑M1/1‑115 (MQ=255)                            ggAAATCATGGCGGTGTTTCATTACATTCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGGAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaccctgtcgcccttcactc                                                                                                                  >  2:63149‑M1/1‑113 (MQ=255)                            ggAAATCATGGCGGTGTTTCATTACATTCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCccctgttctacaacctgtcgcccgtaaata                                                                                                                  >  2:404151‑M1/1‑113 (MQ=255)                               gatcggggctgtgtTGCAGTCTATTCCGGCGCTCGGTTGGCGTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACGCCACCAACGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagc                                                                                                               <  1:390286‑M1/137‑34 (MQ=255)                               aaTCATGGCGGTGTTTCATTACATTCTGCTGCACGTAGTCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagc                                                                                                               <  2:691241‑M1/143‑34 (MQ=255)                                        ggTGTTTCTTTACATTCCGCTGCACGGTTGCCCTTGGGGGGAACACTTTGGTGAGTTCCACTGTGAAGATCGCTCCACCCCCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaa                                                                                                      <  1:241490‑M1/143‑43 (MQ=255)                                           gtTTCATTACATTCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGCGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgaaaggaaattg                                                                                                   >  2:1768‑M1/1‑98 (MQ=255)                                                   aCATTCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatgagcgtacggtaattgcgactttg                                                                                           >  2:594873‑M1/1‑90 (MQ=255)                                                    cATTCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGCGCGCCTGCcgctgttctacaacctgtcgcccgccaatcagcggacggtaattgcgactttgt                                                                                          >  2:368196‑M1/1‑89 (MQ=255)                                                     actccGCTGCACGGTTGTCCCCCGGGGGAACACTTTGGTGAGTTCCACAGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgtt                                                                                         <  2:20882‑M1/141‑56 (MQ=255)                                                     actccGCGGCACGGTGGCCCCGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgtt                                                                                         <  2:632370‑M1/141‑56 (MQ=255)                                                     aTTCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgtt                                                                                         <  1:659580‑M1/143‑56 (MQ=255)                                                       tCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgttga                                                                                       >  1:665219‑M1/1‑86 (MQ=255)                                                           cTGCACGGTCTCCCGGCGGGGGACCACTTTGGCGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgttgaacta                                                                                   <  2:210921‑M1/143‑62 (MQ=255)                                                               aCGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcagtcagcggacggtcattgcgactttgttgaagtacttg                                                                               >  2:150144‑M1/1‑78 (MQ=255)                                                                 ggTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgttgaactacttttc                                                                             <  1:605788‑M1/143‑68 (MQ=255)                                                                      ccgtgcgGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgttgaactacttttcctgat                                                                        <  1:505786‑M1/140‑73 (MQ=255)                                                                       ccTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGCGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacgggcagcgcgacgttgtcgaactcctgtggctgcaa                                                                       >  2:180536‑M1/1‑70 (MQ=255)                                                                       ccTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtgcggtgattgtgccttcggtgacgtagtgttccggata                                                                       >  2:60694‑M1/1‑70 (MQ=255)                                                                       ccTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgttgaactacttttcctgata                                                                       >  2:157081‑M1/1‑70 (MQ=255)                                                                       ccTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGACCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgttgaactacttttcctgata                                                                       <  2:229232‑M1/143‑74 (MQ=255)                                                                         tGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtagttgcgactgtgttgacctacttttcctgatatg                                                                     >  2:516166‑M1/1‑68 (MQ=255)                                                                                    acTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgttgaactacttttcctgatatgtcgttggcaaa                                                          <  2:457630‑M1/143‑87 (MQ=255)                                                                                     cTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgttgaactacttttcctgatatgtcgttggcaaaa                                                         <  1:157081‑M1/143‑88 (MQ=255)                                                                                     cTTTCGTGAGTTGCACGGTGAAGCTCCCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacgggaattgcgactttgttgaactacttttcctgatatgtcgttggcaaaa                                                         <  2:296318‑M1/143‑88 (MQ=255)                                                                                      tttGGTGGGTTCCACGGTGAAGTTCGCTACCTCACCAACGAGAGCTTCTTCCTGCcgctgttctacaacctgtcgctcgtcaatcagcgtacggtaattgcgactttgttgaactacttttcctgatatgtcgttggcaaaag                                                        <  1:632143‑M1/143‑89 (MQ=255)                                                                                                tCCACGGGGAAGCCCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgttgaactacttttcctgatatgtcgttggcaaaagcgtccttgtg                                              <  1:167580‑M1/143‑99 (MQ=255)                                                                                                    cGGTGAGGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgttgaactacttttcctgatatgtcgttggcaaaagcgtccttgtggacg                                          <  1:460970‑M1/143‑103 (MQ=255)                                                                                                     ggTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgttgaactacttttcctgatatgtcgttggcaaaagcggccttgtggccgg                                         >  1:475010‑M1/1‑40 (MQ=255)                                                                                                         aaGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgttgaactacttttcctgatatgtcgttggcaaaagcgtccttgtggacggcggc                                     <  2:122716‑M1/143‑108 (MQ=255)                                                                                                         aaGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacgggaattgcgactttgttgaactacttttcctgatatgtcgttggcaaaagcgcgctcgtggacggcgga                                     >  2:375363‑M1/1‑36 (MQ=255)                                                                                                              cGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgttgaactacttttcctgatatgtcgttggcaaaagcgtccttgtggacggcggccagta                                <  2:297039‑M1/143‑113 (MQ=255)                                                                                                                     ccaccaAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgttgaactacttttcctgatatgtcgttggcaaaagcgtcctagtggccgtcggccggtacactggt                         >  1:545528‑M1/1‑24 (MQ=255)                                                                                                                          aaaGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttggtgaactacttttcctgatatgtcgttggcaaaagcgtccttgtggacggcggccagtacactggtcaaga                    >  2:519730‑M1/1‑19 (MQ=255)                                                                                                                            agagagCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgttgaactacttttcctgatatgtcgttggcaaaagcgtccttgtggacggcggccagtacactggtcaagatt                  <  2:127078‑M1/143‑127 (MQ=255)                                                                                                                              agagCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgttgaactccttttcctgttatgtcgtttgcaaaagcgtacttgtgaaaggagaccagtacaatggtcaagattgg                >  2:5306‑M1/1‑15 (MQ=255)                                                                                                                               gagcgagcGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgttgaactacttttcctgatatgtcgttggcaaaagcgtccttgtggacggcggccagtacactggtcaagattggt               <  2:280941‑M1/143‑130 (MQ=255)                                                                                                                                   gagcgCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgttgaactacttttcctgatatgtcgttggcaaaagcgtccttgtggacggcggccagtacactggtcaagattggtgccg           <  1:579808‑M1/143‑134 (MQ=255)                                                                                                                                     gcgcCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgttgaactacttttcctgatatgtcgttggcaaaagcgtccttgtggacggcggccagtacactggtcaagattggtgccggg         <  2:610936‑M1/143‑136 (MQ=255)                                                                                                                                            ccgctgttctacaacctgtcgcccgtctatcagcgtacggtaattgcgactttgttgaactacttttcctgatatgtcgttggcaaaagcgtccttgtggacggcggccagtacactggtcaagattggtgccgggttactgg  <  1:6077‑M1/143‑143 (MQ=255)                                                                                                                                            |                                                                                                                                               CGCGTTGATTAACTTTCTGAAAGAAGCGGAAATCATGGCGGTGTTTCATTACATTCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCTGCGCCTGCCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCG  >  NC_000913/3976056‑3976337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑