breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 26 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,0600CT37.0% 26.8 / 23.3 27intergenic (+266/+376)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
*NC_0009133,959,5150TA33.3% 26.4 / 21.3 27intergenic (+70/+17)ilvC/ppiCketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
*NC_0009131,912,7480TG29.2% 31.7 / 14.6 24intergenic (‑172/+20)htpX/prcputative endopeptidase/carboxy‑terminal protease for penicillin‑binding protein 3
*NC_0009131,912,7490TA29.2% 30.9 / 15.4 24intergenic (‑173/+19)htpX/prcputative endopeptidase/carboxy‑terminal protease for penicillin‑binding protein 3
*NC_0009131,912,7500TA29.2% 32.2 / 15.6 24intergenic (‑174/+18)htpX/prcputative endopeptidase/carboxy‑terminal protease for penicillin‑binding protein 3
*NC_0009131,388,2840AT28.9% 52.0 / 25.6 39intergenic (+23/+21)tyrR/tpxaromatic amino acid biosynthesis and transport regulon transcriptional regulator; autorepressor; ATPase; phosphatase/lipid hydroperoxide peroxidase
*NC_0009131,912,7510CA26.9% 42.9 / 16.2 26intergenic (‑175/+17)htpX/prcputative endopeptidase/carboxy‑terminal protease for penicillin‑binding protein 3
*NC_0009131,388,2830CT25.6% 64.8 / 20.4 39intergenic (+22/+22)tyrR/tpxaromatic amino acid biosynthesis and transport regulon transcriptional regulator; autorepressor; ATPase; phosphatase/lipid hydroperoxide peroxidase
*NC_0009131,388,2850AG25.6% 59.1 / 21.9 39intergenic (+24/+20)tyrR/tpxaromatic amino acid biosynthesis and transport regulon transcriptional regulator; autorepressor; ATPase; phosphatase/lipid hydroperoxide peroxidase
*NC_0009132,121,5881.C24.1% 60.0 / 28.6 29intergenic (‑34/+21)wcaJ/cpsGcolanic biosynthesis UDP‑glucose lipid carrier transferase/phosphomannomutase
*NC_0009132,657,4260GA23.1% 46.0 / 10.4 26L504L (CTG→TTG) hscADnaK‑like molecular chaperone specific for IscU
*NC_0009132,121,5830G.22.6% 78.8 / 20.8 31intergenic (‑29/+26)wcaJ/cpsGcolanic biosynthesis UDP‑glucose lipid carrier transferase/phosphomannomutase
*NC_0009132,121,5860GC22.6% 63.0 / 18.0 31intergenic (‑32/+23)wcaJ/cpsGcolanic biosynthesis UDP‑glucose lipid carrier transferase/phosphomannomutase
*NC_0009133,891,5020CG21.6% 72.4 / 20.9 37intergenic (+19/‑113)tnaB/mdtLtryptophan transporter of low affinity/multidrug efflux system protein
*NC_0009133,368,2190GA21.4% 52.3 / 10.2 28T131T (ACG→ACAyhcGDUF1016 family protein in the PD‑(D/E)XK nuclease superfamily
*NC_0009133,368,2200CG21.4% 60.4 / 14.8 28L132V (CTG→GTG) yhcGDUF1016 family protein in the PD‑(D/E)XK nuclease superfamily
*NC_0009132,657,4320AC20.7% 52.7 / 14.2 29Y502D (TAC→GAC) hscADnaK‑like molecular chaperone specific for IscU
*NC_0009132,657,4330AT20.7% 50.0 / 11.5 29S501S (TCT→TCAhscADnaK‑like molecular chaperone specific for IscU
*NC_0009132,657,4340GT20.7% 69.0 / 11.9 29S501Y (TCT→TAT) hscADnaK‑like molecular chaperone specific for IscU
*NC_0009133,368,2210TC20.7% 54.8 / 10.1 29L132P (CTG→CCG) yhcGDUF1016 family protein in the PD‑(D/E)XK nuclease superfamily

Marginal new junction evidence (lowest skew 10 of 40 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 68488326 (0.840)6 (0.200) 5/252 3.2 19.2% coding (373/726 nt) gltL glutamate/aspartate ABC transporter ATPase
?NC_000913 = 684903 26 (0.880)coding (353/726 nt) gltL glutamate/aspartate ABC transporter ATPase
* ? NC_000913 = 434179537 (1.190)4 (0.150) 4/230 3.3 10.7% intergenic (‑167/‑116) melR/melA melibiose operon transcriptional regulator; autoregulator/alpha‑galactosidase, NAD(P)‑binding
?NC_000913 = 4341815 35 (1.310)intergenic (‑187/‑96) melR/melA melibiose operon transcriptional regulator; autoregulator/alpha‑galactosidase, NAD(P)‑binding
* ? NC_000913 = 461079226 (0.840)4 (0.140) 4/248 3.5 16.9% coding (1379/1590 nt) prfC peptide chain release factor RF‑3
?NC_000913 = 4610809 15 (0.520)coding (1396/1590 nt) prfC peptide chain release factor RF‑3
* ? NC_000913 = 424170137 (1.190)4 (0.140) 4/252 3.5 9.9% coding (554/1476 nt) xylE D‑xylose transporter
?NC_000913 = 4241729 38 (1.290)coding (526/1476 nt) xylE D‑xylose transporter
* ? NC_000913 = 397301837 (1.190)4 (0.130) 4/254 3.5 11.5% coding (497/1068 nt) rffG dTDP‑glucose 4,6‑dehydratase
?NC_000913 = 3973039 26 (0.880)coding (518/1068 nt) rffG dTDP‑glucose 4,6‑dehydratase
* ? NC_000913 = 378380531 (1.000)4 (0.140) 4/250 3.5 10.7% coding (324/468 nt) secB protein export chaperone
?NC_000913 = 3783825 38 (1.300)coding (304/468 nt) secB protein export chaperone
* ? NC_000913 3235543 =28 (0.900)4 (0.140) 4/252 3.5 13.0% coding (333/1137 nt) rlmG 23S rRNA m(2)G1835 methyltransferase, SAM‑dependent
?NC_000913 3235583 = 27 (0.920)coding (293/1137 nt) rlmG 23S rRNA m(2)G1835 methyltransferase, SAM‑dependent
* ? NC_000913 = 313455537 (1.190)6 (0.200) 4/254 3.5 13.4% coding (425/693 nt) yghT putative ATP‑binding protein
?NC_000913 = 3134592 42 (1.420)coding (462/693 nt) yghT putative ATP‑binding protein
* ? NC_000913 1510110 =37 (1.190)5 (0.170) 4/252 3.5 13.0% coding (108/1407 nt) ydcR putative DNA‑binding transcriptional regulator and putative aminotransferase
?NC_000913 1510142 = 32 (1.090)coding (140/1407 nt) ydcR putative DNA‑binding transcriptional regulator and putative aminotransferase
* ? NC_000913 1179565 =15 (0.480)4 (0.130) 4/254 3.5 21.4% intergenic (+61/‑66) nagK/cobB N‑acetyl‑D‑glucosamine kinase/deacetylase of acs and cheY, chemotaxis regulator
?NC_000913 1179588 = 15 (0.510)intergenic (+84/‑43) nagK/cobB N‑acetyl‑D‑glucosamine kinase/deacetylase of acs and cheY, chemotaxis regulator