breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp [crl] [crl]
RA 1,662,350 C→T P599S (CCC→TCC)  ynfF → S‑ and N‑oxide reductase, A subunit, periplasmic
RA 1,806,340 C→T intergenic (‑257/‑30) ydiY ← / → pfkB acid‑inducible putative outer membrane protein/6‑phosphofructokinase II
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
JC JC 3,560,930 IS5 (+) +4 bp coding (520‑523/831 nt) glpG ← rhomboid intramembrane serine protease
RA 4,182,881 A→C E546A (GAA→GCA)  rpoB → RNA polymerase, beta subunit
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 2185834 2185861 28 6 [4] [4] 6 rcnR/rcnA transcriptional repressor of rcnA/membrane protein conferring nickel and cobalt resistance
* * ÷ NC_000913 3423751–3424234 3424549–3424238 5–799 5 [4] [3] 5 [rrfD]–[rrlD] [rrfD],[rrlD]
* * ÷ NC_000913 4107528 4108498 971 6 [4] [3] 5 [pfkA] [pfkA]
* * ÷ NC_000913 4144055 4147087 3033 6 [1] [4] 6 [pflD]–[pflC] [pflD],[pflC]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 224374NA (NA)4 (0.150) 4/252 3.0 NA noncoding (604/1542 nt) rrsH 16S ribosomal RNA of rrnH operon
?NC_000913 = 224403 NA (NA)noncoding (633/1542 nt) rrsH 16S ribosomal RNA of rrnH operon
* ? NC_000913 = 271191NA (NA)6 (0.230) 4/254 3.0 NA noncoding (651/1221 nt) IS30 repeat region
?NC_000913 = 271214 NA (NA)noncoding (674/1221 nt) IS30 repeat region
* ? NC_000913 = 28289020 (0.720)6 (0.220) 5/260 2.7 21.6% coding (613/909 nt) yagE 2‑keto‑3‑deoxy gluconate (KDG) aldolase; CP4‑6 prophage
?NC_000913 = 282923 24 (0.880)coding (646/909 nt) yagE 2‑keto‑3‑deoxy gluconate (KDG) aldolase; CP4‑6 prophage
* ? NC_000913 715247 =NA (NA)4 (0.150)
+CAGTCTCA
4/250 2.9 NA intergenic (+49/‑165) pgm/ybfP phosphoglucomutase/lipoprotein
?NC_000913 = 3012605 NA (NA)intergenic (+212/+9) yqeA/yqeB putative amino acid kinase/XdhC‑CoxI family protein with NAD(P)‑binding Rossman fold
* ? NC_000913 1207790 =32 (1.150)8 (0.330) 8/234 1.7 27.5% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 14 (0.570)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)26 (0.970) 26/258 0.2 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 452479919 (0.680)4 (0.160) 4/246 2.9 20.2% coding (212/906 nt) yjhH putative lyase/synthase
?NC_000913 = 4524824 14 (0.550)coding (187/906 nt) yjhH putative lyase/synthase