Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	1,299,499	Δ1,199 bp	intergenic (+254/‑485)	ychE → / → oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1299499	1300697–1299500	2–1199	63 [0]	[0] 64	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1299498	0 (0.000)	60 (1.190)	49/274	0.1	100%	intergenic (+253/‑1684)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
	?	NC_000913	1300698 =	0 (0.000)					intergenic (+1453/‑484)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

AATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTC  >  NC_000913/1299357‑1299638                                                                                                                                              |                                                                                                                                             aaTTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAa                                                                                                                                             >  2:598730‑M1/1‑142 (MQ=255)         ttagttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatc                                                                                                                                     >  2:720621‑M1/1‑134 (MQ=255)         ttagttaTAAGGATTGCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTACTTCCACTGCCTTTGGTAGAGAAAAGTGCTAAataatcatc                                                                                                                                     >  2:889953‑M1/1‑134 (MQ=255)          tagttaTAAGGTGTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatca                                                                                                                                    <  2:800580‑M1/143‑11 (MQ=255)           cgttatAATGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaa                                                                                                                                   <  1:736192‑M1/142‑12 (MQ=255)            gttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaat                                                                                                                                  <  2:312309‑M1/143‑13 (MQ=255)             tcacaaggatTTCCTTAACTGCTCCTCCTCACCAGCATGTTATTGTCCCCACATCATAATCCTTTGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaatt                                                                                                                                 <  1:219683‑M1/139‑14 (MQ=255)                   ggATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaa                                                                                                                           >  2:181335‑M1/1‑124 (MQ=255)                       ttCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACCTCCTAATCCTGGGCTTCCTGCAGACTAATTCAAATGATATTATTAATTCCACCGCCTTCGGTAGAGGCAAGTGCTAAatattaatcaattgttatattat                                                                                                                       >  2:884664‑M1/1‑120 (MQ=255)                        tCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattatt                                                                                                                      <  1:734588‑M1/143‑25 (MQ=255)                          cTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgt                                                                                                                    >  1:319231‑M1/1‑117 (MQ=255)                           ttAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtg                                                                                                                   <  2:73313‑M1/143‑28 (MQ=255)                              aCTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttacattattgtgcat                                                                                                                >  2:637623‑M1/1‑113 (MQ=255)                                  cTTCTCCTCATCATCATTTTATTTTCGTCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCTACTGTCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttca                                                                                                            <  2:399501‑M1/143‑35 (MQ=255)                                        ctcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactg                                                                                                      <  1:612682‑M1/143‑41 (MQ=255)                                         tcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattatgcatttcactactga                                                                                                     >  2:302369‑M1/1‑102 (MQ=255)                                         tcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGATAGAGGAAAGTACTAAataagaataaattgttaagttattgtcgctttcacatgtag                                                                                                     >  2:616038‑M1/1‑102 (MQ=255)                                                  tGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaat                                                                                            >  1:586146‑M1/1‑93 (MQ=255)                                                    ttATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGGATTAGGTACAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatca                                                                                          >  1:700789‑M1/1‑91 (MQ=255)                                                    ttATTTTCGCCACATCATAATCCTGGGCTTGCTGAAAAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactgaaacagtaatca                                                                                          >  2:277840‑M1/1‑91 (MQ=255)                                                     tATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcag                                                                                         <  1:91296‑M1/143‑54 (MQ=255)                                                       ttttCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATCTCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaa                                                                                       <  1:950112‑M1/143‑56 (MQ=255)                                                        tttCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaa                                                                                      <  2:640246‑M1/143‑57 (MQ=255)                                                         ttggatacatcataaTCCTTTGCTTGATGAAGAATAATTTAAATTATATTATTAATTCCACTGTCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgtatttcactactggaactgtaatcagaaaa                                                                                     <  2:905367‑M1/137‑58 (MQ=255)                                                             ccACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagata                                                                                 >  1:147239‑M1/1‑82 (MQ=255)                                                              cacaTCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatccattgttaaaatattgtgcatttcactactcgagctgtcatcagaaaacagag                                                                                >  2:890161‑M1/1‑81 (MQ=255)                                                                    atAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgc                                                                          >  2:761720‑M1/1‑75 (MQ=255)                                                                    atAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGGAGAGGACTGTGTCCAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgc                                                                          >  1:701065‑M1/1‑75 (MQ=255)                                                                               gttgCAGCAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatct                                                               <  2:615403‑M1/142‑80 (MQ=255)                                                                                ttgttgAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGTAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactcgaactgtaatcagaaaagatagacatgcttagccaatctc                                                              <  2:173197‑M1/143‑81 (MQ=255)                                                                                 tGCTGAAGAATAATTGAAACGAGATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctct                                                             <  1:351551‑M1/143‑82 (MQ=255)                                                                                   cTGAAGAATAATTCAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcctttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctat                                                           <  1:63567‑M1/143‑84 (MQ=255)                                                                                      aagaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttg                                                        <  1:347878‑M1/143‑87 (MQ=255)                                                                                         aataatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgatt                                                     <  2:586146‑M1/143‑90 (MQ=255)                                                                                         aataatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatatgatt                                                     <  1:579627‑M1/143‑90 (MQ=255)                                                                                             atTGAAATGATATTATTAATTCCACTTGCTTTGGTTGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggacctgtaatcagaaaagatagacatgcttagccaatctctatttgattgaat                                                 <  2:908534‑M1/143‑94 (MQ=255)                                                                                               tGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttaggcaatctctatttgattgaattg                                               >  2:806791‑M1/1‑48 (MQ=255)                                                                                                  aaggataTTCGTAATTCCACTGGCTTTGGTACAGGAAAGTGCTAAacaagaatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaa                                            <  1:595277‑M1/140‑99 (MQ=255)                                                                                                  aaTGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaa                                            <  1:587484‑M1/143‑99 (MQ=255)                                                                                                     gATATTATTCACTCCACTGCCGTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagat                                         <  1:847549‑M1/143‑102 (MQ=255)                                                                                                        tttattaaTTCCCATCCCTTTTGTAGAGGAAAGTCCTAAataacaataaattgataaattattgtgcatgtgactacttgaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtt                                      <  2:336978‑M1/142‑105 (MQ=255)                                                                                                          tattaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttg                                    >  1:699319‑M1/1‑37 (MQ=255)                                                                                                            ttaATTGCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagaaatgcttagccaatctctatttgattgaattgaaagatgtctgtg                                  >  2:174068‑M1/1‑35 (MQ=255)                                                                                                            ttaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaccttattttgcatttaactaatggaactgtaatcagaaaagatagacatgcttagacaatatttatttgattgaattgcaagatgtttgtt                                  >  1:367130‑M1/1‑35 (MQ=255)                                                                                                            ttaAGTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgtt                                  <  1:838259‑M1/143‑109 (MQ=255)                                                                                                               aTTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaag                               <  1:191951‑M1/143‑112 (MQ=255)                                                                                                                 tCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttggttgaattgaaagatgtttgttaaggc                             >  2:36826‑M1/1‑30 (MQ=255)                                                                                                                     cggccTTTGGGGGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatgg                         <  1:207710‑M1/141‑118 (MQ=255)                                                                                                                     cTGCCATTGGTAGAGGAAAGTGCTAAataataaacaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatgg                         <  1:70235‑M1/143‑118 (MQ=255)                                                                                                                      tGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatgga                        >  1:98890‑M1/1‑25 (MQ=255)                                                                                                                          tttGGTAAAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctattggattgaattgaaagatgttagataaggcatgcatgca                    >  2:529043‑M1/1‑21 (MQ=255)                                                                                                                            tGGGAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaag                  <  2:349199‑M1/143‑125 (MQ=255)                                                                                                                              gTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaacgatgtttgttaagccatggatgcaagct                >  2:327442‑M1/1‑17 (MQ=255)                                                                                                                              gTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgttggttaaggcatgtatgcaagcc                >  2:67322‑M1/1‑17 (MQ=255)                                                                                                                               tAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagcta               >  1:426195‑M1/1‑16 (MQ=255)                                                                                                                               tAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagcta               >  1:705008‑M1/1‑16 (MQ=255)                                                                                                                                  agGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatag            >  1:677098‑M1/1‑13 (MQ=255)                                                                                                                                      tagTGCTAAataatactcaattgttaaattattgtggatttcactactggaactgtaatcagaaaagatagacatgtttagccaatctgtatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattc        <  2:815888‑M1/142‑135 (MQ=255)                                                                                                                                      aattgctAAataataatcaattgttaaattattgtgcatttcactagtggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattc        <  2:885441‑M1/140‑135 (MQ=255)                                                                                                                                       aGTGCTAAataataatcaattgttagattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattct       >  1:774383‑M1/1‑8 (MQ=255)                                                                                                                                           cTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgata   <  1:46520‑M1/143‑140 (MQ=255)                                                                                                                                           cTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgata   <  1:720621‑M1/143‑140 (MQ=255)                                                                                                                                            tAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctaattgcttgaattgaaagaagtttgttaaggcacggatgcaagaaataaatactgatac  >  2:665294‑M1/1‑3 (MQ=255)                                                                                                                                              |                                                                                                                                             AATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTC  >  NC_000913/1299357‑1299638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑