Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	1,299,499	Δ1,199 bp	intergenic (+254/‑485)	ychE → / → oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1299499	1300697–1299500	2–1199	63 [0]	[0] 64	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1299498	0 (0.000)	60 (1.190)	49/274	0.1	100%	intergenic (+253/‑1684)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
	?	NC_000913	1300698 =	0 (0.000)					intergenic (+1453/‑484)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

GATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGT  >  NC_000913/1300556‑1300840                                                                                                                                               |                                                                                                                                               aattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaa                                                                                                                                                >  2:598730‑M2/143‑143 (MQ=255)         ttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATc                                                                                                                                        >  2:720621‑M2/135‑143 (MQ=255)         ttagttataaggattgccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattacttccactgcctttggtagagaaaagtgctaaATAatcatc                                                                                                                                        >  2:889953‑M2/135‑143 (MQ=255)          tagttataaggtgttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCa                                                                                                                                       <  2:800580‑M2/10‑1 (MQ=255)           cgttataatgatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCaa                                                                                                                                      <  1:736192‑M2/11‑1 (MQ=255)            gttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAAt                                                                                                                                     <  2:312309‑M2/12‑1 (MQ=255)             tcacaaggatttccttaactgctcctcctcaccagcatgttattgtccccacatcataatcctttgcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAAtt                                                                                                                                    <  1:219683‑M2/13‑1 (MQ=255)                   ggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTaaa                                                                                                                              >  2:181335‑M2/125‑143 (MQ=255)                       ttccttaactgcttctcctcaccatcatgttattttcgccacctcctaatcctgggcttcctgcagactaattcaaatgatattattaattccaccgccttcggtagaggcaagtgctaaATATTAATCAATTGTTAtattat                                                                                                                          >  2:884664‑M2/121‑143 (MQ=255)                        tccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAattatt                                                                                                                         <  1:734588‑M2/24‑1 (MQ=255)                          cttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATtgt                                                                                                                       >  1:319231‑M2/118‑143 (MQ=255)                           ttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATtgtg                                                                                                                      <  2:73313‑M2/27‑1 (MQ=255)                              actgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTACATTATTGTGCAt                                                                                                                   >  2:637623‑M2/114‑143 (MQ=255)                                  cttctcctcatcatcattttattttcgtcacatcataatcctgggcttgctgaagaataattgaaatgatattattaattctactgtctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCa                                                                                                               <  2:399501‑M2/34‑1 (MQ=255)                                        ctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTg                                                                                                         <  1:612682‑M2/40‑1 (MQ=255)                                         tcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTATGCATTTCACTACtga                                                                                                        >  2:302369‑M2/103‑142 (MQ=255)                                         tcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttgatagaggaaagtactaaATAAGAATAAATTGTTAAGTTATTGTCgctttcacatgtag                                                                                                        >  2:616038‑M2/103‑137 (MQ=255)                                                  tgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAAt                                                                                               >  1:586146‑M2/94‑143 (MQ=255)                                                    ttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactggattaggtacaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCa                                                                                             >  1:700789‑M2/92‑143 (MQ=255)                                                    ttattttcgccacatcataatcctgggcttgctgaaaaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGAAACAGTAATCa                                                                                             >  2:277840‑M2/92‑143 (MQ=255)                                                     tattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAg                                                                                            <  1:91296‑M2/53‑1 (MQ=255)                                                       ttttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaatctcactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaa                                                                                          <  1:950112‑M2/55‑1 (MQ=255)                                                        tttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaaa                                                                                         <  2:640246‑M2/56‑1 (MQ=255)                                                         ttggatacatcataatcctttgcttgatgaagaataatttaaattatattattaattccactgtctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGTATTTCACTACTGGAACTGTAATCAGaaaa                                                                                        <  2:905367‑M2/57‑1 (MQ=255)                                                             ccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATa                                                                                    >  1:147239‑M2/83‑143 (MQ=255)                                                              cacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCCATTGTTAAAATATTGTGCATTTCACTACTCGAGCTGTCatcagaaaacagag                                                                                   >  2:890161‑M2/82‑138 (MQ=255)                                                                    ataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGc                                                                             >  2:761720‑M2/76‑143 (MQ=255)                                                                    ataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttgggagaggactgtgtccaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGc                                                                             >  1:701065‑M2/76‑143 (MQ=255)                                                                               gttgcagcagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAAtct                                                                  <  2:615403‑M2/79‑1 (MQ=255)                                                                                ttgttgaagaataattgaaatgatattattaattccactgcctttggtagaggtaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTCGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAAtctc                                                                 <  2:173197‑M2/80‑1 (MQ=255)                                                                                 tgctgaagaataattgaaacgagattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATctct                                                                <  1:351551‑M2/81‑1 (MQ=255)                                                                                   ctgaagaataattcaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCCTTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTAt                                                              <  1:63567‑M2/83‑1 (MQ=255)                                                                                      aagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttg                                                           <  1:347878‑M2/86‑1 (MQ=255)                                                                                         aataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgatt                                                        <  2:586146‑M2/89‑1 (MQ=255)                                                                                         aataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATatgatt                                                        <  1:579627‑M2/89‑1 (MQ=255)                                                                                             attgaaatgatattattaattccacttgctttggttgaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGACCTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaat                                                    <  2:908534‑M2/93‑1 (MQ=255)                                                                                               tgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGGCAATCTCTATTTGattgaattg                                                  >  2:806791‑M2/49‑143 (MQ=255)                                                                                                  aatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGaaa                                               <  1:587484‑M2/98‑1 (MQ=255)                                                                                                  aaggatattcgtaattccactggctttggtacaggaaagtgctaaACAAGAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGaaa                                               <  1:595277‑M2/98‑1 (MQ=255)                                                                                                     gatattattcactccactgccgttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAt                                            <  1:847549‑M2/101‑1 (MQ=255)                                                                                                        tttattaattcccatcccttttgtagaggaaagtcctaaATAACAATAAATTGATAAATTATTGTGCATGTGACTACTTGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtt                                         <  2:336978‑M2/104‑1 (MQ=255)                                                                                                          tattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtttg                                       >  1:699319‑M2/38‑143 (MQ=255)                                                                                                            ttaattgcactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGAAATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGatgtctgtg                                     >  2:174068‑M2/36‑142 (MQ=255)                                                                                                            ttaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTACCTTATTTTGCATTTAACTAATGGAACTGTAATCAGAAAAGATAGACATGCTTAGACAATATTTATTTGATTGAATTGCAAGAtgtttgtt                                     >  1:367130‑M2/36‑143 (MQ=255)                                                                                                            ttaagtccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtttgtt                                     <  1:838259‑M2/108‑1 (MQ=255)                                                                                                               attccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAg                                  <  1:191951‑M2/111‑1 (MQ=255)                                                                                                                 tccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGGTTGAATTGAAAGATGTTTGTTAAGGc                                >  2:36826‑M2/31‑143 (MQ=255)                                                                                                                     ctgccattggtagaggaaagtgctaaATAATAAACAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATgg                            <  1:70235‑M2/117‑1 (MQ=255)                                                                                                                     cggcctttgggggaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATgg                            <  1:207710‑M2/117‑1 (MQ=255)                                                                                                                      tgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGa                           >  1:98890‑M2/26‑143 (MQ=255)                                                                                                                          tttggtaaaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTGGATTGAATTGAAAGATGTTAGATAAGGCAtgcatgca                       >  2:529043‑M2/22‑143 (MQ=255)                                                                                                                            tgggagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAg                     <  2:349199‑M2/124‑1 (MQ=255)                                                                                                                              gtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAACGATGTTTGTTAAGCCATGGATGCAAGCt                   >  2:327442‑M2/18‑143 (MQ=255)                                                                                                                              gtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTGGTTAAGGCATGTATGCAAgcc                   >  2:67322‑M2/18‑142 (MQ=255)                                                                                                                               tagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCta                  >  1:705008‑M2/17‑143 (MQ=255)                                                                                                                               tagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCta                  >  1:426195‑M2/17‑143 (MQ=255)                                                                                                                                  aggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAg               >  1:677098‑M2/14‑143 (MQ=255)                                                                                                                                      tagtgctaaATAATACTCAATTGTTAAATTATTGTGGATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGTTTAGCCAATCTGTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTc           <  2:815888‑M2/134‑1 (MQ=255)                                                                                                                                      aattgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTAGTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTc           <  2:885441‑M2/134‑1 (MQ=255)                                                                                                                                       agtgctaaATAATAATCAATTGTTAGATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCt          >  1:774383‑M2/9‑143 (MQ=255)                                                                                                                                           ctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATa      <  1:720621‑M2/139‑1 (MQ=255)                                                                                                                                           ctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATa      <  1:46520‑M2/139‑1 (MQ=255)                                                                                                                                            taaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTAATTGCTTGAATTGAAAGAAGTTTGTTAAGGCACGGATGCAAGAAATAAATACTGATAc     >  2:665294‑M2/4‑143 (MQ=255)                                                                                                                                               ataataatCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGt  <  2:701065/143‑1 (MQ=255)                                                                                                                                               |                                                                                                                                               GATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGT  >  NC_000913/1300556‑1300840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑