Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,876,678	IS5 (+) +4 bp	coding (98‑101/1038 nt)	iap →	aminopeptidase in alkaline phosphatase isozyme conversion

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2288919 =	NA (NA)	32 (0.860)	31/284	0.2	100%	noncoding (1195/1195 nt)	IS5	repeat region
	?	NC_000913	2876678 =	0 (0.000)					coding (98/1038 nt)	iap	aminopeptidase in alkaline phosphatase isozyme conversion
*	?	NC_000913	= 2290113	NA (NA)	29 (0.780)	28/284	0.3	100%	noncoding (1/1195 nt)	IS5	repeat region
	?	NC_000913	= 2876681	0 (0.000)					coding (101/1038 nt)	iap	aminopeptidase in alkaline phosphatase isozyme conversion

CTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTAT‑GGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCC  >  NC_000913/2289980‑2290246                                                                                                                                       |                                                                                                                                      ctctTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACACTGTTCTAT‑GGCTACAGATGACAAACATGATATCATACCAGGGACTTGATCGCACCTTCCACAACgata                                                                                                                              >  2:486753/1‑140 (MQ=11)   ctTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTAT‑GGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTa                                                                                                                            <  1:227557/143‑1 (MQ=38)    ttttCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTAT‑GGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAg                                                                                                                           >  1:447780/1‑143 (MQ=38)        cTGGTTTGACGGCGCTTTCTGCTGAATTCCCTGTCGGCGAAGGTAAGTTGATGACTCATGATTAACCCTGTTCTAT‑GGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGccac                                                                                                                       <  2:374601/143‑1 (MQ=255)         tGGTCTGACGGCGCTTCCTGCTGACTTCACTGTCGGCGCAGGTAAGTTGATGACTCATGATGAACCCTGTTCTAT‑TGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGccacc                                                                                                                      <  2:29483/143‑1 (MQ=255)           gTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTAT‑GGCTCCAGATGGCAAACATGATCTCATATCAGGGACTTGTTCGCACCACCCAAAACGCTGTAGCCACCAg                                                                                                                    >  1:599156/1‑143 (MQ=255)                     gcTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTAT‑GGCTCCAGATGACAAACATGATCTCATATCCGGGACTTCTCCGCACCTCCCAAAACGCTGTAGCCACCAGAACCGATAtt                                                                                                          >  2:312157/1‑143 (MQ=17)                               gAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTG‑TCTATCCGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGAc                                                                                                <  1:489825/143‑1 (MQ=255)                                   tCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTAT‑GGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCCCCAGAACAGATATTGCGGAACGACAAag                                                                                            >  2:374228/1‑143 (MQ=255)                                       tGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTAT‑GGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGaa                                                                                        >  1:333840/1‑143 (MQ=255)                                        gTCGGCGAAGGTAAGTTGATGCCTCATGATGAACCCTGTTCTAT‑GGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCCTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGaaa                                                                                       <  1:199204/143‑1 (MQ=255)                                         tCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTAT‑GGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCGCCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGaaaa                                                                                      >  1:601316/1‑142 (MQ=255)                                           ggCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTAT‑GGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGTACGACACAGAGAAACAg                                                                                    >  1:552766/1‑143 (MQ=255)                                            gCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTAT‑GGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGa                                                                                   <  1:359163/143‑1 (MQ=255)                                            gCGAAGGTAAGTTGATGACTCATGATGAACCCTGCTCTAT‑GGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGCGCGACAAAGCGAAACAGa                                                                                   >  1:143335/1‑143 (MQ=255)                                             cGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTAT‑GGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGaa                                                                                  <  2:56172/143‑1 (MQ=255)                                              gAAGGTAAGTTGATGACTCATGAAGAACCCTGTTCTAT‑GGCTACAGATGACAAACATGATCACATATCAGGGACTTGTTCACACCTTACATAACGCTGTAGCCACCAGAACAGAAATTGCGAAAAAACAAAGAGAAACAAAAc                                                                                 >  2:211847/1‑143 (MQ=18)                                                     aGTTGATGACTCATGATGAACCCTGTTCTAT‑GGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAAGGACAAAGCGAAACAGAACCAGATTg                                                                          >  1:211743/1‑143 (MQ=255)                                                     aGTGGATGACGCATGATGAACCCTGGTCTAT‑GGCTCCAGATGACAAACAGGATCTCATAGCAGGGACTTGTCCGCATCTTCCATAACGCGGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTg                                                                          <  2:442597/143‑1 (MQ=25)                                                       ttGATGACTCATGAGGAACCCTGTTCTAT‑GGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGAt                                                                        <  1:221179/143‑1 (MQ=255)                                                         gatgaCTCATGATGAACCCTGTTCTAT‑GGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGACTGATGc                                                                      >  2:117747/1‑143 (MQ=255)                                                          atgaCTCATGATGAACCCTGTTCTAT‑GGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCCCCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCa                                                                     >  1:25300/1‑143 (MQ=255)                                                               tCATGATGAACCCTGTTCTAT‑GGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAg                                                                >  2:4187/1‑143 (MQ=255)                                                                       gaCCCTGTTCTAT‑CGCTCCATATTACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATc                                                        <  2:46851/142‑1 (MQ=255)                                                                         cccTGTTCTAT‑GGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCt                                                      >  1:360538/1‑143 (MQ=255)                                                                          ccTGTTCTAT‑GGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGGAACAGAACCAGATTGATGCATTGAGCTTTCATCCTa                                                     <  1:434805/143‑1 (MQ=255)                                                                          ccTGTTCTAT‑GGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTa                                                     >  2:283320/1‑143 (MQ=255)                                                                           cttttCTAT‑TGTTTCAGATGACTTATTTGATCTCATTTCAGGGACTTGTTCGTACCTTCTATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGCGCTTTTCTCCttt                                                    <  2:332230/140‑3 (MQ=255)                                                                            tGTTCTAT‑GGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGACCGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATg                                                   >  1:511363/1‑143 (MQ=255)                                                                                     ggCTCCAGATGACAAACATGATCTCATATCAGTGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAaattaat                                           <  2:380968/143‑1 (MQ=255)                                                                                         ccAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCt                                       <  2:18612/143‑1 (MQ=255)                                                                                            gATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGtt                                    >  1:502926/1‑143 (MQ=255)                                                                                                     cATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCAtt                           >  1:345608/1‑143 (MQ=255)                                                                                                       tGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTgg                         <  1:582390/143‑1 (MQ=255)                                                                                                        gATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTggg                        <  1:500143/143‑1 (MQ=255)                                                                                                        caTCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTggg                        <  1:292553/142‑1 (MQ=255)                                                                                                          tctcATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGGAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTa                      >  2:372417/1‑143 (MQ=255)                                                                                                              atatCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGa                  >  1:67477/1‑143 (MQ=255)                                                                                                               tatCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGaa                 >  2:594394/1‑143 (MQ=255)                                                                                                                atCAGGGACTTCTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGaaa                <  2:393136/143‑1 (MQ=255)                                                                                                                 tCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCGCCAGAACAGATATTGCGGAACGACAAAGAGGAGCAGACCCAGATTGATGCATTGAGCTTTCATCCTATGAAATTGATTGCTGTTAAGAGGCTTGATTACAGaaag               >  1:209387/1‑142 (MQ=255)                                                                                                                   acagACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATa             <  2:50979/140‑1 (MQ=255)                                                                                                                   aGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATa             <  2:595057/143‑1 (MQ=255)                                                                                                                     cgatttGTGCGCACCTGCAAGAAGCCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATAcc           <  1:360182/139‑1 (MQ=255)                                                                                                                              cGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTcc  >  2:471799/1‑143 (MQ=255)                                                                                                                                       |                                                                                                                                      CTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTAT‑GGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCC  >  NC_000913/2289980‑2290246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.