Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	1,299,499	Δ1,199 bp	intergenic (+254/‑485)	ychE → / → oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1299499–1300693	1300697	5–1199	85 [0]	[0] 86	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1299498	0 (0.000)	81 (1.160)	65/276	0.0	100%	intergenic (+253/‑1684)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
	?	NC_000913	1300698 =	0 (0.000)					intergenic (+1453/‑484)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

TAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGT  >  NC_000913/1300558‑1300840                                                                                                                                             |                                                                                                                                               ttacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctttgtttgctgaagaataattgaaatgatattattaattccactgcctgtggtagaggaaagtgctaaata                                                                                                                                              <  2:454641‑M2/3‑1 (MQ=255)  ttacagttagttataaggatttccttaactgcttatcctcaccatcatgttattttcgccacattataatcctcggcttgctcaagaataattgaaatgatattcttaattacactgcccttggaagaggaaagtctaaaata                                                                                                                                             >  1:65870‑M2/140‑140 (MQ=255)    cagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataata                                                                                                                                           <  1:466250‑M2/6‑1 (MQ=255)     agttagttataaggatttccttaactgcttctcctcaccttcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataataa                                                                                                                                          <  1:341512‑M2/7‑1 (MQ=255)     agttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcatactcatgagcttccgccaggatcattgacatgatattattcagtccactgcctttggtagaggaaagtgctaaataatag                                                                                                                                          >  1:441885‑M2/137‑142 (MQ=255)     agttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataataa                                                                                                                                          <  2:782563‑M2/7‑1 (MQ=255)       ttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATc                                                                                                                                        >  1:221134‑M2/135‑143 (MQ=255)        tagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCa                                                                                                                                       <  1:777043‑M2/10‑1 (MQ=255)           ttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgacatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAAtt                                                                                                                                    <  2:716756‑M2/13‑1 (MQ=255)           ttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAAtt                                                                                                                                    <  2:826977‑M2/13‑1 (MQ=255)           ttataaggatttccttaactgcttcgcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAAtt                                                                                                                                    <  1:285798‑M2/13‑1 (MQ=255)              taaggatttgcttaactgcttctgctcaccatcatgttgttttcgccacatcataatcctgggcttactgaagaataattgaaatgatattattaattccactgcatttggtagaggaaagggataaATAATAATCAATTGtt                                                                                                                                 >  1:395697‑M2/128‑143 (MQ=255)                       ccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctggacttgctgaagaataattgaaatgatattattaattccactgactttggtagaggaaagtgctaaATATTAATCATTTGTTAAATTATtg                                                                                                                        >  1:228832‑M2/119‑143 (MQ=255)                        cttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATtgt                                                                                                                       <  2:221134‑M2/26‑1 (MQ=255)                              tgcttctcctcaccgtcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCAttt                                                                                                                 <  1:818890‑M2/32‑1 (MQ=255)                                cttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCa                                                                                                               <  2:850909‑M2/34‑1 (MQ=255)                                 ttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAACTGTTAAATTATTGTGCATTTCac                                                                                                              >  1:576201‑M2/109‑143 (MQ=255)                                      ctcacgatcctgttattttcgccacctcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTg                                                                                                         <  2:567422‑M2/40‑1 (MQ=255)                                      ctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATACTCAATTGTTAAATTATTGTGCATTTCACTACTg                                                                                                         >  1:568196‑M2/104‑143 (MQ=255)                                      ctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTg                                                                                                         >  1:480973‑M2/104‑143 (MQ=255)                                                 gttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATc                                                                                              <  1:732068‑M2/51‑1 (MQ=255)                                                 gttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATc                                                                                              >  2:421353‑M2/93‑143 (MQ=255)                                                  ttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCa                                                                                             >  2:1142296‑M2/92‑143 (MQ=255)                                                  ttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTAACTACTGGAACTGTAATCa                                                                                             >  1:541345‑M2/92‑143 (MQ=255)                                                     ttttcgccacatcataatcctgagcttgctgaagaataattgaaatgatattattaattcaactgcctttggtataggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACT‑GAACTGTAATCAGaaa                                                                                         <  1:1017365‑M2/55‑1 (MQ=255)                                                      tttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaaa                                                                                         >  2:1006126‑M2/88‑143 (MQ=255)                                                       ttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaaaa                                                                                        >  1:328165‑M2/87‑143 (MQ=255)                                                       ttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaaaa                                                                                        <  1:388349‑M2/57‑1 (MQ=255)                                                       ttcgccaaatcataatcctggtcgtgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGTAACTGTAATCAGaaaa                                                                                        <  1:220570‑M2/57‑1 (MQ=255)                                                        tcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAg                                                                                       >  2:956895‑M2/86‑143 (MQ=255)                                                          gccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAt                                                                                     <  2:545489‑M2/60‑1 (MQ=255)                                                           gcacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATa                                                                                    <  1:389611‑M2/61‑1 (MQ=255)                                                           ccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATa                                                                                    <  1:1063178‑M2/61‑1 (MQ=255)                                                              catcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGAc                                                                                 <  2:217939‑M2/64‑1 (MQ=255)                                                                tcataatcctgggcctgctaaagaattatttaaatgatattattaattccactgcctttggtagacaaacttcctaaATCACAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACAt                                                                               <  1:82121‑M2/66‑1 (MQ=255)                                                                     cgcctgggcgtgctgaagaagaagtgagatgatatgatgaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGGTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTa                                                                          <  2:194850‑M2/71‑1 (MQ=255)                                                                        ctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGcc                                                                       <  1:662356‑M2/74‑1 (MQ=255)                                                                        ctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGcc                                                                       <  2:115241‑M2/74‑1 (MQ=255)                                                                          gggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCaa                                                                     >  2:1020237‑M2/68‑143 (MQ=255)                                                                           ggcctgcggaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAAt                                                                    <  1:640481‑M2/77‑1 (MQ=255)                                                                                  tgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTAtt                                                             >  1:647445‑M2/60‑143 (MQ=255)                                                                                   gaagaataattgaaatgatattattaattacactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATACACATGCTTAGCCAATCTCTAttt                                                            >  1:730148‑M2/59‑143 (MQ=255)                                                                                   gaagaataattgaaatgatattattaagtccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTAttt                                                            <  1:330208‑M2/85‑1 (MQ=255)                                                                                      gaataattgaaatgattttattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgat                                                         <  1:488909‑M2/88‑1 (MQ=255)                                                                                       aataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgatt                                                        >  2:342972‑M2/55‑143 (MQ=255)                                                                                            ctggaattatatgactaatccccctgcccgtggtagaggcaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGTTAGACATGCTTAGCCAATCTCTATTTGattgaatt                                                   <  2:646916‑M2/94‑1 (MQ=255)                                                                                               aaatgatcttattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGAttgaattgaa                                                <  1:137753‑M2/97‑1 (MQ=255)                                                                                                aattatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGaaa                                               <  1:169257‑M2/98‑1 (MQ=255)                                                                                                aatgattttattaattccactgcctttgttagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGaaa                                               <  2:323106‑M2/98‑1 (MQ=255)                                                                                                aatgatattattaattccactgcctttggtagaggaaagtgctaaGTAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATCTGATTGAATTGaaa                                               >  2:143620‑M2/46‑143 (MQ=255)                                                                                                  tgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATGTCTATTAGATTAAAGTGAACGa                                             >  1:1058580‑M2/44‑143 (MQ=255)                                                                                                  tgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGa                                             <  2:488987‑M2/100‑1 (MQ=255)                                                                                                   gatattattaattccactgcctttggtagagggagtgtcaaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGAAACTGTAAGCAGAAAAGATATACATGCTTATCCAATCTCTATTTGATTGAATTGAAAGAt                                            >  1:1091125‑M2/43‑143 (MQ=255)                                                                                                    atattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGCATTGAACGAtg                                           >  2:615213‑M2/42‑143 (MQ=255)                                                                                                     tattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgt                                          <  2:172458‑M2/103‑1 (MQ=255)                                                                                                       ttattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATGGAAAGAtgttt                                        >  2:650565‑M2/39‑143 (MQ=255)                                                                                                         attaattccactgcctttggtagaggacagtgctaaATAATAATCAATTGTTGAATTACCAACAAATTAACTACTTGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtttgt                                      >  1:888369‑M2/37‑143 (MQ=255)                                                                                                         attaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAAATATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGATTAGCCAATCTCTATTTAATTGAATTGAAAGAtgcttgt                                      >  1:1193126‑M2/37‑143 (MQ=255)                                                                                                          ttaattccactgcctttggtagaggaaagtgctaaATAATAATTAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAGGAtgtttgtt                                     >  1:686228‑M2/36‑143 (MQ=255)                                                                                                          ttaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCAGTTCACTACTGGAACTGTAATAAGAAAAGATAGAAATGCTTAGCCAATCCCTATTTGATTGAATTGAAAGAggtttgtt                                     >  1:70906‑M2/36‑143 (MQ=255)                                                                                                            aattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTaa                                   <  2:324695‑M2/110‑1 (MQ=255)                                                                                                             attccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAg                                  <  2:480973‑M2/111‑1 (MQ=255)                                                                                                             attccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAAATAAAAGATGATTGTTAAg                                  >  1:164254‑M2/33‑143 (MQ=255)                                                                                                               tccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTaagac                                >  1:567280‑M2/31‑141 (MQ=255)                                                                                                                 cactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCAt                              <  2:69736‑M2/115‑1 (MQ=255)                                                                                                                  actgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATg                             >  1:234984‑M2/28‑143 (MQ=255)                                                                                                                  actgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATg                             <  2:835628‑M2/116‑1 (MQ=255)                                                                                                                   ctgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATgg                            <  1:1020237‑M2/117‑1 (MQ=255)                                                                                                                     gcgtttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCGGAAAAGATAGACATGCTTGGCCGATCTCTATTTGATGGAATTGAAAGATGTTTGTTAAGGCATGGAt                          <  2:1189814‑M2/119‑1 (MQ=255)                                                                                                                     gcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGAt                          <  1:905768‑M2/119‑1 (MQ=255)                                                                                                                     accaaaggcagtggaaagcgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGAt                          <  1:184496‑M2/119‑1 (MQ=255)                                                                                                                      cctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAACAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATg                         >  2:577682‑M2/24‑143 (MQ=255)                                                                                                                      cctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAACGATGTTTGTTAAGGCATGGCTg                         >  1:1121654‑M2/24‑143 (MQ=255)                                                                                                                           ggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGc                    <  2:740660‑M2/125‑1 (MQ=255)                                                                                                                             tagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCta                  >  2:589625‑M2/17‑143 (MQ=255)                                                                                                                             tagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGACAATCTGTATTTGATTGAATTGAGAGATGTTTGTTAAGGCATGGATGAGAGCta                  >  1:1047843‑M2/17‑143 (MQ=255)                                                                                                                              agaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCtat                 <  2:805472‑M2/128‑1 (MQ=255)                                                                                                                               gaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCtata                >  1:848225‑M2/15‑143 (MQ=255)                                                                                                                                aggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAg               >  1:1112254‑M2/14‑143 (MQ=255)                                                                                                                                  gaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGAt             >  2:793927‑M2/12‑143 (MQ=255)                                                                                                                                   aaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGAtt            >  2:190868‑M2/11‑143 (MQ=255)                                                                                                                                       ggccaaAGAATAATCAATTGTTAAATTATTCTGCACTTCACTACTGGAACTTTAATCCGAGAAGTTGGACCTGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGa        <  1:442141‑M2/137‑1 (MQ=255)                                                                                                                                          taaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATAc     >  2:115368‑M2/4‑143 (MQ=255)                                                                                                                                           aaATAATAATGGATTGTTAAAGTATGGTGGATTTGACTAGTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACg    <  1:970216‑M2/141‑1 (MQ=255)                                                                                                                                             ataataatCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGt  <  2:1185632/143‑1 (MQ=255)                                                                                                                                             ataataagCAATTGTTAAATTATTGTGCATGTCACTACTGGAACTGTAAGCAGAAAAGATAGACTTGCTTAGCAAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGt  <  2:465544/143‑1 (MQ=255)                                                                                                                                             |                                                                                                                                               TAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGT  >  NC_000913/1300558‑1300840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑