Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 3,527,656 Δ5 bp coding (1190‑1194/2136 nt) yrfF → putative RcsCDB‑response attenuator, inner membrane protein

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3527656 3527660 5 27 [0] [0] 27 yrfF putative RcsCDB‑response attenuator, inner membrane protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 35276550 (0.000)27 (0.600) 23/284 1.1 100% coding (1189/2136 nt) yrfF putative RcsCDB‑response attenuator, inner membrane protein
?NC_000913 3527661 = 0 (0.000)coding (1195/2136 nt) yrfF putative RcsCDB‑response attenuator, inner membrane protein

GTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAAC  >  NC_000913/3527528‑3527793
                                                                                                                                     |                                                                                                                                    
atgctgttattctggatcccgctggatatgccgctgaaattcactctctcatggatgaaaggcgcgcagaccattgaagccaccagcgtaaaacaactggctgacgctggcgtgcggggaggcgatacattgcAGCGGTACgg                                                                                                                             >  1:540663‑M2/134‑143 (MQ=255)
    tgttattctggatcccgctggatatgccgctgaaattcactctctcatggatgtaaggcgcgcagaccattgaagccaccagcgtaaaacaaatggctgacgctagcgtgcggataggcgatacattgcAGCAGTACGGGAAt                                                                                                                         >  1:621881‑M2/130‑143 (MQ=255)
         ttttcgatcccgatggatctcatcctgaaattccctctctcatgcatgaaaggcgcgcagaccattgaacccaccagcgtaaaacaactgcctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTa                                                                                                                    <  1:39885‑M2/19‑1 (MQ=255)
          tctggatcccgctggatatgccgctgaaattcactctctcatggatgaaaggcgcgcagaccattgaagccaccagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTaa                                                                                                                   <  2:366379‑M2/20‑1 (MQ=255)
            cggatcccgctggatatgccgctgaaattcactctctcatggatgaaaggcgcgcagaccattgaagccaccagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTAata                                                                                                                 <  1:247293‑M2/22‑1 (MQ=255)
                     ctggatatgccgctgaaattcactctctcatgggtgaaaggcgcgcagaccattgaagccaccagcgtaaaacaactggctgacgctggcgtgcgggtaggcggtacattgcAGCGGTACGGGAATGTGGAATATtcgaagtg                                                                                                        >  2:31976‑M2/113‑140 (MQ=255)
                                      attcactctctcatggatgaaaggcgcgcagaccattgaagccaccagcgtaaaacaactggctgacgctggcgtgcgggtaggcgacacatcgcAGCGGTGCGGGAAGGTGTAATATTCCGACTTCCGGGACCTGGGGggcg                                                                                       >  2:396494‑M2/96‑143 (MQ=255)
                                                   tggatgaaaggcgcgcagaccattgaagccaccagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCAc                                                                          <  1:611258‑M2/61‑1 (MQ=255)
                                                    ggatgaaaggcgcgcagaccattgaagccaccagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCAcc                                                                         <  1:341578‑M2/62‑1 (MQ=255)
                                                        gaaaggcgcgtagatctttgaagttatcagagttaaacaactggctgacgtgggcgtgtgggtaggcgatacattgcAGCGGTAAGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCtttt                                                                     <  2:405608‑M2/66‑1 (MQ=255)
                                                        gaaaggcgcgcagaccattgaagccaccagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCGATTCACCtttt                                                                     >  1:15808‑M2/78‑143 (MQ=255)
                                                        gaaaggcgcgcagaccattgaagccaccagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCtttt                                                                     >  1:461636‑M2/78‑143 (MQ=255)
                                                             gcgcgcagaccattgaaggcaccagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTAcc                                                                <  2:508764‑M2/71‑1 (MQ=255)
                                                              cgcgcagaccattgaagccaccagcgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCg                                                               <  1:61305‑M2/72‑1 (MQ=255)
                                                                 gcagaccagtgaagccaccagcgtaaaacacctggcggacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGttt                                                            <  2:461636‑M2/75‑1 (MQ=255)
                                                                   agaccagtgaagcccccagcgtaagacaacgggcggacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGa                                                          <  2:668557‑M2/77‑1 (MQ=255)
                                                                              gcccccagcgtacaacaactggctgccgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGa                                               <  2:597503‑M2/88‑1 (MQ=255)
                                                                                tgccggcgtaaaccaactggctgacgcgggcgtgcgggtaggcgatacgttgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAACACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGatc                                             <  2:33250‑M2/90‑1 (MQ=255)
                                                                                cagcagcgtaacataacgggctgccgctggcgtgcgggtaggcgataccttgcAGTGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAACACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGatc                                             <  2:275380‑M2/90‑1 (MQ=255)
                                                                                      cgtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTttaccgtttgacttctctccggtcctccgg                                       >  1:414802‑M2/48‑130 (MQ=255)
                                                                                       gtaaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATCCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGa                                      >  2:267919‑M2/47‑143 (MQ=255)
                                                                                        taaaacaactggctgacgctggcgtgcgggtaggcgatacattgcAGCGGTACGGGAATGTGTAATGTTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCCGATAATCAGGaa                                     >  2:232177‑M2/46‑143 (MQ=255)
                                                                                                         gctggcgtgcgggtaggcgatacactgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGc                    <  1:268506‑M2/115‑1 (MQ=255)
                                                                                                                   gggtaggcgctacattgcAGCGGTACGGGTATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGGTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGa          <  2:606611‑M2/125‑1 (MQ=255)
                                                                                                                      taggcgatacattgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGACCATCTGGAACGACGCCCGCTCATTGCCATTCCCAGAATc       >  2:373381‑M2/16‑143 (MQ=255)
                                                                                                                           gatacattgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCTTTGCGTTTGCCAGaatctacac  >  1:322812‑M2/11‑139 (MQ=255)
                                                                                                                           gatacattgcAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAAc  >  1:414337‑M2/11‑143 (MQ=255)
                                                                                                                                     |                                                                                                                                    
GTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAAC  >  NC_000913/3527528‑3527793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.