Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 3,527,656 Δ5 bp coding (1190‑1194/2136 nt) yrfF → putative RcsCDB‑response attenuator, inner membrane protein

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3527656 3527660 5 27 [0] [0] 27 yrfF putative RcsCDB‑response attenuator, inner membrane protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 35276550 (0.000)27 (0.600) 23/284 1.1 100% coding (1189/2136 nt) yrfF putative RcsCDB‑response attenuator, inner membrane protein
?NC_000913 3527661 = 0 (0.000)coding (1195/2136 nt) yrfF putative RcsCDB‑response attenuator, inner membrane protein

ATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATC  >  NC_000913/3527523‑3527788
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aTGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGGAGGCGATACATTGCagcggtacgg                                                                                                                             >  1:540663‑M1/1‑133 (MQ=255)
    tGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGTAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAAATGGCTGACGCTAGCGTGCGGATAGGCGATACATTGCagcagtacgggaat                                                                                                                         >  1:621881‑M1/1‑129 (MQ=255)
         ttttcgatcccGATGGATCTCATCCTGAAATTCCCTCTCTCATGCATGAAAGGCGCGCAGACCATTGAACCCACCAGCGTAAAACAACTGCCTGACGCTGGCGTGCGGGTAGGCGATACATTGCagcggtacgggaatgtgta                                                                                                                    <  1:39885‑M1/138‑20 (MQ=255)
          tCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCagcggtacgggaatgtgtaa                                                                                                                   <  2:366379‑M1/143‑21 (MQ=255)
            cggaTCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCagcggtacgggaatgtgtaata                                                                                                                 <  1:247293‑M1/142‑23 (MQ=255)
                     cTGGATATGCCGCTGAAATTCACTCTCTCATGGGTGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGGTACATTGCagcggtacgggaatgtggaatattcgaagtg                                                                                                        >  2:31976‑M1/1‑112 (MQ=255)
                                      aTTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGACACATCGCagcggtgcgggaaggtgtaatattccgacttccgggacctggggggcg                                                                                       >  2:396494‑M1/1‑95 (MQ=255)
                                                   tGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCagcggtacgggaatgtgtaatattcgaacttccggaacctggagcgcgaaaaccaattcac                                                                          <  1:611258‑M1/143‑62 (MQ=255)
                                                    ggATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCagcggtacgggaatgtgtaatattcgaacttccggaacctggagcgcgaaaaccaattcacc                                                                         <  1:341578‑M1/143‑63 (MQ=255)
                                                        gAAAGGCGCGTAGATCTTTGAAGTTATCAGAGTTAAACAACTGGCTGACGTGGGCGTGTGGGTAGGCGATACATTGCagcggtaagggaatgtgtaatattcgaacttccggaacctggagcgcgaaaaccaattcacctttt                                                                     <  2:405608‑M1/143‑67 (MQ=255)
                                                        gAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCagcggtacgggaatgtgtaatattcgaacttccggaacctggagcgcgaaaaccgattcacctttt                                                                     >  1:15808‑M1/1‑77 (MQ=255)
                                                        gAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCagcggtacgggaatgtgtaatattcgaacttccggaacctggagcgcgaaaaccaattcacctttt                                                                     >  1:461636‑M1/1‑77 (MQ=255)
                                                             gcgcgcAGACCATTGAAGGCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCagcggtacgggaatgtgtaatattcgaacttccggaacctggagcgcgaaaaccaattcaccttttttacc                                                                <  2:508764‑M1/143‑72 (MQ=255)
                                                              cgcgcAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCagcggtacgggaatgtgtaatattcgaacttccggaacctggagcgcgaaaaccaattcaccttttttaccg                                                               <  1:61305‑M1/143‑73 (MQ=255)
                                                                 gcAGACCAGTGAAGCCACCAGCGTAAAACACCTGGCGGACGCTGGCGTGCGGGTAGGCGATACATTGCagcggtacgggaatgtgtaatattcgaacttccggaacctggagcgcgaaaaccaattcaccttttttaccgttt                                                            <  2:461636‑M1/143‑76 (MQ=255)
                                                                   aGACCAGTGAAGCCCCCAGCGTAAGACAACGGGCGGACGCTGGCGTGCGGGTAGGCGATACATTGCagcggtacgggaatgtgtaatattcgaacttccggaacctggagcgcgaaaaccaattcaccttttttaccgtttga                                                          <  2:668557‑M1/143‑78 (MQ=255)
                                                                              gCCCCCAGCGTACAACAACTGGCTGCCGCTGGCGTGCGGGTAGGCGATACATTGCagcggtacgggaatgtgtaatattcgaacttccggaacctggagcgcgaaaaccaattcaccttttttaccgtttgactgctcgcaga                                               <  2:597503‑M1/143‑89 (MQ=255)
                                                                                tgccggcgtaaACCAACTGGCTGACGCGGGCGTGCGGGTAGGCGATACGTTGCagcggtacgggaatgtgtaatattcgaacttccggaacctggagcgcgaacaccaattcaccttttttaccgtttgactgctcgcagatc                                             <  2:33250‑M1/138‑91 (MQ=255)
                                                                                cagcagcgTAACATAACGGGCTGCCGCTGGCGTGCGGGTAGGCGATACCTTGCagtggtacgggaatgtgtaatattcgaacttccggaacctggagcgcgaacaccaattcaccttttttaccgtttgactgctcgcagatc                                             <  2:275380‑M1/140‑91 (MQ=255)
                                                                                      cGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCagcggtacgggaatgtgtaatattcgaacttccggaacctggagcgcgaaaaccaattcaccttttttaccgtttgacttctctccggtcctccgg                                       >  1:414802‑M1/1‑47 (MQ=255)
                                                                                       gTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCagcggtacgggaatgtgtaatattcgaacttccggaacctggagcgcgaaaaccaatccaccttttttaccgtttgactgctcgcagatcatctgga                                      >  2:267919‑M1/1‑46 (MQ=255)
                                                                                        tAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCagcggtacgggaatgtgtaatgttcgaacttccggaacctggagcgcgaaaaccaattcaccttttttaccgtttgactgctcgccgataatcaggaa                                     >  2:232177‑M1/1‑45 (MQ=255)
                                                                                                         gCTGGCGTGCGGGTAGGCGATACACTGCagcggtacgggaatgtgtaatattcgaacttccggaacctggagcgcgaaaaccaattcaccttttttaccgtttgactgctcgcagatcatctggaacgacgcccgctcattgc                    <  1:268506‑M1/143‑116 (MQ=255)
                                                                                                                   gggTAGGCGCTACATTGCagcggtacgggtatgtgtaatattcgaacttccggaacctggagcgcgaaaaccaattcaccttttttaccggttgactgctcgcagatcatctggaacgacgcccgctcattgccattaccaga          <  2:606611‑M1/143‑126 (MQ=255)
                                                                                                                      tAGGCGATACATTGCagcggtacgggaatgtgtaatattcgaacttccggaacctggagcgcgaaaaccaattcaccttttttaccgtttgactgctcgcagaccatctggaacgacgcccgctcattgccattcccagaatc       >  2:373381‑M1/1‑15 (MQ=255)
                                                                                                                           gATACATTGCagcggtacgggaatgtgtaatattcgaacttccggaacctggagcgcgaaaaccaattcaccttttttaccgtttgactgctcgcagatcatctggaacgacgcccgctctttgcgtttgccagaatctacac  >  1:322812‑M1/1‑10 (MQ=255)
                                                                                                                           gATACATTGCagcggtacgggaatgtgtaatattcgaacttccggaacctggagcgcgaaaaccaattcaccttttttaccgtttgactgctcgcagatcatctggaacgacgcccgctcattgccattaccagaatctgaac  >  1:414337‑M1/1‑10 (MQ=255)
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ATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATC  >  NC_000913/3527523‑3527788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: