New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3263488	58 (1.450)	3 (0.080)	3/280	5.9	4.9%	coding (171/1209 nt)	tdcD	propionate kinase/acetate kinase C, anaerobic
	?	NC_000913	= 3263509	59 (1.500)					coding (150/1209 nt)	tdcD	propionate kinase/acetate kinase C, anaerobic
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3263365‑3263488 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggAGAGCCAGCACCGCTGGCTCACCACAGCTACGAAGGTGCATTGAAGGCAATTGCATTT  <  NC_000913/3263509‑3263452                                                                                                                                                                                        GGCGGACCCGGTAAAAATACTGCCGCCGTGAGCGATGCGGGGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGATGTGGTGAGCCAGCACCGCTGGCT                                         <  1:593632/143‑1                           CGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGAGAGCCAGCACCGCTGGCTCACCACAGCTACGGAGGTGCATTGAA               >  1:323543/1‑143                                        gttGCCACTTAAGTCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGAGAGCCAGCACCGCTGGCTCACCACAGCTACGAAGGTGCATTGAAGGCAATTGCATTT  <  2:323543/140‑1                                                                                                                                                                                        GGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3263365‑3263488 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggAGAGCCAGCACCGCTGGCTCACCACAGCTACGAAGGTGCATTGAAGGCAATTGCATTT  <  NC_000913/3263509‑3263452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.