New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3263488	58 (1.450)	3 (0.080)	3/280	5.9	4.9%	coding (171/1209 nt)	tdcD	propionate kinase/acetate kinase C, anaerobic
	?	NC_000913	= 3263509	59 (1.500)					coding (150/1209 nt)	tdcD	propionate kinase/acetate kinase C, anaerobic
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTT  >  NC_000913/3263349‑3263628                                                                                                                                            |                                                                                                                                             ggtgctCGGTAGTAATGGCGTACTCGGGAAAGATACTGCCGCCGTGGGCGATGCGGTGGCCAATTGAGGCCACACTGTCATTTAAGTTCCGTCTTCCCAGTGCAAATGCAATTGCCTTCAATGCACCTTCGTGGCTGTGGTGa                                                                                                                                           <  2:461072/138‑1 (MQ=255) cTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTAACTCTCATTCCCTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTgtcgtgg                                                                                                                                           >  1:320828/1‑142 (MQ=255)  atCATAGGGAATAATGGCGGACACGGTAAAAATACTGCCGCCGGGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAg                                                                                                                                          <  1:372685/142‑1 (MQ=255)       cGGTAATAATGGCGGACTCGGTAAAACTACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCACATGCAATTGCCTTCACTGCACCTTCGTAGCCGTGGTGAGCCCGc                                                                                                                                     >  2:104902/1‑143 (MQ=255)             taatGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCGCCTTCGTAGCTGTGGTGAGCCAGCGGTGCt                                                                                                                               >  2:387774/1‑143 (MQ=255)              aaggccGGACTCGCTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTg                                                                                                                              <  1:34734/138‑1 (MQ=255)                 ggCGGACCCGGTAAAAATACTGCCGCCGTGAGCGATGCGGGGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGATGTGGTGAGCCAGcaccgctggct                                                                                                                           <  1:593632/143‑11 (MQ=255)                  gCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATCCCCCCTCGTCCCTCTGGTGCGCCCGCGGGCCTGGccc                                                                                                                          >  1:216752/1‑141 (MQ=255)                       cTCGGTAAAAATAAGGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTTCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTcccc                                                                                                                     <  1:225365/143‑1 (MQ=255)                           gTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCACTGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCCCTcccccgtc                                                                                                                 >  1:335853/1‑140 (MQ=255)                           gTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCAtt                                                                                                                 >  1:460049/1‑143 (MQ=255)                                aaTACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCCTTTACgg                                                                                                            >  1:463483/1‑143 (MQ=255)                                 atcctgctgCCTTGATCGATGCCGTGGTCAATTAACGACACATTGTCATTTCAACTCCGTTTTTCAAGTTACAATGCACATGCCTCCACTACACCCACGCAACTGTAGACCGTCAGCGGTGCTGGCTCTCCCCCATTTACGGa                                                                                                           <  2:649139/140‑1 (MQ=255)                                     tGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGGGCTGGCTCTCCCCCATTTACGGATAAg                                                                                                       >  1:185656/1‑143 (MQ=255)                                       ccgccgTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGaa                                                                                                     >  1:240754/1‑143 (MQ=255)                                         gccgTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATg                                                                                                   <  1:168763/143‑1 (MQ=255)                                             tGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATCTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCAtt                                                                                               <  1:29052/143‑1 (MQ=255)                                               aGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCACATGCAGATGACTTAAGTGCACCTTCGTAGCTGTGGTGCGCAAGCGGTGATGGGTCTCCCCCATTTACGGATAAGAATGCAtttt                                                                                             <  2:279874/143‑1 (MQ=255)                                                   aTGCGTTTGCCAATTACGGCCACACTGTCATTTAAATGCCGTGTTTCCAGTTCAAATGCGATTGCCTTCAGTGCACCTTCGTAACTGTGCTGAGCCAGCGGTGCTCGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGa                                                                                         <  2:421467/143‑1 (MQ=255)                                                        gTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTaa                                                                                    >  1:686273/1‑143 (MQ=255)                                                          ggCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATa                                                                                  <  1:633740/143‑1 (MQ=255)                                                           gCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCTGAGTTAATAc                                                                                 >  1:193431/1‑143 (MQ=255)                                                           gCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATAc                                                                                 >  1:466260/1‑143 (MQ=255)                                                           gCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATCTACGGCTCAGCATGCATTTTCCGAGTCAATAc                                                                                 >  2:260065/1‑143 (MQ=255)                                                            ccAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTCAATAcc                                                                                >  1:410712/1‑143 (MQ=255)                                                             cAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCCCCCCCATTTACGGCTAAGAATGCGTTTTCCGAGTTaaggccg                                                                               >  1:186723/1‑138 (MQ=255)                                                                ttAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCg                                                                            <  2:584547/143‑1 (MQ=255)                                                                ttAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATACAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGGTAATACCGttc                                                                            >  2:183466/1‑141 (MQ=255)                                                                 tAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCACTTGCCCTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCgg                                                                           <  2:335118/143‑1 (MQ=255)                                                                 caaGGCCACACTGTCATTTAAATTCCGTTGTTCCAGTGCAAATGCAATTGCCTTCAATGCACCTTCGTCGCTGTGGTCATCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCgg                                                                           <  2:458747/142‑1 (MQ=255)                                                                  aaGGCCCCACTGTCGTCTAAATTCCGTTTTTTCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTTGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGc                                                                          <  2:106104/143‑1 (MQ=255)                                                                   aGGCCACACTGTCATTTAAATTCCCTTTTTCCAATTGAAATGCAATTGCCTTCAATGCACCTTCGTACATATAGTATACGATTTGTTTTGGCTCGCCCCCAGTTACGGGGGAGAATGCAGTTTCCGAGTTAAGACCGTCGGCa                                                                         >  2:208170/1‑143 (MQ=255)                                                                     gCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAACGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCGTTTTCCGCGTTCATACCGTCGGCCAt                                                                       >  1:337578/1‑143 (MQ=255)                                                                     gCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCAGTGGTGAGCCAGCGATACCGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGCTAATACCGTCGGCaaa                                                                       >  2:242794/1‑142 (MQ=255)                                                                       cacaCTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTACTACCGTCGACAATAc                                                                     >  2:668669/1‑143 (MQ=255)                                                                          acTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTTTGGTGAGCCAGCGGTGCTGGCTCTCCCCCCTTTACGGCTCCTAATGCATTTTCCCAGTTAATACCGTCGGCCCTACCTg                                                                  >  1:633016/1‑143 (MQ=255)                                                                                   tAAAGTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATa                                                         <  1:617573/143‑1 (MQ=255)                                                                                        tCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGGTAATACCGTCGGCAATACCTGCCAGTAATACTTca                                                    >  2:672870/1‑143 (MQ=255)                                                                                          cgttaatacatggtaaaTGCAATTGCCTTCAATGCACCTTCGTAGCTGTAGTGAGCCAGCGGTGCTGGCTCCCCCCCATTTACGGATAAGAATGCATTTTACCAGTTAATACCGTCGGCAATACCTGACATTAATACTTcaca                                                  >  2:119789/15‑143 (MQ=255)                                                                                          cGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGCATGCATTGTCCGAGTTCATACCGACCGCGCTACCTGACGTTATTACTTcatc                                                  >  2:128966/1‑141 (MQ=255)                                                                                           gTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATCCCTGACATTAATACTTGACAg                                                 >  1:575599/1‑143 (MQ=255)                                                                                                tCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCt                                            <  2:131786/143‑1 (MQ=255)                                                                                                 ccAGTTCAAATGCAATTGTCTTCAATGCACCTTCGCAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTg                                           <  1:621590/143‑1 (MQ=255)                                                                                                  cAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTgg                                          >  1:80893/1‑143 (MQ=255)                                                                                                  cAGTCCAAATGCACTTGCCTTCGTTGTACCTTCGTAGCTGTGGTGAGGCAGCGGTGCTGGCTCTCCTCCATTTACGTATACGAATTCGTTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTgg                                          <  2:208531/143‑1 (MQ=255)                                                                                                     ttCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCAt                                       >  1:678000/1‑143 (MQ=255)                                                                                                     ttCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATGAATACTGCACAGTCGCTGGCAt                                       >  1:644673/1‑143 (MQ=255)                                                                                                        aaaTGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACGTTGATACTTCACAGTCGCTGGTGTCGa                                    >  1:13074/1‑143 (MQ=255)                                                                                                                 tGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGaaa                           >  2:18026/1‑143 (MQ=255)                                                                                                                   ccTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATACAATTACCAATTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAAc                         <  1:97687/143‑1 (MQ=255)                                                                                                                      tgaacccACCTTCGTAGATGTGGTGAGCCCGCGGTTCTGGCCCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTa                      <  1:401122/137‑1 (MQ=255)                                                                                                                       ccttGCACCTGCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTaa                     <  2:380384/140‑1 (MQ=255)                                                                                                                       cAACGCACCTGCGTAGGTGTGCTGAGCCAGCGGTGCTGGTTCGCCCCCACTTACGAATAAGAAAGCATATTCCGAGTTAAGACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTaa                     <  2:81124/143‑1 (MQ=255)                                                                                                                         aTGCACCTTCGTGGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATc                   <  1:128460/143‑1 (MQ=255)                                                                                                                           gCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGATAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGACAACTTAATCGa                 >  1:408686/1‑143 (MQ=255)                                                                                                                              cctgctgttgcgtggtgAGCCAGCGGTGCTGGCGCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGa              <  2:479302/132‑1 (MQ=255)                                                                                                                                ttCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCGATACCTGACATTACTACTTCACAGTCGCTGGCATCGAGCACGGAAAACTCAATCGAAGCCg            >  1:31076/1‑143 (MQ=255)                                                                                                                                       cTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGACAACTTAATCGAGGACGAACcaca     >  1:200845/1‑143 (MQ=255)                                                                                                                                          tggtgAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGtt  >  1:78630/1‑143 (MQ=255)                                                                                                                                            |                                                                                                                                             CTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTT  >  NC_000913/3263349‑3263628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.