New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 326348858 (1.450)3 (0.080) 3/280 5.9 4.9% coding (171/1209 nt) tdcD propionate kinase/acetate kinase C, anaerobic
?NC_000913 = 3263509 59 (1.500)coding (150/1209 nt) tdcD propionate kinase/acetate kinase C, anaerobic
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAA  >  NC_000913/3263371‑3263649
                                                                                                                                          |                                                                                                                                            
cTCGGTAAAAATAAGGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTTCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTcccc                                                                                                                                          <  1:225365/143‑1 (MQ=255)
    gTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCACTGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCCCTcccccgtc                                                                                                                                      >  1:335853/1‑140 (MQ=255)
    gTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCAtt                                                                                                                                      >  1:460049/1‑143 (MQ=255)
         aaTACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCCTTTACgg                                                                                                                                 >  1:463483/1‑143 (MQ=255)
          atcctgctgCCTTGATCGATGCCGTGGTCAATTAACGACACATTGTCATTTCAACTCCGTTTTTCAAGTTACAATGCACATGCCTCCACTACACCCACGCAACTGTAGACCGTCAGCGGTGCTGGCTCTCCCCCATTTACGGa                                                                                                                                <  2:649139/140‑1 (MQ=255)
              tGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGGGCTGGCTCTCCCCCATTTACGGATAAg                                                                                                                            >  1:185656/1‑143 (MQ=255)
                ccgccgTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGaa                                                                                                                          >  1:240754/1‑143 (MQ=255)
                  gccgTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATg                                                                                                                        <  1:168763/143‑1 (MQ=255)
                      tGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATCTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCAtt                                                                                                                    <  1:29052/143‑1 (MQ=255)
                        aGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCACATGCAGATGACTTAAGTGCACCTTCGTAGCTGTGGTGCGCAAGCGGTGATGGGTCTCCCCCATTTACGGATAAGAATGCAtttt                                                                                                                  <  2:279874/143‑1 (MQ=255)
                            aTGCGTTTGCCAATTACGGCCACACTGTCATTTAAATGCCGTGTTTCCAGTTCAAATGCGATTGCCTTCAGTGCACCTTCGTAACTGTGCTGAGCCAGCGGTGCTCGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGa                                                                                                              <  2:421467/143‑1 (MQ=255)
                                 gTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTaa                                                                                                         >  1:686273/1‑143 (MQ=255)
                                   ggCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATa                                                                                                       <  1:633740/143‑1 (MQ=255)
                                    gCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCTGAGTTAATAc                                                                                                      >  1:193431/1‑143 (MQ=255)
                                    gCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATAc                                                                                                      >  1:466260/1‑143 (MQ=255)
                                    gCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATCTACGGCTCAGCATGCATTTTCCGAGTCAATAc                                                                                                      >  2:260065/1‑143 (MQ=255)
                                     ccAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTCAATAcc                                                                                                     >  1:410712/1‑143 (MQ=255)
                                      cAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCCCCCCCATTTACGGCTAAGAATGCGTTTTCCGAGTTaaggccg                                                                                                    >  1:186723/1‑138 (MQ=255)
                                         ttAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCg                                                                                                 <  2:584547/143‑1 (MQ=255)
                                         ttAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATACAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGGTAATACCGttc                                                                                                 >  2:183466/1‑141 (MQ=255)
                                          tAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCACTTGCCCTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCgg                                                                                                <  2:335118/143‑1 (MQ=255)
                                          caaGGCCACACTGTCATTTAAATTCCGTTGTTCCAGTGCAAATGCAATTGCCTTCAATGCACCTTCGTCGCTGTGGTCATCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCgg                                                                                                <  2:458747/142‑1 (MQ=255)
                                           aaGGCCCCACTGTCGTCTAAATTCCGTTTTTTCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTTGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGc                                                                                               <  2:106104/143‑1 (MQ=255)
                                            aGGCCACACTGTCATTTAAATTCCCTTTTTCCAATTGAAATGCAATTGCCTTCAATGCACCTTCGTACATATAGTATACGATTTGTTTTGGCTCGCCCCCAGTTACGGGGGAGAATGCAGTTTCCGAGTTAAGACCGTCGGCa                                                                                              >  2:208170/1‑143 (MQ=255)
                                              gCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAACGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCGTTTTCCGCGTTCATACCGTCGGCCAt                                                                                            >  1:337578/1‑143 (MQ=255)
                                              gCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCAGTGGTGAGCCAGCGATACCGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGCTAATACCGTCGGCaaa                                                                                            >  2:242794/1‑142 (MQ=255)
                                                cacaCTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTACTACCGTCGACAATAc                                                                                          >  2:668669/1‑143 (MQ=255)
                                                   acTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTTTGGTGAGCCAGCGGTGCTGGCTCTCCCCCCTTTACGGCTCCTAATGCATTTTCCCAGTTAATACCGTCGGCCCTACCTg                                                                                       >  1:633016/1‑143 (MQ=255)
                                                            tAAAGTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATa                                                                              <  1:617573/143‑1 (MQ=255)
                                                                 tCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGGTAATACCGTCGGCAATACCTGCCAGTAATACTTca                                                                         >  2:672870/1‑143 (MQ=255)
                                                                   cgttaatacatggtaaaTGCAATTGCCTTCAATGCACCTTCGTAGCTGTAGTGAGCCAGCGGTGCTGGCTCCCCCCCATTTACGGATAAGAATGCATTTTACCAGTTAATACCGTCGGCAATACCTGACATTAATACTTcaca                                                                       >  2:119789/15‑143 (MQ=255)
                                                                   cGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGCATGCATTGTCCGAGTTCATACCGACCGCGCTACCTGACGTTATTACTTcatc                                                                       >  2:128966/1‑141 (MQ=255)
                                                                    gTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATCCCTGACATTAATACTTGACAg                                                                      >  1:575599/1‑143 (MQ=255)
                                                                         tCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCt                                                                 <  2:131786/143‑1 (MQ=255)
                                                                          ccAGTTCAAATGCAATTGTCTTCAATGCACCTTCGCAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTg                                                                <  1:621590/143‑1 (MQ=255)
                                                                           cAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTgg                                                               >  1:80893/1‑143 (MQ=255)
                                                                           cAGTCCAAATGCACTTGCCTTCGTTGTACCTTCGTAGCTGTGGTGAGGCAGCGGTGCTGGCTCTCCTCCATTTACGTATACGAATTCGTTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTgg                                                               <  2:208531/143‑1 (MQ=255)
                                                                              ttCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCAt                                                            >  1:678000/1‑143 (MQ=255)
                                                                              ttCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATGAATACTGCACAGTCGCTGGCAt                                                            >  1:644673/1‑143 (MQ=255)
                                                                                 aaaTGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACGTTGATACTTCACAGTCGCTGGTGTCGa                                                         >  1:13074/1‑143 (MQ=255)
                                                                                          tGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGaaa                                                >  2:18026/1‑143 (MQ=255)
                                                                                            ccTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATACAATTACCAATTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAAc                                              <  1:97687/143‑1 (MQ=255)
                                                                                               tgaacccACCTTCGTAGATGTGGTGAGCCCGCGGTTCTGGCCCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTa                                           <  1:401122/137‑1 (MQ=255)
                                                                                                ccttGCACCTGCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTaa                                          <  2:380384/140‑1 (MQ=255)
                                                                                                cAACGCACCTGCGTAGGTGTGCTGAGCCAGCGGTGCTGGTTCGCCCCCACTTACGAATAAGAAAGCATATTCCGAGTTAAGACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTaa                                          <  2:81124/143‑1 (MQ=255)
                                                                                                  aTGCACCTTCGTGGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATc                                        <  1:128460/143‑1 (MQ=255)
                                                                                                    gCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGATAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGACAACTTAATCGa                                      >  1:408686/1‑143 (MQ=255)
                                                                                                       cctgctgttgcgtggtgAGCCAGCGGTGCTGGCGCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGa                                   <  2:479302/132‑1 (MQ=255)
                                                                                                         ttCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCGATACCTGACATTACTACTTCACAGTCGCTGGCATCGAGCACGGAAAACTCAATCGAAGCCg                                 >  1:31076/1‑143 (MQ=255)
                                                                                                                cTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGACAACTTAATCGAGGACGAACcaca                          >  1:200845/1‑143 (MQ=255)
                                                                                                                   tggtgAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGtt                       >  1:78630/1‑143 (MQ=255)
                                                                                                                    ggagagccagcaccgCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTta                      >  1:37711/15‑143 (MQ=255)
                                                                                                                    ggagAGCCAGCACCGCTGGCACTCCCCCATTTACGGATAAGAATGCATTTTCCGAGGTAATACCGTCGGCCATACCTGACATTAATACTTCACAGTCGCTGCCATCGAGCACGGAAAAGTTCATCGAAGACGCACCACAATta                      >  2:367109/4‑143 (MQ=255)
                                                                                                                       gAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTtaata                   <  2:694016/143‑1 (MQ=255)
                                                                                                                          ccAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAAGGCATTTTCCGAGTGAATACCGTCGGCAGTACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAGAACTTAATCGAAGACGAACCACAGTTAATAAcc                >  1:214944/1‑143 (MQ=255)
                                                                                                                             gCGGTGCCGGCTCTCCCCCATTTATGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCaaa             <  1:387774/143‑1 (MQ=255)
                                                                                                                              cGGTGCTGGCTCTCCCCCATGTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTGCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCaaaa            <  2:319990/143‑1 (MQ=255)
                                                                                                                                        tctcCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGaaa  >  1:577564/1‑143 (MQ=255)
                                                                                                                                        tctcACCCATTTACGGATAAGAATCCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGaaa  <  1:696552/143‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                            
CTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAA  >  NC_000913/3263371‑3263649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.