Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 257,908 Δ776 bp [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 257908–258674 258683 10–776 51 [0] [0] 52 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2579070 (0.000)48 (1.020) 42/266 0.1 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers

GTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGT  >  NC_000913/258542‑258826
                                                                                                                                              |                                                                                                                                               
tgttaaaaattttctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaa                                                                                                                                                 <  2:344506‑M2/1‑1 (MQ=255)
            gctaaattttgccaatttggtccaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATaa                                                                                                                                     <  1:213715‑M2/13‑1 (MQ=255)
             ctaaattttgccaattgggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAAt                                                                                                                                    <  2:755871‑M2/14‑1 (MQ=255)
              taaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgacgagcagattgatcaaaaaatttaccgcacgacgcccgtatagtcgtgaagctaAGTGCAAAGATAATc                                                                                                                                   >  1:203138‑M2/129‑143 (MQ=255)
               aaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagagtgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCg                                                                                                                                  >  1:82980‑M2/128‑143 (MQ=255)
                aattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGa                                                                                                                                 >  2:353644‑M2/127‑143 (MQ=255)
                     cgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccaagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgcgaaggtaAGTGCAAAGATAATCGATTCtt                                                                                                                           <  2:67460‑M2/22‑1 (MQ=255)
                          atttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCg                                                                                                                      <  2:716588‑M2/27‑1 (MQ=255)
                                     cagttgcatcacaacaggagatagcaatgacgttaccgagtggacagcgggggggcacacatatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCt                                                                                                           <  2:403891‑M2/38‑1 (MQ=255)
                                         tgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTAt                                                                                                       <  2:839759‑M2/42‑1 (MQ=255)
                                           catcacaacgggagatagcaatgacgttaccgagtggacagccgaagagcagattgatcaaaaaatttaccgcactagtcccgtatattcgtgaaggtaATTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGc                                                                                                     <  1:194904‑M2/44‑1 (MQ=255)
                                            atcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCg                                                                                                    >  2:261079‑M2/99‑143 (MQ=255)
                                             ccacagcaggagatagcaatgacgtgaccgagtggacacccgaagagcagattgatcaaaaaatttactgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGt                                                                                                   <  1:648192‑M2/46‑1 (MQ=255)
                                                    aggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAAGCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTg                                                                                            <  2:557817‑M2/53‑1 (MQ=255)
                                                    agagaatagaaatgacgttacctagtggacacgcgaagagcagattgatcaaaaaattttccgcactaggcccgtatattcgtgaaggtaAGTGCAACGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTg                                                                                            <  1:81533‑M2/53‑1 (MQ=255)
                                                         atagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatcttcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGCAAcc                                                                                       >  2:242412‑M2/86‑143 (MQ=255)
                                                         atagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAAcc                                                                                       >  2:743623‑M2/86‑143 (MQ=255)
                                                          tagcaatgacgttaccgagtggacacccgaatagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCg                                                                                      <  1:867590‑M2/59‑1 (MQ=255)
                                                           tgcgaggacgggaccgagcggacgcccgaagagcagattgagcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCgg                                                                                     <  2:572357‑M2/60‑1 (MQ=255)
                                                           agcaatgacgttaccgaggggacacccgaagagccgagtgatcaaaaaatttcccgcactaggcccgtatattcgtgaaggtaAGTGCAACGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCgg                                                                                     <  2:6406‑M2/60‑1 (MQ=255)
                                                           agcaaggacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCgg                                                                                     <  2:807730‑M2/60‑1 (MQ=255)
                                                              aatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCAc                                                                                  <  2:843529‑M2/63‑1 (MQ=255)
                                                                   ggttaccgagtggccacccgaagagcagactgatcaaaaaattgaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGaa                                                                             <  2:188855‑M2/68‑1 (MQ=255)
                                                                    gttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAGCTCGGGACCGGCAg                                                                            >  2:183785‑M2/75‑143 (MQ=255)
                                                                       accgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGCCTGTCTGCGTCGACGTGAAACCGTCCCCGGAcgtcc                                                                         >  2:728636‑M2/72‑141 (MQ=255)
                                                                        ccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCg                                                                        <  2:578477‑M2/73‑1 (MQ=255)
                                                                         cgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGt                                                                       <  1:107769‑M2/74‑1 (MQ=255)
                                                                               gacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTc                                                                 <  2:813118‑M2/80‑1 (MQ=255)
                                                                                   cccgaagaccagctttatcaaaacatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTTCGATTGTCTGGCTGTATTCGTCAACGTGAAACCGGCACCTGAAGTGCGTGAATTCTgg                                                              <  1:210639‑M2/84‑1 (MQ=255)
                                                                                          agcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggtg                                                      >  2:524799‑M2/53‑143 (MQ=255)
                                                                                               attgatcaaacaatttacctcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggatgg                                                 <  2:209621‑M2/96‑1 (MQ=255)
                                                                                                   atcaaaaaattttccgcactaggcccgtatattcgtaaaagtaAGTGGATGGGTGGTCGATTCTTTTTCGATGGTCGGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCt                                             <  2:239880‑M2/100‑1 (MQ=255)
                                                                                                        gaaagttaacgaacgaggcacgtatagtcaggaaggtaAGTGCAAAGGTAATCGATTCTTGTTCGATTGTCTGGCTGTATGTGCCAACGTGAAAGCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAg                                        <  1:211169‑M2/105‑1 (MQ=255)
                                                                                                            tttcccgccctaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAGCCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCa                                    <  1:580197‑M2/109‑1 (MQ=255)
                                                                                                                acgaaatagccccggatgttcgtgaaggtaAGTGGAAAGACAATCGATGCTGGTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGGACCGGAAGAGCGTTAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGaa                                <  1:392385‑M2/113‑1 (MQ=255)
                                                                                                                 cgcactaggcccgtatattcgtgaacgtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTCACTCCTGCGGCTGGTGGATGAAGCCGCACCCgcaggccc                               >  1:611497‑M2/30‑140 (MQ=255)
                                                                                                                     ataggcccctatattcgtgaaggtaAGTGCAAAGCTACACGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCg                           <  1:710567‑M2/118‑1 (MQ=255)
                                                                                                                      taggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGGTGGAGCTGGAAGCGCAGGAATCCCGt                          >  2:469416‑M2/25‑143 (MQ=255)
                                                                                                                       aggcccgtatattcgtgaaggtaAGTTCCAAGATAATAGAAGTGTGGCGGGTGGTCCGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGtt                         <  2:732239‑M2/120‑1 (MQ=255)
                                                                                                                        gtccggtgtattcgggaaggtaAGTGCAAAGATCATCGATTCTTGTTCGATTGTGTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGttt                        <  2:390110‑M2/121‑1 (MQ=255)
                                                                                                                           ccgtgtattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTAc                     <  2:747004‑M2/124‑1 (MQ=255)
                                                                                                                           ccgtatgtttgtgaaggtaAGTGCAAAGATAATCGATGCTTTTTCGATGGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTAc                     <  1:560454‑M2/124‑1 (MQ=255)
                                                                                                                           ccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCAAACGAACACGGTGTGGTTTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTAc                     >  2:639613‑M2/20‑143 (MQ=255)
                                                                                                                             gtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGACACCGGCACCGGACGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCt                   >  2:78896‑M2/18‑143 (MQ=255)
                                                                                                                                tattcgtgtaggtaAGTGCAAGGATAATCGATTCTTTTTCGATGGGCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACa                <  1:408233‑M2/129‑1 (MQ=255)
                                                                                                                                tattcgggagggtaAGTGCACAGACAATCGATTCTTTTTCGATTGGCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGTATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACa                <  1:75601‑M2/129‑1 (MQ=255)
                                                                                                                                 attcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGACTGGTGGCTTGGGCTGGAAGCGAAGGAATCTCGTTTTCCTTACAg               >  2:542228‑M2/14‑143 (MQ=255)
                                                                                                                                   tcgtgaagggaAGCGCAAAGAGAATCGATTCTTTTTCGAGTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATGCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGtt             <  2:76657‑M2/132‑1 (MQ=255)
                                                                                                                                     gcgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTAc           <  2:64031‑M2/134‑1 (MQ=255)
                                                                                                                                       gcacgtaAGTGCAAAGCTAATCGATTCTTTTTCGATTGTCGGGCTGTATGCGTCAACGTGGAACCGGCACCGGACGTGCGTGAATTCTGGGGCTGGTGGACGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCa         <  1:165269‑M2/136‑1 (MQ=255)
                                                                                                                                          gggaAGTGCAAAGATAATCGATGCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGtt      <  2:149392‑M2/139‑1 (MQ=255)
                                                                                                                                              aGTGCAAAGATAATCGATTCTGTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGt  <  2:740281/143‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                               
GTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGT  >  NC_000913/258542‑258826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: