breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 759,544 A→G 9.7% D280G (GAC→GGC)  sucA → 2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding
RA 1,068,294 C→G 8.7% intergenic (+40/+217) ymdF → / ← rutG KGG family protein/pyrimidine permease
RA 1,413,542 A→T 100% Y67N (TAT→AAT)  ydaC ← DUF1187 family protein, Rac prophage; putative double‑strand break reduction protein
RA 1,535,527 Δ1 bp 17.6% coding (332/894 nt) yddE ← PhzC‑PhzF family protein
RA 1,793,897 Δ1 bp 100% coding (213/552 nt) btuE ← glutathione peroxidase
RA 1,825,023 T→A 11.0% intergenic (‑86/+117) ves ← / ← spy cold‑ and stress‑inducible protein/periplasmic ATP‑independent protein refolding chaperone, stress‑induced
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
RA 2,045,098 Δ1 bp 14.3% coding (161/7077 nt) yeeJ → putative adhesin
RA 2,045,099 Δ1 bp 14.3% coding (162/7077 nt) yeeJ → putative adhesin
RA 2,050,073 C→T 8.0% S1712S (AGC→AGT yeeJ → putative adhesin
RA 2,093,040 G→A 100% A215T (GCT→ACT)  hisC → histidinol‑phosphate aminotransferase
RA 2,876,477 C→A 10.9% intergenic (‑150/‑102) cysD ← / → iap sulfate adenylyltransferase, subunit 2/aminopeptidase in alkaline phosphatase isozyme conversion
RA 3,223,086 G→T 6.3% K152N (AAG→AAT ebgA → evolved beta‑D‑galactosidase, alpha subunit
JC 3,972,029 +CCTGGC 26.8% coding (771/1263 nt) wecC → UDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase
RA 3,975,297 Δ1 bp 69.4% coding (153/1131 nt) wecE → TDP‑4‑oxo‑6‑deoxy‑D‑glucose transaminase
RA 3,975,298 Δ1 bp 70.6% coding (154/1131 nt) wecE → TDP‑4‑oxo‑6‑deoxy‑D‑glucose transaminase
RA 4,117,004 T→A 100% D73V (GAT→GTT)  glpK ← glycerol kinase
MC JC 4,188,484 Δ27 bp 100% coding (3136‑3162/4224 nt) rpoC → RNA polymerase, beta prime subunit
RA 4,508,694 A→T 14.3% pseudogene (250/267 nt) insM ← pseudogene, IS600 transposase;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 366210 366403 194 11 [10] [10] 11 [lacZ] [lacZ]
* * ÷ NC_000913 3423753–3424232 3424521–3424236 5–769 11 [10] [10] 11 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =16 (0.330)18 (0.420) 17/244 NT 51.3% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 20 (0.470)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780516 (0.330)15 (0.350) 15/244 NT 46.8% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 20 (0.470)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)52 (1.110) 43/268 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein