breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp [crl] [crl]
RA 1,685,527 A→G G354G (GGT→GGC fumC ← fumarate hydratase (fumarase C),aerobic Class II
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 2,145,313 C→A S683Y (TCC→TAC)  yegE → putative diguanylate cyclase
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,318,095 C→G L594F (TTG→TTC rcsC ← hybrid sensory kinase in two‑component regulatory system with RcsB and YojN
RA 3,486,517 G→A G133D (GGC→GAC)  crp → cAMP‑activated global transcription factor, mediator of catabolite repression
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 4,117,005 T→A D73V (GAT→GTT)  glpK ← glycerol kinase
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 2033596 2034094 499 39 [37] [37] 39 rseX–[yedS] rseX,[yedS]
* * ÷ NC_000913 2469142 2469850 709 39 [38] [38] 39 gtrS serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)50 (0.480) 41/508 1.5 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3721045 =115 (1.090)288 (2.740) 133/514 0.0 71.5% noncoding (413/1443 nt) IS150 repeat region
?NC_000913 = 3839774 115 (1.090)coding (220/819 nt) nlpA cytoplasmic membrane lipoprotein‑28