New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 4530145180 (1.300)4 (0.030) 4/214 NT 2.2% coding (99/1314 nt) sgcC putative PTS system EIIC permease component
?NC_000913 = 4530173 183 (1.400)coding (71/1314 nt) sgcC putative PTS system EIIC permease component
Rejected: Frequency below/above cutoff threshold.

GCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4529992‑4530145
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gccttaTCTTCCGCATCCCCTGGCTGCAGGCGATTAAGGCAGGGGTAACGGTTGGGATTGGTTTTGTCGGTATGGGACTGGTGATCGTCATGGCGATCGACAGCCTCAGTCCCCCTATCAAAGTGATGATTGAGCGTTTCGGCCTAGCCCTGC  <  NC_000913/4530173‑4530027
                                                                                                                                                                                                                                                                                                             
GCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTG                                                                                                                                                          >  4:115614/1‑149
 CCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGC                                                                                                                                                         <  3:115614/149‑1
     AGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTA                                                                                                                                                     >  2:770304/1‑149
                      ACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTATCTTCCGCATCCCCTGG                                                                                                                                    >  2:1238153/1‑149
                                           AGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTATCTTCCGCATCCCCTGGCTGCAGGCGATTAAGGCAGGG                                                                                                               <  1:1238153/149‑1
                                                                                        TCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTATCTTCCGCATCCCCTGGCTGCAGGCGATTAAGG                                                                                                                    <  1:379699/99‑1
                                                                                        TCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTATCTTCCGCATCCCCTGGCTGCAGGCGATTAAGG                                                                                                                    >  2:379699/1‑99
                                                                                                                                                    GCCTTATCTTCCGCATCCCCTGGCTGCAGGCGATTAAGGCAGGGGTAACGGTTGGGATTGGTTTTGTCGGTATGGGACTGGTGATCGTCATGGCGATCGACAGCCTCAGTCCCCCTATCAAAGTGATGATTGAGCGTTTCGGCCTAGCC      <  2:1119589/149‑1
                                                                                                                                                     CCTTATCTTCCGCATCCCCTGGCTGCAGGCGATTAAGGCAGGGGTAACGGTTGGGATTGGTTTTGTCGGTATGGGACTGGTGATCGTCATGGCGATCGACAGCCTCAGTCCCCCTATCAAAGTGATGATTGAGCGTTTCGGCCTAGCCC     >  3:1071294/1‑149
                                                                                                                                                     CCTTATCTTCCGCATCCCCTGGCTGCAGGCGATTAAGGCAGGGGTAACGGTTGGGATTGGTTTTGTCGGTATGGGACTGGTGATCGTCATGGCGATCGACAGCCTCAGTCCCCCT                                       <  1:36721/115‑1
                                                                                                                                                     CCTTATCTTCCGCATCCCCTGGCTGCAGGCGATTAAGGCAGGGGTAACGGTTGGGATTGGTTTTGTCGGTATGGGACTGGTGATCGTCATGGCGATCGACAGCCTCAGTCCCCCT                                       <  1:143436/115‑1
                                                                                                                                                     CCTTATCTTCCGCATCCCCTGGCTGCAGGCGATTAAGGCAGGGGTAACGGTTGGGATTGGTTTTGTCGGTATGGGACTGGTGATCGTCATGGCGATCGACAGCCTCAGTCCCCCT                                       >  2:143436/1‑115
                                                                                                                                                     CCTTATCTTCCGCATCCCCTGGCTGCAGGCGATTAAGGCAGGGGTAACGGTTGGGATTGGTTTTGTCGGTATGGGACTGGTGATCGTCATGGCGATCGACAGCCTCAGTCCCCCT                                       >  2:36721/1‑115
                                                                                                                                                     CCTTATCTTCCGCATCCCCTGGCTGCAGGCGATTAAGGCAGGGGTAACGGTTGGGATTGGTTTTGTCGGTATGGGACTGGTGATCGCCATGACGATCacca                                                     <  1:793448/101‑5
                                                                                                                                                     CCTTATCGTCCGCATCCCCTGGCTGCAGGCGATTAAGGCAGGGGTAACGGTTGGGTTTGGTTTTGTCGGTATGGGACTGGTGATCGCCATGACGATCacca                                                     >  2:793448/1‑97
                                                                                                                                                      CTTATCTTCCGCATCCCCTGGCTGCAGGCGATTAAGGCAGGGGTAACGGTTGGGATTGGTTTTGTCGGTATGGGACTGGTGATCGTCATGGCGATCGACAGCCTCAGTCCCCCTATCAAAGTGATGATTGAGCGTTTCGGCCTAGCCCT    <  1:1213111/149‑1
                                                                                                                                                      CTTATCTTCCGCATCCCCTGGCTGCAGGCGATTAAGGCAGGGGTAACGGTTGGGATTGGTTTTGTCGGTATGGGACTGGTGATCGTCATGGCGATCGACAGCCTCAGTCCCCCTATCAAAGTGATAATTGAGCGTTTCGGCCTAGCCCT    <  2:1360337/149‑1
                                                                                                                                                      CTTATCTTCCGCATCCCCTGGCTGCAGGCGATTAAGGCAGGGGTAACGGTTGGGATTGGTTTTGTCGGTATGGGACTGGTGATCGTCATGGCGATCGACAGCCTCAGTCCCCCTATCAAAGT                               <  2:1351384/122‑1
                                                                                                                                                      CTTATCTTCCGCATCCCCTGGCTGCAGGCGATTAAGGCAGGGGTAACGGTTGGGATTGGTTTTGTCGGTATGGGACTGGTGATCGTCATGGCGATCGACAGCCTCAGTCCCCCTATCAAAGT                               >  1:1351384/1‑122
                                                                                                                                                       TTATCTTCCGCATCCCCTGGCTGCAGGCGATTAAGGCAGGGGTAACGGTTGGGATTGGTTTTGTCGGTATGGGACTGGTGATCGTCATGGCGATCGACAGCCTCAGTCCCCCTATCAAAGTGATGATTGAGCGTTTCGGCCTAGCCCTG   >  3:220500/1‑149
                                                                                                                                                        TATCTTCCGCATCCCCTGGCTGCAGGCGATTAAGGCAGGGGTAACGGTTGGGATTGGTTTTGTCGGTATGGGACTGGTGATCGTCATGGCGATGGACAGCCTCAGTCCCCCTATCAAAGTGATGATTGAGCGTTTCGGCCTAGCCCTGC  <  1:1275976/149‑1
                                                                                                                                                                                                                                                                                                             
GCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4529992‑4530145
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gccttaTCTTCCGCATCCCCTGGCTGCAGGCGATTAAGGCAGGGGTAACGGTTGGGATTGGTTTTGTCGGTATGGGACTGGTGATCGTCATGGCGATCGACAGCCTCAGTCCCCCTATCAAAGTGATGATTGAGCGTTTCGGCCTAGCCCTGC  <  NC_000913/4530173‑4530027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.