breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
MC JC | 257,908 | Δ776 bp | [crl] | [crl] | |
JC JC | 1,293,015 | IS1 (+) +8 bp | intergenic (‑93/‑505) | hns ← / → tdk | global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase |
RA | 1,419,624 | G→A | intergenic (‑168/+141) | ydaG ← / ← racR | Rac prophage; uncharacterized protein/Rac prophage; putative DNA‑binding transcriptional regulator |
RA | 1,685,527 | A→G | G354G (GGT→GGC) | fumC ← | fumarate hydratase (fumarase C),aerobic Class II |
RA | 2,145,313 | C→A | S683Y (TCC→TAC) | yegE → | putative diguanylate cyclase |
RA | 2,173,363 | Δ2 bp | intergenic (‑1/+1) | gatC ← / ← gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
RA | 2,318,095 | C→G | L594F (TTG→TTC) | rcsC ← | hybrid sensory kinase in two‑component regulatory system with RcsB and YojN |
RA | 3,560,455 | +G | intergenic (‑2/+1) | glpR ← / ← glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
RA | 3,993,569 | C→T | P806L (CCG→CTG) | cyaA → | adenylate cyclase |
RA | 4,117,174 | A→C | S17A (TCC→GCC) | glpK ← | glycerol kinase |
RA | 4,296,381 | +GC | intergenic (+587/+55) | gltP → / ← yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
Unassigned missing coverage evidence | ||||||||||
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seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NC_000913 | 1978498 | 1979288 | 791 | 51 [49] | [46] 50 | insB1–insA | insB1,insA |
* | * | ÷ | NC_000913 | 2469075 | 2470620 | 1546 | 50 [45] | [49] 50 | [gtrB]–gtrS | [gtrB],gtrS |
* | * | ÷ | NC_000913 | 2994992 | 2995424 | 433 | 51 [48] | [47] 50 | [ygeK]–[ygeN] | [ygeK],[ygeN] |
Unassigned new junction evidence | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | = 1299498 | 0 (0.000) | 47 (0.370) | 37/510 | 2.7 | 100% | intergenic (+253/‑1684) | ychE/oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein |
? | NC_000913 | 1300698 = | 0 (0.000) | intergenic (+1453/‑484) | ychE/oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein | |||||
* | ? | NC_000913 | = 1978502 | 1 (0.010) | 48 (0.380) | 36/502 | 2.7 | 99.0% | intergenic (‑305/+16) | flhD/insB1 | flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B |
? | NC_000913 | 1979279 = | 0 (0.000) | intergenic (‑64/‑474) | insA/uspC | IS1 repressor TnpA/universal stress protein | |||||
* | ? | NC_000913 | 3721045 = | 166 (1.280) | 329 (2.540) | 146/516 | 0.0 | 68.4% | noncoding (413/1443 nt) | IS150 | repeat region |
? | NC_000913 | = 3839774 | 139 (1.070) | coding (220/819 nt) | nlpA | cytoplasmic membrane lipoprotein‑28 |