New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3738815 =376 (2.380)25 (0.170) 16/250 NT 7.0% coding (1319/2031 nt) malS alpha‑amylase
?NC_000913 3738835 = 314 (2.120)coding (1339/2031 nt) malS alpha‑amylase

Only 100 of 504 total aligned reads displayed.

GGCATAGCTTTAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCGCTTCGCGAATGGAAAAAAGCTAACCCCGACAAAGCATTAGATGACAAACCTTTCTGGATGACCGGTGAAGCCTGGGGCCACGGCGTGATGCAAAGTGACTACTATCGCCACGGCTTCGATGCGATGATCAATTTCGATTATCAGGAGCAGGCGGCGAAAGCAGTCGACTGT  >  NC_000913/3738569‑3739107
                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                
   aTAGCTTTAAGCATTACATTAATTTCAGCGAAAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGGTCAGAACGGTTATTGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCcgc                                                                                                                                                                                                                                                                       <  4:43056/275‑1 (MQ=255)
   aTAGCTTTAACGATTACATTAAATTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACGATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCcgc                                                                                                                                                                                                                                                                       <  2:409033/275‑1 (MQ=255)
       gtttAACGAGTACATTAATTTCGGCGACAAAACAGGCTGGGATAAATGGTGGTGAAAAAACTGGATCAGAACGGATATGCGTGATTACGACACTCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCGGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTAGACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGttaagtaa                                                                                                                                                                                                                                                                    <  4:86384/273‑8 (MQ=255)
                        aTTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGtttt                                                                                                                                                                                                                                                  <  4:54885/275‑1 (MQ=255)
                             aGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTc                                                                                                                                                                                                                                                                          >  2:58506/1‑246 (MQ=255)
                                  cAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAATCCTGGATTCGACGATCCCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACACACGCCAAAGACATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGCCAGTGGGTCCGCGACTATGGGATTGATGGTTTCCGGGTCGATa                                                                                                                                                                                                                                        >  2:390116/1‑275 (MQ=255)
                                            tGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGAGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAAc                                                                                                                                                                                                                               >  1:47694/1‑274 (MQ=255)
                                               gATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAATAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTCACCCACTGGTTAAGTCAGTGGGTCCGCCACTATGGGATTTATGGTTTTCGGGTCGATACCgtaaatca                                                                                                                                                                                                                              >  2:144804/1‑265 (MQ=255)
                                                        tggGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCCCGATTACTTAACCCACTGGTTAATTCAGTGGGTCCGCCACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTg                                                                                                                                                                                                                    >  2:504613/1‑273 (MQ=255)
                                                         ggggAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGc                                                                                                                                                                                                                   <  2:223503/273‑1 (MQ=255)
                                                                      gATCAGAACGGATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAACCCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCCGCAAc                                                                                                                                                                                                    >  4:188529/1‑275 (MQ=255)
                                                                                    tCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGc                                                                                                                                                                                       <  2:531405/274‑1 (MQ=255)
                                                                                      ggCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAg                                                                                                                                                                                    >  1:74982/1‑275 (MQ=255)
                                                                                      ggCGATTACGACAATCCTGGATTCGACGATCTCACCATGTCGCCAGCCTTTTTGCCGGTTATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAACGGATACCCCCGCCAAAGCCATTGACGGCTCTACGCCGCGCGATTACTTAACCCACTGGGTAAGTCAGTGGGTCCGCGACTTAGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTCACCGCCGGGCAGCAACTGAAAACCGAAGCCAg                                                                                                                                                                                    >  4:440042/1‑275 (MQ=255)
                                                                                                           ttCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCGCTTCGCGAATGGaa                                                                                                                                                               <  2:306802/275‑1 (MQ=255)
                                                                                                              gacgaTCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCGCTTCGCGAATGGaaaaa                                                                                                                                                            >  2:178197/1‑275 (MQ=255)
                                                                                                                             ggttaaGCCTGTTTTGCGGAGTTCAAACCCAAATCAGCTGACAGTGCTGGACTGCAGGTGTGCTATAAAAACAAAATGGATACCCACGCCAAAGCCAGTGAGGGCTATACGCCGCGGCATTACTTAACCCACGGGTTAAAGCAGTGTGTCCGCGACTAGGGGGTTGAGGGGTTTCGAATGGATACCGCCAAACAGGTTGAGTTGCCGGCCTGGCAGGAACTGGAAACCGAGGCCATGGCCGAGCTTCGCGAATGTAAAAAAGCGAACGCCGACa                                                                                                                                              <  4:299894/269‑1 (MQ=255)
                                                                                                                                                    cAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCcgc                                                                                                                                                                                                                                                                       >  6:310386/1‑130 (MQ=255)
                                                                                                                                                          cGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGt                                                                                                                                                                                                                                                                               >  6:179744/1‑116 (MQ=255)
                                                                                                                                                            aaTCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCGCTTCGCGAATGGAAAAAAGCTAACCCCGACAAAGCATTAGATGACAAACCTTTCTGGATGAca                                                                                                              >  2:374041/1‑274 (MQ=255)
                                                                                                                                                                  cTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGa                                                                                                                                                                                                                                                         >  7:876032/1‑130 (MQ=255)
                                                                                                                                                                  cTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGa                                                                                                                                                                                                                                                         <  8:876032/130‑1 (MQ=255)
                                                                                                                                                                     ccGTTTCTGGTTTGCCGGTGTTCTATAAAAACAAAATGGATCCCCCCGCCAAAGCCCTTGCCGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCGCTTCGCGAATGGAAAAAAGCTAACCCCGACAAAGCATTAGATGACAAACCTTTCTGGATGACCGGTGAAGcc                                                                                                     <  4:338164/275‑1 (MQ=255)
                                                                                                                                                                        cTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGt                                                                                                                                                                                                                                                                           >  1:108263/1‑106 (MQ=255)
                                                                                                                                                                         tgctgccaggcgggcaactcaacatgtttggcggtatcgacccgaaaaccatcaatcccatagtcgcggacccactgACTTAACCCACTGGTTAAGTc                                                                                                                                                                                                                                                                                  >  8:133646‑M1/78‑98 (MQ=255)
                                                                                                                                                                                cTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCggg                                                                                                                                                                                                                                              >  6:831152/1‑127 (MQ=255)
                                                                                                                                                                                cTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCggg                                                                                                                                                                                                                                              <  5:831152/127‑1 (MQ=255)
                                                                                                                                                                                       tgtTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGt                                                                                                                                                                                                                                                                               >  6:408395/1‑87 (MQ=255)
                                                                                                                                                                                             ataAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCg                                                                                                                                                                                                                                           >  7:147673/1‑117 (MQ=255)
                                                                                                                                                                                             ataAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGa                                                                                                                                                                                                                        <  5:157913/136‑1 (MQ=255)
                                                                                                                                                                                             ataAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGcc                                                                                                                                                                                                              <  6:603998/146‑1 (MQ=255)
                                                                                                                                                                                             ataAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGcc                                                                                                                                                                                                              >  5:603998/1‑146 (MQ=255)
                                                                                                                                                                                              taAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACAGCCAAACATGTTGAGTTNCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCGCTTCGCGAATGGAAAAAAGATAAACACGACAAAGANTTAGAAGACAAAACTTCCTGGATGACGGGGNNAGCCGCGGGCCACGCCGGGATGAAAAg                                                                              >  3:74755/1‑273 (MQ=255)
                                                                                                                                                                                               aaaaaCAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAg                                                                                                                                                                                                                                                                                <  8:151862/78‑1 (MQ=255)
                                                                                                                                                                                                 aaaCAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATgg                                                                                                                                                                                                                                                               >  6:484060/1‑93 (MQ=255)
                                                                                                                                                                                                   aCAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCGCTTCGCGAATGGAAAAAAGCTAACCCCGACAAAGCAATAGATGACAAACCTTTCAGGCTACCCGGTGAAGCCTGGGGCCACGGCGGGATGCAAAGTCacca                                                                        >  4:111931/1‑272 (MQ=255)
                                                                                                                                                                                                    cAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGtttt                                                                                                                                                                                                                                                  >  5:413364/1‑103 (MQ=255)
                                                                                                                                                                                                      aaaTGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCggg                                                                                                                                                                                                                                              >  6:1036899/1‑105 (MQ=255)
                                                                                                                                                                                                        aTGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGAt                                                                                                                                                                                                                                                        <  5:354795/93‑1 (MQ=255)
                                                                                                                                                                                                           gATACCCACGCAAAAGCCATTGACGGCTAGACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGGCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCGCTTCGCGAATGGAAAAAAGCTAACCCCGACAAAGCATTAGATGACAAACCTTTCTGGATGACCGGTGAAGCCTGGGGCCACGGCGTGATGCAAAGTGACTACTATCGCCa                                                               <  2:77727/275‑1 (MQ=255)
                                                                                                                                                                                                            aTACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGa                                                                                                                                                                                                                        >  6:1097980/1‑121 (MQ=255)
                                                                                                                                                                                                             tACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCaaa                                                                                                                                                                                                                                >  7:931604/1‑112 (MQ=255)
                                                                                                                                                                                                                 cACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGGCTGGCAGCAACTGaaaa                                                                                                                                                                                              <  8:762089/142‑1 (MQ=255)
                                                                                                                                                                                                                 cACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGaaaa                                                                                                                                                                                              >  7:762089/1‑142 (MQ=255)
                                                                                                                                                                                                                   cGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCGCTTCGCGAATGGAAAAAAGCTAACCCCGACAAAGCATTAGATGACAAACCTTTCTGGATGAcc                                                                                                              >  4:378845/1‑220 (MQ=255)
                                                                                                                                                                                                                   cGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCGCTTCGCGAATGGAAAAAAGCTAACCCCGACAAAGCATTAGATGACAAACCTTTCTGGATGACCGGTGAAGCCTGGGGCCACGGCGTGATGCAAAGTGACTACTATCGCCACGGCTTcg                                                       >  2:260251/1‑275 (MQ=255)
                                                                                                                                                                                                                   cGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCGCTTCGCGAATGGAAAAAAGCTAACCCCGACAAAGCATTAGATGACAAACCTTTCTGGATGACCGGTGAAGCCTGGGGCCACGGCGTGATGCAAAGTGACTACTATCGCCACGGCTTcg                                                       >  2:46200/1‑275 (MQ=255)
                                                                                                                                                                                                                         aGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAAcc                                                                                                                                                                                            <  5:211882/136‑1 (MQ=255)
                                                                                                                                                                                                                         aGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGcgcc                                                                                                                                                                                >  6:233199/1‑148 (MQ=255)
                                                                                                                                                                                                                              ttGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCGCTTCGCGAATGGAAAAAAGCTAACCCCGACAAAGCATTAGATGACAAACCTTTCTGGATGACCGGTGAAGCCTGGGGCCATGGCGTGATGCAAAGTGACTACTATCGCCACGGCTTCGATGCgatgacc                                            >  2:348874/1‑273 (MQ=255)
                                                                                                                                                                                                                              ttGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCGCTTCGCGAATGGAAAAAAGCTAACCCCGACAAAGCATTAGATGACAAACCTTTCTGGATGACCGGTGAAGCCTGGGGCCACGGCGTGATGCAAAGTGAACACTATCGCCCCGGCTTCGATGCgatgaga                                            >  4:495940/1‑273 (MQ=255)
                                                                                                                                                                                                                                  cGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCGCTTcgc                                                                                                                                                                       >  7:402176/1‑148 (MQ=255)
                                                                                                                                                                                                                                   ggCTATACGCCGCCGGATTACTGAACCCACTGGTTAAGTCAGTGGGTCCGCGGCTATGGGATTGGTGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCGCTTCGCGAATGGAAAAAAGCTAACCCCGACAAAGCATTAGATGACAAACCTTTCTGGATGACCGGTGAAGCCTGGGGCCACGGCGTGATGCAAAGTGACTACTATCGCCACGGCTTCGATGCGATGATCAAttt                                       <  4:266470/275‑1 (MQ=255)
                                                                                                                                                                                                                                    gCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCGCTTCGCGa                                                                                                                                                                     <  8:402176/148‑1 (MQ=255)
                                                                                                                                                                                                                                    gCTATACGCCGCGCGACTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGt                                                                                                                                                                                                                           >  5:1074237/1‑94 (MQ=255)
                                                                                                                                                                                                                                     cTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCGCTTCGCGaa                                                                                                                                                                    >  7:55948/1‑148 (MQ=255)
                                                                                                                                                                                                                                       ataCGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCGCTTCGCGa                                                                                                                                                                     <  6:727866/145‑1 (MQ=255)
                                                                                                                                                                                                                                       ataCGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCGCTTCGCGa                                                                                                                                                                     >  5:727866/1‑145 (MQ=255)
                                                                                                                                                                                                                                             cgcgcgATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCGCTTCGCGAATGGAAAAAAGCTAACCCCGACAAAGCATTAGATGACAAACCTTTCTGGATGACCGGTGAAGCCTGGGGCCACGGCGTGATGCAAAGTGACTACTATCGCCACGGCTTCGATGCGATGATCAATTTCGATTATCAg                             >  4:65536/1‑275 (MQ=255)
                                                                                                                                                                                                                                              gcgcgATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGt                                                                                                                                                                                                                      <  7:739675/89‑1 (MQ=255)
                                                                                                                                                                                                                                              gcgcgATTAATTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGt                                                                                                                                                                                                                      >  8:739675/1‑89 (MQ=255)
                                                                                                                                                                                                                                                 cgATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGa                                                                                                                                                                                          >  6:132410/1‑114 (MQ=255)
                                                                                                                                                                                                                                                   ctgacttaaccagtgggttaagtCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCaa                                                                                                                                                                                                     >  6:636254‑M2/24‑101 (MQ=255)
                                                                                                                                                                                                                                                   ctgacttaaccagtgggttaagtCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCaa                                                                                                                                                                                                     <  5:636254‑M2/78‑1 (MQ=255)
                                                                                                                                                                                                                                                   ctgacttaaccagtgggttaagtAAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCGCTTCGCGAATGGaaaaa                                                                                                                                                            >  7:666938‑M2/24‑142 (MQ=255)
                                                                                                                                                                                                                                                   ctgacttaaccagtgggttaagtAAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCGCTTCGCGAATGGaaaaa                                                                                                                                                            <  8:666938‑M2/119‑1 (MQ=255)
                                                                                                                                                                                                                                                   aTTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGa                                                                                                                                                                                          <  8:712794/112‑1 (MQ=255)
                                                                                                                                                                                                                                                       cttaaccagtgggttaagtCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCGCTTcgcg                                                                                                                                                                      <  7:707245‑M2/109‑1 (MQ=255)
                                                                                                                                                                                                                                                       CTTAGCCCACTGGTTAAGTCAGTGGGTCCGCGACTATggg                                                                                                                                                                                                                                                              >  8:1079641‑M1/1‑40 (MQ=255)
                                                                                                                                                                                                                                                       CTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATggg                                                                                                                                                                                                                                                              <  7:1079641‑M1/40‑1 (MQ=255)
                                                                                                                                                                                                                                                        TTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCGCTTCGCGAATGGAAAAAAGCTAACCCCGACAAAGCATTAGATGACAAACCTTTCTGGATGACCGGTGAAGCCTGGGGCCACGGCGTGATGCAAAGTGACTACTATCGCCACGGCTTCGATGCGATGATCAATTTCGATTATCAGGAGCAggcggc                  >  2:481527‑M1/1‑275 (MQ=255)
                                                                                                                                                                                                                                                         TAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGaaa                                                                                                                                                                                               <  7:1143993‑M1/101‑1 (MQ=255)
                                                                                                                                                                                                                                                         TAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAgcg                                                                                                                                                                                  >  8:1028998‑M1/1‑114 (MQ=255)
                                                                                                                                                                                                                                                           accagtgggttaagtCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCGCTTcg                                                                                                                                                                        <  7:361557‑M2/107‑1 (MQ=255)
                                                                                                                                                                                                                                                           accagtgggttaagtCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCGCTTcg                                                                                                                                                                        >  8:361557‑M2/16‑122 (MQ=255)
                                                                                                                                                                                                                                                              cACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGaa                                                                                                                                                                                         <  6:972106/102‑1 (MQ=255)
                                                                                                                                                                                                                                                              cACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGaa                                                                                                                                                                                         >  5:972106/1‑102 (MQ=255)
                                                                                                                                                                                                                                                              cACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCGCTTCGCGAATGGAAAAAAGCTAACCCCGACAAAg                                                                                                                                           >  7:494413/1‑148 (MQ=255)
                                                                                                                                                                                                                                                              cACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCGCTTCGCGAATGGAAAAAAGCTAACCCCGACAAAg                                                                                                                                           >  5:389922/1‑148 (MQ=255)
                                                                                                                                                                                                                                                               aCTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGa                                                                                                                                                                                          >  5:1018528/1‑100 (MQ=255)
                                                                                                                                                                                                                                                                cTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGc                                                                                                                                                                                       >  8:495093/1‑102 (MQ=255)
                                                                                                                                                                                                                                                                cTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCGCTTCGCGaa                                                                                                                                                                    >  6:90526/1‑121 (MQ=255)
                                                                                                                                                                                                                                                                cTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCGCTTCGCGaa                                                                                                                                                                    <  5:90526/121‑1 (MQ=255)
                                                                                                                                                                                                                                                                cTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCGCTTCGCGAATGGAAAAAAGCTAACCCCGACAAAGc                                                                                                                                          >  6:36952/1‑147 (MQ=255)
                                                                                                                                                                                                                                                                cTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCGCTTCGCGAATGGAAAAAAGCTAACCCCGACAAAGCa                                                                                                                                         >  7:1041977/1‑148 (MQ=255)
                                                                                                                                                                                                                                                                cTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCGCTTCGCGAATGGAAAAAAGCTAACCCCGACAAAGCa                                                                                                                                         >  6:442314/1‑148 (MQ=255)
                                                                                                                                                                                                                                                                    ttaagtCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCGCTTCGCGAATGGAAAAAAGCTAACCCCGACAAAGCATTAg                                                                                                                                     <  8:55948‑M2/142‑1 (MQ=255)
                                                                                                                                                                                                                                                                    ttaagtCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCGCTTCGCGAATGGAAAAAAGCTAACCCCGACAAAGCATTAGATGACAAACCTTTCTGGATGACCGGTGAAGCCTGGGGCCACGGCGTGATGCAAAGTGACTACTATCGCCACGGCTTCGATGCGATGATCAATTTCGATTATCAGGAGCAGGCGGCGAAAGCAGTCGa      <  2:96496‑M2/269‑1 (MQ=255)
                                                                                                                                                                                                                                                                     taagtCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCGCTTCGCGAATGGAAAAAAGCTAACCCCGACAAAGCATTAGATGACAAACCTTTCTGGATGACCGGTGAAGCCTGGGGCCACGGCGTGATGCAAAGTGACTACTATCGCCACGGCTTCGATGCGATGATCAATTTCGATTATCAGGAGCAGGCGGCGa                <  2:278925‑M2/259‑1 (MQ=255)
                                                                                                                                                                                                                                                                       agtCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCGCTTCGCGAATGGAAAAAAGCTAACCCCGACAAAGCAtt                                                                                                                                       >  7:429876‑M2/4‑143 (MQ=255)
                                                                                                                                                                                                                                                                       agtCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCGCTTCGCGAATGGAAAAAAGCTAACCCCGACAAAGCAtt                                                                                                                                       <  8:429876‑M2/140‑1 (MQ=255)
                                                                                                                                                                                                                                                                       agtCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCGCTTCGCGAATGGAAAAAAGCTAACCCCGACAAAGCATTAGATg                                                                                                                                  >  8:922314‑M2/4‑148 (MQ=255)
                                                                                                                                                                                                                                                                        gtCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCGCTTcg                                                                                                                                                                        <  5:303949‑M2/107‑1 (MQ=255)
                                                                                                                                                                                                                                                                        gtCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCGCTTCGCGAATGGAAAAAAGCTAACCCCGACACAGCATTAGATGACAAACCTTTCTGGATGACCGGTGAAGCCTGGGGCCACGGCGTGATGCGAAGTGACTACTATCGCCACGGCTTCGATGCGATCATCCATTTCGATTATCAGGGGCAGGCGTCGAAAGCAGTCCACTGt  >  2:445829‑M2/3‑275 (MQ=255)
                                                                                                                                                                                                                                                                         tCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGa                                                                                                                                                                                          >  5:120341‑M2/2‑90 (MQ=255)
                                                                                                                                                                                                                                                                          cAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGa                                                                                                                                                                                                                        <  8:255776/59‑1 (MQ=255)
                                                                                                                                                                                                                                                                          cAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGa                                                                                                                                                                                                                        >  7:255776/1‑59 (MQ=255)
                                                                                                                                                                                                                                                                          cAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGc                                                                                                                                                                                                                   <  7:814538/64‑1 (MQ=255)
                                                                                                                                                                                                                                                                          cAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGc                                                                                                                                                                                                                   >  8:814538/1‑64 (MQ=255)
                                                                                                                                                                                                                                                                          cAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGcc                                                                                                                                                                                      >  5:480565/1‑93 (MQ=255)
                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                
GGCATAGCTTTAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCGCTTCGCGAATGGAAAAAAGCTAACCCCGACAAAGCATTAGATGACAAACCTTTCTGGATGACCGGTGAAGCCTGGGGCCACGGCGTGATGCAAAGTGACTACTATCGCCACGGCTTCGATGCGATGATCAATTTCGATTATCAGGAGCAGGCGGCGAAAGCAGTCGACTGT  >  NC_000913/3738569‑3739107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.