mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | 29-END_S10_L001_R1_001_150407.good.fq | 387,539 | 68,930,194 | 100.0% | 177.9 bases | 275 bases | 97.2% |
| errors | 29-END_S10_L001_R1_001_150520.good.fq | 1,006,712 | 195,940,195 | 100.0% | 194.6 bases | 275 bases | 98.7% |
| errors | 29-END_S10_L001_R2_001_150407.good.fq | 387,539 | 71,119,020 | 100.0% | 183.5 bases | 275 bases | 74.4% |
| errors | 29-END_S10_L001_R2_001_150520.good.fq | 1,006,709 | 196,680,520 | 100.0% | 195.4 bases | 275 bases | 93.9% |
| errors | GLY_29_031_S324_L001_R1_001.good.fq | 103,774 | 14,757,560 | 100.0% | 142.2 bases | 149 bases | 98.1% |
| errors | GLY_29_031_S324_L001_R2_001.good.fq | 103,774 | 14,757,560 | 100.0% | 142.2 bases | 149 bases | 97.5% |
| errors | GLY_29_031_S324_L002_R1_001.good.fq | 101,186 | 14,362,397 | 100.0% | 141.9 bases | 149 bases | 98.0% |
| errors | GLY_29_031_S324_L002_R2_001.good.fq | 101,186 | 14,362,397 | 100.0% | 141.9 bases | 149 bases | 97.4% |
| total | 3,198,419 | 590,909,843 | 100.0% | 184.8 bases | 275 bases | 93.9% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_000913 | 4,641,652 | 110.5 | 3.6 | 100.0% | Escherichia coli str. K-12 substr. MG1655, complete genome. |
| total | 4,641,652 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 3604 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 2 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 555 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.067 |
| reference sequence | pr(no read start) |
|---|---|
| NC_000913 | 0.79812 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 0 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 6 |
| option | value |
|---|---|
| Mode | Full Polymorphism |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 2 |
| Polymorphism frequency cutoff | 0.05 |
| Polymorphism minimum variant coverage each strand | 2 |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | ≥3 bases |
| Skip base substitutions when they create a homopolymer flanked on each side by | ≥5 bases |
| program | version |
|---|---|
| bowtie2 | 2.3.4.1 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 17:03:07 02 Jan 2019 | 17:04:00 02 Jan 2019 | 53 seconds |
| Read alignment to reference genome | 17:04:00 02 Jan 2019 | 17:15:36 02 Jan 2019 | 11 minutes 36 seconds |
| Preprocessing alignments for candidate junction identification | 17:15:36 02 Jan 2019 | 17:16:20 02 Jan 2019 | 44 seconds |
| Preliminary analysis of coverage distribution | 17:16:20 02 Jan 2019 | 17:18:36 02 Jan 2019 | 2 minutes 16 seconds |
| Identifying junction candidates | 17:18:36 02 Jan 2019 | 17:18:41 02 Jan 2019 | 5 seconds |
| Re-alignment to junction candidates | 17:18:41 02 Jan 2019 | 17:21:57 02 Jan 2019 | 3 minutes 16 seconds |
| Resolving best read alignments | 17:21:57 02 Jan 2019 | 17:23:15 02 Jan 2019 | 1 minute 18 seconds |
| Creating BAM files | 17:23:15 02 Jan 2019 | 17:25:13 02 Jan 2019 | 1 minute 58 seconds |
| Tabulating error counts | 17:25:13 02 Jan 2019 | 17:26:03 02 Jan 2019 | 50 seconds |
| Re-calibrating base error rates | 17:26:03 02 Jan 2019 | 17:26:05 02 Jan 2019 | 2 seconds |
| Examining read alignment evidence | 17:26:05 02 Jan 2019 | 18:15:37 02 Jan 2019 | 49 minutes 32 seconds |
| Polymorphism statistics | 18:15:37 02 Jan 2019 | 18:15:38 02 Jan 2019 | 1 second |
| Output | 18:15:38 02 Jan 2019 | 18:15:53 02 Jan 2019 | 15 seconds |
| Total | 1 hour 12 minutes 46 seconds | ||
