New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3670807 =69 (0.670)3 (0.030) 3/252 NT 3.8% coding (1216/1650 nt) treF cytoplasmic trehalase
?NC_000913 3670847 = 86 (0.870)coding (1256/1650 nt) treF cytoplasmic trehalase
Rejected: Frequency below/above cutoff threshold.

ACAATGGCGGCAGCGGAAAACAGCGCCAGTTGTTCGCGTCGCCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3670850‑3670807
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgccaTTGTGCCACTCTATGTCGGTATGGCGAACCATGAACAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCA  >  NC_000913/3670847‑3670992
                                                                                                                                                                                              
ACAATGGCGGCCGAGGAAAACAGCGCCAGTTGTTCGCGTCGCCATGGTGCCACTCTATGTCGGTATGGCGAACCATGAACAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGT                                           >  4:665261/1‑149
   ATGGCGGCAGCGGAAAACAGCGCCAGTTGTTCGCGTCGCCATTGTGCCACTCTATGTCGGTATGGCGAACCATGAA                                                                                                                 <  1:213707/76‑1
   ATGGCGGCAGCGGAAAACAGCGCCAGTTGTTCGCGTCGCCATTGTGCCACTCTATGTCGGTATGGCGAACCATGAA                                                                                                                 >  2:213707/1‑76
                                        GCCATTGTGCCACTCTATGTCGGTATGGCGAACCATGAACAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAGACCGGTGAACAGTGGGATAAACCCAACGGCTGGGC   >  3:836851/1‑149
                                        GCCATTGTGCCACTCTATGTCGGTATGGCGAACCATGAACAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGC   >  2:195696/1‑149
                                         CCATTGTGCCACTCTATGTCGGTATGGCGAACCATGAACAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGGACAGTGGGATAAACCCAACGGCTGGGCA  >  2:606029/1‑149
                                           ATTGTGCCACTCTATGTCGGTATGGCGAACCATGAACAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTG                                                     >  3:364212/1‑96
                                           ATTGTGCCACTCTATGTCGGTATGGCGAACCATGAACAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTG                                                     <  4:364212/96‑1
                                                                                                                                                                                              
ACAATGGCGGCAGCGGAAAACAGCGCCAGTTGTTCGCGTCGCCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3670850‑3670807
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgccaTTGTGCCACTCTATGTCGGTATGGCGAACCATGAACAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCA  >  NC_000913/3670847‑3670992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.