breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errors5-216_S5_L001_R1_001_150209.good.fq414,179106,642,794100.0%257.5 bases275 bases98.3%
errors5-216_S5_L001_R1_001_150513.good.fq616,471161,617,512100.0%262.2 bases276 bases98.0%
errors5-216_S5_L001_R2_001_150209.good.fq414,179106,951,807100.0%258.2 bases275 bases86.5%
errors5-216_S5_L001_R2_001_150513.good.fq616,471162,536,698100.0%263.7 bases276 bases68.3%
total2,061,300537,748,811100.0%260.9 bases276 bases86.9%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistributionNC_0009134,641,65296.53.6100.0%Escherichia coli str. K-12 substr. MG1655, complete genome.
total4,641,652100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 1000002617
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 2
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 5000130
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.016

Junction Skew Score Calculation

reference sequencepr(no read start)
NC_0009130.87858

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 3
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 1

Read Alignment Evidence

optionvalue
ModeConsensus/Mixed Base
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoff0.75
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff10
Polymorphism frequency cutoff0.2
Polymorphism minimum variant coverage each strandOFF
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs ofOFF
Skip base substitutions when they create a homopolymer flanked on each side byOFF

Software Versions

programversion
bowtie22.3.4.1
R3.4.4

Execution Times

stepstartendelapsed
Read and reference sequence file input23:10:26 07 Jan 201923:11:07 07 Jan 201941 seconds
Read alignment to reference genome23:11:07 07 Jan 201923:25:15 07 Jan 201914 minutes 8 seconds
Preprocessing alignments for candidate junction identification23:25:15 07 Jan 201923:25:47 07 Jan 201932 seconds
Preliminary analysis of coverage distribution23:25:47 07 Jan 201923:27:42 07 Jan 20191 minute 55 seconds
Identifying junction candidates23:27:42 07 Jan 201923:27:45 07 Jan 20193 seconds
Re-alignment to junction candidates23:27:45 07 Jan 201923:30:04 07 Jan 20192 minutes 19 seconds
Resolving best read alignments23:30:04 07 Jan 201923:31:02 07 Jan 201958 seconds
Creating BAM files23:31:02 07 Jan 201923:32:41 07 Jan 20191 minute 39 seconds
Tabulating error counts23:32:41 07 Jan 201923:33:25 07 Jan 201944 seconds
Re-calibrating base error rates23:33:25 07 Jan 201923:33:26 07 Jan 20191 second
Examining read alignment evidence23:33:26 07 Jan 201923:41:26 07 Jan 20198 minutes 0 seconds
Polymorphism statistics23:41:26 07 Jan 201923:41:26 07 Jan 20190 seconds
Output23:41:26 07 Jan 201923:41:38 07 Jan 201912 seconds
Total 31 minutes 12 seconds