breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
MC JC | 257,908 | Δ776 bp | [crl] | [crl] | |
RA | 1,685,527 | A→G | G354G (GGT→GGC) | fumC ← | fumarate hydratase (fumarase C),aerobic Class II |
MC JC | 1,978,503 | Δ776 bp | insB1–insA | insB1, insA | |
RA | 2,145,313 | C→A | S683Y (TCC→TAC) | yegE → | putative diguanylate cyclase |
RA | 2,173,363 | Δ2 bp | intergenic (‑1/+1) | gatC ← / ← gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
RA | 2,318,095 | C→G | L594F (TTG→TTC) | rcsC ← | hybrid sensory kinase in two‑component regulatory system with RcsB and YojN |
JC JC | 3,173,112 | IS5 (+) +4 bp | intergenic (+1/‑27) | mdaB → / → ygiN | NADPH quinone reductase/quinol monooxygenase |
RA | 3,560,455 | +G | intergenic (‑2/+1) | glpR ← / ← glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
RA | 3,993,145 | C→A | R665S (CGT→AGT) | cyaA → | adenylate cyclase |
RA | 4,117,047 | G→T | S59Y (TCC→TAC) | glpK ← | glycerol kinase |
RA | 4,129,994 | Δ1 bp | coding (160/2433 nt) | metL → | Bifunctional aspartokinase/homoserine dehydrogenase 2 |
RA | 4,183,165 | G→A | E641K (GAA→AAA) | rpoB → | RNA polymerase, beta subunit |
RA | 4,296,381 | +GC | intergenic (+587/+55) | gltP → / ← yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
Unassigned new junction evidence | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | = 1299498 | 0 (0.000) | 80 (0.820) | 55/512 | 0.5 | 100% | intergenic (+253/‑1684) | ychE/oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein |
? | NC_000913 | 1300698 = | 0 (0.000) | intergenic (+1453/‑484) | ychE/oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein | |||||
* | ? | NC_000913 | 3721045 = | 140 (1.420) | 161 (1.640) | 96/518 | 0.0 | 54.4% | noncoding (413/1443 nt) | IS150 | repeat region |
? | NC_000913 | = 3839774 | 131 (1.330) | coding (220/819 nt) | nlpA | cytoplasmic membrane lipoprotein‑28 |