New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4611259 =102 (1.180)4 (0.050) 3/162 NT 4.2% intergenic (+256/‑137) prfC/osmY peptide chain release factor RF‑3/salt‑inducible putative ABC transporter periplasmic binding protein
?NC_000913 4611304 = 89 (1.130)intergenic (+301/‑92) prfC/osmY peptide chain release factor RF‑3/salt‑inducible putative ABC transporter periplasmic binding protein
Rejected: Frequency below cutoff threshold.

TGAGCCTGGCAACACGAGGTTGCATTGCTGAATAGGGAGAGACTTCACCCTCTACAGAAGTCAATGGTCGCCATCACAAAAGCGATGAGTGATGAATAACGACCATT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4611365‑4611259
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgaccattGACTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTA  >  NC_000913/4611304‑4611402
                                                                                                                                                                                                              
TGAGCCTGGCAACACGAGGTTGCATTGCTGAATAGGGAGAGACTTCACCCTCTACAGAAGTCAATGGTCGCCATCACAAAAGCGATGAGTGATGAATAAC                                                                                                            <  1:1280201/100‑1
   GCCTGGCAACACGAGGTTGCATTGCTGAATAGGGAGAGACTTCACCCTCTACAGAAGTCAATGGTCGCCATCACAAAAGCGATGAGTGATGAATAACGAC                                                                                                         >  1:1428155/1‑100
   GCCTGGCAACACGAGGTTGCATTGCTGAATAGGGAGAGACTTCACCCTCTACAGAAGTCAATGGTCGCCATCACAAAAGCGATGAGTGATGAATAACGAC                                                                                                         >  1:999368/1‑100
    CCTGGCAACACGAGGTTGCATTGCTGAATAGGGAGAGACTTCACCCTCTACAGAAGTCAATGGTCGCCATCACAAAAGCGATGTGTGATGAATAACGACC                                                                                                        <  2:1062252/100‑1
     CTGGCAACACGAGGTTGCATTGCTGAATAGGGAGAGACTTCACCCTCTACAGAAGTCAATGGTCGCCATCACAAAAGCGAAGAGTGATGAATAACGACCA                                                                                                       >  1:744391/1‑100
      TGGCAACACGAGGTTGCATTGCTGAATAGGGAGAGACTTCACCCTCTACAGAAGTCAATGGTCGCCATCACAAAAGCGATGAGTGATGAATAACGACCAT                                                                                                      <  2:1414013/100‑1
      TGGCAACACGAGGTTGCATTGCTGAATAGGGAGAGACTTCACCCTCTACAGAAGTCAATGGTCGCCATCACAAAAGCGATGAGTGATGAATAACGACCAT                                                                                                      >  2:1089188/1‑100
       GGCAACACGAGGTTGCATTGCTGAATAGGGAGAGACTTCACCCTCTACAGAAGTAAATGGTCGCCATCACAAAAGCGATGAGTGATGAATAACGACCATT                                                                                                     <  2:1352778/100‑1
       GGCAACACGAGGTTGCATTGCTGAATAGGGAGAGACTTCACACTCTACAGAAGTCAATGGTCGCCATCACAAAAGCGATGAGTGATGAATAACGACCATT                                                                                                     >  2:1117777/1‑100
                                                          AGTCAATGGTCGCCATCACAAAAGCGATGAGTGATGAATAACGACCATTGACTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGT                                                  >  1:778382/1‑100
                                                          AGTCAATGGTCGCCATCAAAAAAGCGATGAGTGATGAATAACGACCATTGACTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGT                                                  >  2:192245/1‑100
                                                           GTCAATGGTCGCCATCACAAAAGCGATGAGTGATGAATAACGACCATTGACTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTT                                                 >  1:1129210/1‑100
                                                              AATGGTCGCCATCACAAAAGCGATGAGTGATGAATAACGACCATTGACTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCC                                              >  1:935065/1‑100
                                                                                                   CGACCATTGACTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATGAATCG         >  2:2401141/1‑100
                                                                                                   CGACCATTGACTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCG         >  2:1855335/1‑100
                                                                                                   CGACCATTGACTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATACAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCG         <  2:874478/100‑1
                                                                                                    GACCATTGACTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGA        <  1:725885/100‑1
                                                                                                    GACCATTGACTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGA        >  2:614492/1‑100
                                                                                                     ACCATTGACTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGAT       >  1:2342765/1‑100
                                                                                                      CCATTGACTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATG      >  1:2213606/1‑100
                                                                                                      CCATTGACTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATG      <  2:2255664/100‑1
                                                                                                      CCATTGACTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATG      <  1:1114525/100‑1
                                                                                                       CATTGACTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATC          <  1:334909/95‑1
                                                                                                       CATTGACTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACACGAATAAATCGATGA     >  2:1633373/1‑100
                                                                                                        ATTGACTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGAC    <  2:2299820/100‑1
                                                                                                        ATTGACTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGAC    <  2:2300335/100‑1
                                                                                                        ATTGACTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGAC    <  1:2032749/100‑1
                                                                                                        ATTGACTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGAC    >  2:842869/1‑100
                                                                                                        ATTGACTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGAC    >  1:246968/1‑100
                                                                                                         TTGACTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACT   >  1:1917366/1‑100
                                                                                                         TTGACTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGAC    >  1:1894420/1‑99
                                                                                                          TGACTTCTGTAGATGGTGAAGTCTCTACCTATTCAGCAATGCAACCTCGTGTTGCCATTCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTA  <  2:191209/100‑1
                                                                                                          TGACTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTA  <  1:2015603/100‑1
                                                                                                          TGACTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTA  <  2:1069658/100‑1
                                                                                                          TGACTTCTGTAGAGGGTGAAGTCACTCCCTATTCCCCAATGCAACCTCGTGATGCCAGCCTAAAATTACGAGCAAACATACAGGAATAAATCGATGACTA  >  2:449776/1‑100
                                                                                                                                                                                                              
TGAGCCTGGCAACACGAGGTTGCATTGCTGAATAGGGAGAGACTTCACCCTCTACAGAAGTCAATGGTCGCCATCACAAAAGCGATGAGTGATGAATAACGACCATT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4611365‑4611259
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgaccattGACTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTA  >  NC_000913/4611304‑4611402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.