Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	4,242,942	Δ66 bp	coding (510‑575/891 nt)	malG ←	maltose transporter subunit

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	4242942	4243007	66	64 [1]	[1] 64	malG	maltose transporter subunit

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 4242941	1 (0.010)	53 (0.680)	40/184	1.5	98.2%	coding (576/891 nt)	malG	maltose transporter subunit
	?	NC_000913	4243008 =	1 (0.010)					coding (509/891 nt)	malG	maltose transporter subunit

GCCTGCCACGGTGTCGCACCATCCAGCGCAGCAGCTTCTTCCAGCGAACTGTCGATGGTTTCGAAATAGCCTTTGATGGTCCAGACATGCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCATACAACGCGACC  >  NC_000913/4242912‑4243106                                                                                                 |                                                                                                   ataaacgacaggatgaataccaccgccagaatcggtactgacagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATcc                                                                                                 <  1:1759684‑M2/4‑1 (MQ=255)    aacgacaggatgaataccaccgccagaatcggtactgacagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATcccac                                                                                              <  1:1002574‑M2/7‑1 (MQ=255)      cgacaggatgaataccaccgccagaatcggtactgacagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATcccaccc                                                                                            >  2:106836‑M2/92‑100 (MQ=255)      cgacaggatgaataccaccgccagaatcggtactgacagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATcccaccc                                                                                            >  1:628361‑M2/92‑100 (MQ=255)      cgacaggatgaataccaccgccagaatcggtactgacagcggcaacagcaccaggcggaaggcctgccacgatgccgcacgatccagcgcaATccca                                                                                               >  2:110052‑M2/92‑97 (MQ=255)           ggatgaataccaccgccagaatcggtactgacagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAg                                                                                          >  2:268991‑M2/87‑97 (MQ=255)           ggatgaataccaccgccagaatcggtactgacagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATa                                                                                       >  2:789735‑M2/87‑100 (MQ=255)           ggatgaataccaccgccagaatcggtactgacagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATa                                                                                       >  2:418910‑M2/87‑100 (MQ=255)           ggatgaataccaccgccagaatcggtactgacagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATa                                                                                       >  1:1851896‑M2/87‑100 (MQ=255)            gatgaataccaccgccagaatcggtactgacagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATAc                                                                                      <  1:1989617‑M2/15‑1 (MQ=255)             atgaataccaccgccagaatcggtactgacagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATAcg                                                                                     >  2:532492‑M2/85‑100 (MQ=255)               gaataccaccgccagaatcggtactgacagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATAcgcg                                                                                   <  2:1283526‑M2/18‑1 (MQ=255)                  taccaccgccagaatcggtactgacagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGaaa                                                                                >  2:309213‑M2/80‑100 (MQ=255)                    ccaccgccagaatcggtactgacagcggcaacaggtcaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAAt                                                                              >  1:341082‑M2/78‑100 (MQ=255)                    ccaccgccagaatcggtactgacagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGaaaa                                                                               >  1:184166‑M2/78‑99 (MQ=255)                    ccaccgccagaatcggtactgacagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAAt                                                                              <  1:495118‑M2/23‑1 (MQ=255)                    ccaccgccagaatcggtactgacagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAAt                                                                              >  1:592788‑M2/78‑100 (MQ=255)                    ccaccgccagaatcggtactgacagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAAt                                                                              <  1:1607675‑M2/23‑1 (MQ=255)                     caccgccagaatcggtactgacagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAAtt                                                                             <  2:1084666‑M2/24‑1 (MQ=255)                     caccgccagaatcggtactgacagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAAtt                                                                             >  2:1621454‑M2/77‑100 (MQ=255)                       ccgccagaatcggtactgacagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTAc                                                                           <  1:2016472‑M2/26‑1 (MQ=255)                       ccgccagaatcggtactgacagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTAc                                                                           >  1:1356740‑M2/75‑100 (MQ=255)                                 cggtactgacagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGa                                                                 >  2:1469716‑M2/65‑100 (MQ=255)                                   gtactgacagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGt                                                               >  1:547893‑M2/63‑100 (MQ=255)                                          cagcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAgg                                                        <  2:307689‑M2/45‑1 (MQ=255)                                            gcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGcc                                                      <  2:869812‑M2/47‑1 (MQ=255)                                            gcggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGcc                                                      >  1:1403147‑M2/54‑100 (MQ=255)                                             cggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCa                                                     <  2:1115598‑M2/48‑1 (MQ=255)                                             cggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCa                                                     >  2:791733‑M2/53‑100 (MQ=255)                                              ggcaacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCaa                                                    <  1:789738‑M2/49‑1 (MQ=255)                                                caacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCaatg                                                  <  1:1186197‑M2/51‑1 (MQ=255)                                                caacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCaatg                                                  <  2:1635661‑M2/51‑1 (MQ=255)                                                caacaggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCaatg                                                  <  1:160387‑M2/51‑1 (MQ=255)                                                    aggacaaggcggaaggcctgccacggtgtcgcaccatccagcgccATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCaatgaatg                                              <  2:141369‑M2/55‑1 (MQ=255)                                                     ggacaaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATgaatgg                                             >  1:1718414‑M2/45‑100 (MQ=255)                                                        caaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATgaatggaat                                          <  2:1270426‑M2/59‑1 (MQ=255)                                                        caaggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACCCGAAAATCACCCCGCCACGAGTATTCAGACCAATgaatggaa                                           >  2:678148‑M2/42‑99 (MQ=255)                                                          aggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGt                                        >  1:987350‑M2/40‑100 (MQ=255)                                                          aggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGt                                        >  2:672408‑M2/40‑100 (MQ=255)                                                          aggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGt                                        <  1:1880594‑M2/61‑1 (MQ=255)                                                          aggcggaaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGt                                        <  1:418911‑M2/61‑1 (MQ=255)                                                                aaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCAc                                  <  2:1282819‑M2/67‑1 (MQ=255)                                                                aaggcctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCAc                                  <  2:1610368‑M2/67‑1 (MQ=255)                                                                     ctgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGa                             >  1:341361‑M2/29‑100 (MQ=255)                                                                      tgccacggtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGAc                            >  2:1365647‑M2/28‑100 (MQ=255)                                                                             gtgtcgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCaaa                     <  2:987346‑M2/80‑1 (MQ=255)                                                                                 cgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAaacaac                 >  1:1108947‑M2/17‑100 (MQ=255)                                                                                 cgcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAaacaac                 <  1:672410‑M2/84‑1 (MQ=255)                                                                                  gcaccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACg                <  2:1767058‑M2/85‑1 (MQ=255)                                                                                  cAGACATGCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACg                >  1:124083/1‑100 (MQ=255)                                                                                   caccatccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGc               <  2:1356731‑M2/86‑1 (MQ=255)                                                                                      catccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGACCATTCAGGCCAATGAATGGAATGTACTCACCCAGACGAACAAACAACGCATa            >  2:6147‑M2/12‑100 (MQ=255)                                                                                       atccagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCATAc           >  2:953650‑M2/11‑100 (MQ=255)                                                                                        tccagcgcaAACCCACACAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCATACa          <  1:602971‑M2/91‑1 (MQ=255)                                                                                          cagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCATACAAc        >  2:1888056‑M2/8‑100 (MQ=255)                                                                                           agcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCATACAAcg       <  2:547891‑M2/94‑1 (MQ=255)                                                                                            gcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCATACAAcgc      >  1:1023714‑M2/6‑100 (MQ=255)                                                                                              gcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCATACAACGCGa    >  1:1581451‑M2/4‑100 (MQ=255)                                                                                              gcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCATACAACGCGa    >  1:1581447‑M2/4‑100 (MQ=255)                                                                                               caATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCATACAACGCGAc   <  2:341081‑M2/98‑1 (MQ=255)                                                                                               caATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCATACAACGCGAc   <  1:791736‑M2/98‑1 (MQ=255)                                                                                                aATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCATACAACGCGAcc  <  1:1409717‑M2/99‑1 (MQ=255)                                                                                                aATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCATACAACGCGAcc  <  2:592786‑M2/99‑1 (MQ=255)                                                                                                aATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCATACAACGCGAcc  <  2:184165‑M2/99‑1 (MQ=255)                                                                                                 |                                                                                                   GCCTGCCACGGTGTCGCACCATCCAGCGCAGCAGCTTCTTCCAGCGAACTGTCGATGGTTTCGAAATAGCCTTTGATGGTCCAGACATGCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCATACAACGCGACC  >  NC_000913/4242912‑4243106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑