New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 4615442149 (1.010)10 (0.080) 7/158 NT 6.7% coding (102/864 nt) yjjW putative pyruvate formate lyase activating enzyme
?NC_000913 = 4615456 146 (1.120)coding (88/864 nt) yjjW putative pyruvate formate lyase activating enzyme
Rejected: Frequency below cutoff threshold.

CACCACTTTGCCGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4615334‑4615442
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gtttttgcagGGCTGCAATCTGCGCTGCAAAAACTGTCACAATCCGTGGACGATGGGACGTTGCAATGACTGTGGGGAGTGCGTGCCACAGTGTCCGCATCAGGCGTTG  <  NC_000913/4615456‑4615358
                                                                                                                                                                                                                
CACCACTTTGCCGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAG                                                                                                              >  1:3984338/1‑100
  CCACTTTGCCGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTT                                                                                                            >  2:2858194/1‑100
   CACTTTGCCGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAG                                                                                                              <  1:4011200/97‑1
    ACTTTGCCGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTT                                                                                                          >  2:2427345/1‑100
     CTTTGCCGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGTAACGTCCCATCGTCCACGGATTGTGACAGTTTTT                                                                                                         <  2:1298644/100‑1
     CTTTGCCGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTT                                                                                                         <  1:2881151/100‑1
      TTTGCCGTCANNAATCTCCANCGCCNGATGCNGACACTGTTGCACGCACTCCCCACAGTCATTGCANCGTCCCATCGTCCACGGATTGTGACAGTTTTTG                                                                                                        <  1:1849310/100‑1
       TTGCCGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCTCATCGTCCACGGATTGTTACAGTTTTTGC                                                                                                       <  1:407304/100‑1
       TTGCCGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTG                                                                                                        <  2:379640/99‑1
        TGCCGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCA                                                                                                      >  1:3208167/1‑100
         GCCGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAG                                                                                                     >  1:886322/1‑100
           CGTCATCCATCTGCAACGCCTGATGCGGACACTGGGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCTTCCACGGATTGTGACAGTTTTT                                                                                                         <  2:3431958/94‑1
                            GCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGGGCTGCAATCTGCGCTGCA                                                                                  <  1:79723/100‑1
                                 ATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGGGCTGCAATCTGCGCTGCAAAAAC                                                                             <  1:1005555/100‑1
                                  TGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGGGCTGCAATCTGCGCTGCAAAAACT                                                                            <  2:2044204/100‑1
                                  TGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGGGCTGCAATCTGCGCTGCAAAAAC                                                                             <  1:4037780/99‑1
                                   GCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGGGCTGCAATCTGCGCTGCAAAAACTG                                                                           <  1:811298/100‑1
                                   GCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCAGCGTCCACGGATTGTGACAGTTTTTGCAGGGCTGCAATCTGCGCTGCAAAAACTG                                                                           <  2:3831637/100‑1
                                   GCGGACACCGTGGCACGCAGTCCCCACAGCCATTGTCACGTCCCATCGTCCACGGATTGAGACAGTTTTTGCAGGGCTGCAATCTGCGCTGCAAAAACTG                                                                           <  2:2376277/100‑1
                                     GGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGGGCTGCAATCTGCGCTGCAAAAACTGTC                                                                         <  2:671309/100‑1
                                      GACACTGTGGCACGCACTCCCCGCAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTGTTGCAGGGCTGCAATCGGCGCTGCAAAAACTGTCA                                                                        <  2:860554/100‑1
                                       ACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGGGCTGCAATCTGCGCTGCAAAAACTGTCAC                                                                       <  1:1182558/100‑1
                                                                                                   GTTTTTGCAGGGCTGCAATCTGCGCTGCAAAAACTGTCACAATCCGTGGACGATGGGACGTTGCAATGACTGTGGGGAGTGCGTGCCACAGTGTCCGCAT           >  1:2002466/1‑100
                                                                                                    TTTTTGCAGGGCTGCAATCTGCGCTGCAAAAACTGTCACAATCCGTGGACGATGGGACGTTGCAATGACTGTGGGGAGTGCGTGCCACAGTGTCCGCATC          >  1:2890338/1‑100
                                                                                                     TTTTGCAGGGCTGCAATCTGCGCTGCAAAAACTGTCACCATCCGTGGACGATGGGACGTTGCAATGACTGTGGGGAGTGCGTGCCACAGTGTCCGCATCA         >  2:1265322/1‑100
                                                                                                     TTTTGCAGGGCTGCAATCTGCGCTGCAAAAACTGTCACAATCCGTGGACGATGGGACGTTGCAATGACTGTGGGGAGTGCGTGCCACAGTGTCCGCATCA         <  1:961460/100‑1
                                                                                                     TTTTGCAGGGCTGCAATCTGCGCTGCAAAAACTGTCACAATCCGTGGACGATGGGACGTTGCAATGACTGTGGGGAGTGCGTGCCACAGTGTCCGCATCA         >  1:3444001/1‑100
                                                                                                     TTTTGCAGGGCTGCAATCTGCGCTGCAAAAACTGTCACAATCCGTGGACGATGGGACGTTGCAATGACTGTGGGGAGTGCGTGCCACAGTGTCCGCATCA         >  1:2866407/1‑100
                                                                                                      TTTGCAGGGCTGCAATCTGCGCTGCAAAAACTGTCACAATCCGTGGACGATGGGACGTTGCAATGACTGTGGGGAGTGCGTGCCACAGTGTCCGCATCAG        <  1:90323/100‑1
                                                                                                      TTTGCAGGGCTGCAATCTGCGCTGCAAAAACTGTCACAATCCGTGGACGATGGGACGTTGCAATGACTGTGGGGAGTGCGTGCCACAGTGTCCGCATCAG        >  2:2510655/1‑100
                                                                                                      TTTGCAGGGCTGCAATCTGCGCTGCAAAAACTGTCACAATCCGTGGACGATGGGACGTTGCAATGACTGTGGGGAGTGCGTGCCACAGTGTCCGCATCAG        >  1:1395847/1‑100
                                                                                                       TTGCAGGGCTGCAATCTGCGCTGCAAAAACTGTCACAAGCCGTGGACGATGGGACGTTGCAATGACCGGGGGGAGTGCGTGCCACAGTGTCCGCATCAGG       >  2:2544518/1‑100
                                                                                                        TGCAGGGCTGCAATCTGCGCTGCAAAAACTGTCACAATCCGTGGACGATGGGACGTTGCAATGACTGTGGGGAGTGCGTGCCACAGTGTCCGCATCAGGC      >  1:2429835/1‑100
                                                                                                        TGCAGGGCTGCAATCTGCGCTGCAAAAACTGTCACAATCCGTGGACGATGGGACGTTGCAATGACTGTGGGGAGTGCGTGCCACAGTGTCCGCATCAGGC      <  2:987005/100‑1
                                                                                                          CAGGGCTGCAATCTGCGCTGCAAAAACTGTCACAATCCGTGGACGATGGGACGTTGCAATGACTGTGGGGAGTGCGTGCCACAGTGTCCGCATCAGGCGT    >  1:2858210/1‑100
                                                                                                            GGGCTGCAATCTGCGCTGCAAAAACTGTCACAATCCGTGGACGATGGGACGTTGCAATGACTGTGGGGAGTGCGTGCCACAGTGTCCGCATCAGGCGTTG  <  2:1439986/100‑1
                                                                                                            GGGCTGCAATCTGCGCTGCAAAAACTGTCACAATCCGTGGACGATGGGACGTTGCAATGACTGTGGGGAGTGCGTGCCACAGTGTCCGCATCAGGCGTTG  >  1:2164290/1‑100
                                                                                                            GGGCTGCAATCTGCGCTGCAAAAACTGTCACAATCCGTGGACGATGGGACGTTGCAATGACTGTGGGGAGTGCGTGCCACAGTGTCCGCATCAGGCGTTG  <  2:791684/100‑1
                                                                                                                                                                                                                
CACCACTTTGCCGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4615334‑4615442
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gtttttgcagGGCTGCAATCTGCGCTGCAAAAACTGTCACAATCCGTGGACGATGGGACGTTGCAATGACTGTGGGGAGTGCGTGCCACAGTGTCCGCATCAGGCGTTG  <  NC_000913/4615456‑4615358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.