mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.29.0 revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | qtrim-IND_KHP_HOT_A3_F110_I0_R1_S20_L004_R1_001 | 2,356,877 | 235,610,329 | 100.0% | 100.0 bases | 100 bases | 97.3% |
| errors | qtrim-IND_KHP_HOT_A3_F110_I0_R1_S20_L004_R2_001 | 2,356,855 | 235,065,851 | 100.0% | 99.7 bases | 100 bases | 96.1% |
| total | 4,713,732 | 470,676,180 | 100.0% | 99.9 bases | 100 bases | 96.7% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_000913 | 4,641,652 | 98.0 | 2.0 | 98.1% | Escherichia coli str. K-12 substr. MG1655, complete genome. |
| total | 4,641,652 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 100000 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 3 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 1986 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.089 |
| reference sequence | pr(no read start) |
|---|---|
| NC_000913 | 0.65070 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 0 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 6 |
| option | value |
|---|---|
| Mode | Full Polymorphism |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | OFF |
| Consensus minimum coverage each strand | OFF |
| Polymorphism E-value cutoff | 2 |
| Polymorphism frequency cutoff | 0.15 |
| Polymorphism minimum coverage each strand | 2 |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | ≥3 bases |
| Skip base substitutions when they create a homopolymer flanked on each side by | ≥2 bases |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 19:05:33 14 Jan 2017 | 19:06:21 14 Jan 2017 | 48 seconds |
| Read alignment to reference genome | 19:06:22 14 Jan 2017 | 19:08:34 14 Jan 2017 | 2 minutes 12 seconds |
| Preprocessing alignments for candidate junction identification | 19:08:34 14 Jan 2017 | 19:10:15 14 Jan 2017 | 1 minute 41 seconds |
| Preliminary analysis of coverage distribution | 19:10:15 14 Jan 2017 | 19:12:07 14 Jan 2017 | 1 minute 52 seconds |
| Identifying junction candidates | 19:12:07 14 Jan 2017 | 19:35:16 14 Jan 2017 | 23 minutes 9 seconds |
| Re-alignment to junction candidates | 19:35:16 14 Jan 2017 | 19:35:48 14 Jan 2017 | 32 seconds |
| Resolving alignments with junction candidates | 19:35:48 14 Jan 2017 | 19:38:40 14 Jan 2017 | 2 minutes 52 seconds |
| Creating BAM files | 19:38:40 14 Jan 2017 | 19:39:45 14 Jan 2017 | 1 minute 5 seconds |
| Tabulating error counts | 19:39:45 14 Jan 2017 | 19:41:46 14 Jan 2017 | 2 minutes 1 second |
| Re-calibrating base error rates | 19:41:46 14 Jan 2017 | 19:41:47 14 Jan 2017 | 1 second |
| Examining read alignment evidence | 19:41:47 14 Jan 2017 | 20:27:11 14 Jan 2017 | 45 minutes 24 seconds |
| Polymorphism statistics | 20:27:11 14 Jan 2017 | 20:27:13 14 Jan 2017 | 2 seconds |
| Output | 20:27:13 14 Jan 2017 | 20:29:17 14 Jan 2017 | 2 minutes 4 seconds |
| Total | 1 hour 23 minutes 43 seconds | ||
