New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	4389174 =	64 (0.750)	10 (0.130)	5/164	NT	16.3%	coding (197/3324 nt)	mscM	mechanosensitive channel protein, miniconductance
	?	NC_000913	4389211 =	44 (0.560)					coding (160/3324 nt)	mscM	mechanosensitive channel protein, miniconductance

CTGGAGCAGGCAAAAGCGGCGAAACCCGCACAGCCGGAAGTCGTAGAGGCGCTCCAGTCTGCCTTAAATGCGCTTGAGGAACGAAAAGGTTCCCTTGAGCGCAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4389277‑4389174 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑agcgcatTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGC  >  NC_000913/4389211‑4389307                                                                                                                                                                                                           CTGGAGCAGGCAAAAGCGGCGAAACCCGCACAGCCGGAAGTCGTAGAGGCGCTCCAGTCTGCCTTAAATGCGCTTGAGGAACGAAAAGGTTCCCTTGAGC                                                                                                       <  1:732715/100‑1   GGAGCAGGCAAAAGCGGCGAAACCCGCACAGCCGGAAGTCGTAGAGGCGCTCCAGTCTGCCTTAAATGCGCTTGAGGAACGAAAAGGTTCCCTTGAGCGC                                                                                                     >  2:1285031/1‑100   GGAGCAGGCAAAAGCGGCGAAACCCGCACAGCCGGAAGTCGTAGAGGCGCTCCAGTCTGCCTTAAATGCGCTTGAGGAACGAAAAGGTTCCCTTGAGCGC                                                                                                     >  2:1739231/1‑100   GGAGCAGGCAAAAGCGGCGAAACCCGCACAGCCGGAAGTCGTAGAGGCGCTCCAGTCTGCCTTAAATGCGCTTGAGGAACGAAAAGGTTCCCTTGAGCG                                                                                                      <  2:1474266/99‑1     AGCAGGCAAAAGCGGCGAAACCCGCACAGCCGGAAGTCGTAGAGGCGCTCCAGTCTGCCTTAAATGCGCTTGAGGAACGAAAAGGTTCCCTTGAGCGCAT                                                                                                   <  2:561693/100‑1      GCAGGCAAAAGCGGCGAAACCCGCACAGCCTGAAGTCGTAGAGGCGCTCCAGTCTGCCTTAACTGCGCTTGAGGAACGAAAAGGTTCCCTTGAGC                                                                                                       <  2:2275748/95‑1                                                            TGCCTTAAATGCGCTTGAGGAACGAAAAGGTTCCCTTGAGCGCATTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTT                                            >  1:1387155/1‑100                                                              CCTTAAATGCGCTTGAGGAACGAAAAGGTTCCCTTGAGCGCATTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTG                                          >  2:337943/1‑100                                                              CCTTAAATGCGCTTGAGGAACGAAAAGGTTCCCTTGAGCGCATTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTG                                          >  1:533553/1‑100                                                              CCTTAAATGCGCTTGAGGAACGAAAAGGTTCCCTTGAGCGCATTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTG                                          >  1:870289/1‑100                                                              CCTTAAATGCGCTTGAGGAACGAAAAGGTTCCCTTGAGCGCATTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTG                                          >  1:936036/1‑100                                                              CCTTAAATGCGCTTGAGGAACGAAAAGGTTCCCTTGAGCGCATTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTG                                          >  2:2030791/1‑100                                                               CTTAAATACGCTTGAGGAACGAAAAGGTTCCCTTGAGCGCATTTAAGGCAGACTGGAGCGCCTCTGCGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGC                                         >  2:1252373/1‑100                                                                TTAAATGCGCTTGAGGAACGAAAAGGTTCCCTTGAGCGCATTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCC                                        >  1:852726/1‑100                                                                TTAAATGCGCTTGAGGAACGAAAAGGTTCCCTTGAGCGCATTTAAGGCAGACTGGAGCGCCTCTACGACATCCGGCTGTGCGGGTTTCACCGCTTTTGC                                         >  2:822403/1‑99                                                                        GCTTGAGGAACGAAAAGGTTCCCTTGAGCGCATTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAG                                <  1:337945/100‑1                                                                                                  AGCGCATTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGG      >  2:1542630/1‑100                                                                                                  AGCGCATTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGG      >  1:262957/1‑100                                                                                                     GCATTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGG   <  1:231564/100‑1                                                                                                      CATTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGC  <  2:1787851/100‑1                                                                                                                                                                                                           CTGGAGCAGGCAAAAGCGGCGAAACCCGCACAGCCGGAAGTCGTAGAGGCGCTCCAGTCTGCCTTAAATGCGCTTGAGGAACGAAAAGGTTCCCTTGAGCGCAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4389277‑4389174 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑agcgcatTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGC  >  NC_000913/4389211‑4389307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.