Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	380,020	(G)10→7	intergenic (‑139/+47)	frmR ← / ← yaiO	regulator protein that represses frmRAB operon/outer membrane protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	380016 =	2 (0.030)	65 (0.940)	49/184	0.5	97.1%	intergenic (‑135/+53)	frmR/yaiO	regulator protein that represses frmRAB operon/outer membrane protein
	?	NC_000913	= 380012	2 (0.030)					intergenic (‑131/+57)	frmR/yaiO	regulator protein that represses frmRAB operon/outer membrane protein

GATGATTTTAACGGTCTGCAACTTGCAGCCCGTCTGACCTGGAAATTCTGATTCCTTCTGCCGCCCGCTATCCGGGGCGGCCTTCCCTGCCGATTAGCCCCCCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/380119‑380016 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cccccccTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTTATATACTATAGGGGGGTATGCATTGACATATAGAATACCCCCCTA  <  NC_000913/380012‑379914                                                                                                                                                                                                             GATGATTTTAACGGTCTGCAACTTGCAGCCCGTCTGACCTGGAAATTCTGATTCCTTCTGCCGCCCGCTATCCGGAGCGGCCTTCCCTGCCGATTAGCC                                                                                                          >  2:1131551/1‑99     ATTTTAACGGTCTGCAACTTGCAGCCCGTCTGACCTGGAAATTCTGATTCCTTCTGCCGCCCGCTATCCGGGGCGGCCTTCCCTGCCGATTAGCCCCCCC                                                                                                     >  2:926133/1‑100          AACGGTCTGCAACTTGCAGCCCGTCTGACCTGGAAATTCTGATTCCTTCTGCCGCCCGCTATCCGGGGCGGCCTTCCCTGCCGATTAGCCCCCCCTTTCC                                                                                                <  2:711189/100‑1          AACGGTCTGCAACTTGCAGCCCGTCTGACCTGGAAATTCTGATTCCTTCTGCCGCCCGCTATCCGGGGCGGCCTTCCCTGCCGATTAGCCCCCCCTTTCC                                                                                                <  2:1161078/100‑1          AACGGTCTGCAACTTGCAGCCCGCCTGACCTGGAAATTCTGATTCCTTCTGCCGCCCGCTATCCGGGGCGGCCTTCCCTGCCGATTAGCCCCCCCTTTCC                                                                                                <  2:190781/100‑1                CTGCAACTTGCAGCCCGTCTGACCTGGAAATTCTGATTCCTTTTGCCGCCCGCTATCCGGGGCGGCTTTCCCTGCCGATTAGTTTCCTCTTTCCTCTTTG                                                                                          <  1:35736/100‑1                 taCAACTTGCAGCCCGTCTGACCTGGAAATTCTGATTCCTTCTGCCGCCCGCTATCCGGGGCGGCCTTCCCTGCCGATTAGCCCCCCCTTTCCTCTct                                                                                           >  2:1234109/3‑96                 TGCAACTTGCAGCCCGTCTGACCTGGAAATTCTGATTCCTTCTGCCGCCCGCTATCCGGGGCGGCCTTCCCTGCCGATTAGCCCCCCCTTTCCTCTTTGT                                                                                         >  1:19541/1‑100                     ACTTGCAGCCCGTCTGACCTGGAAATTCTGATTCCTTCTGCCGCCCGCTATCCGGGGCCGCCTTCCCTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTC                                                                                     <  1:994289/100‑1                        TGCAGCCCGTCTGACCTGGAAATTCTGATTCCTTCTGCCGCCCGCTATCCGGGGCGGCCTTCCCTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGA                                                                                  <  2:1112491/100‑1                         GCAGCCCGTCTGACCTGGAAATTCTGATTCCTTCTGCCGCCCGCTATCCGGGGCGGCCTTCCCTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGAC                                                                                 >  2:1273728/1‑100                         GCAGCCCGTCTGACCTGGAAATTCTGATTCCTTCTGCCGCCCGCTATCCGGGGCGGCCTTCCCTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGAC                                                                                 <  1:1296538/100‑1                          CAGCCCGTCTGACCTGGAAATTCTGATTCCTTCTGCCGCCCGCTATCCGGGGCGGCCTTCCCTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACC                                                                                <  2:1148906/100‑1                             CCCGTCTGACCTGGAAATTCTGATTCCTTCTGCCGCCCGCTATCCGGGGCGGCCTTCCCTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACA                                                                             <  2:327432/100‑1                                GTCTGACCTGGAAATTCTGATTCCTTCTGCCGCCCGCTATCCGGGGCGGCCTTCCCTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTC                                                                          <  1:1364784/100‑1                                    GACCTGGAAATTCTGATTCCTTCTGCCGCCCGCTATCCGGGGCGGCCTTCCCTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCG                                                                      >  2:1684715/1‑100                                    GACCTGGAAATTCTGATTCCTTCTGCCGCCCGCTATCCGGGGCGGCCTTCCCTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCG                                                                      <  2:1484988/100‑1                                     ACCTGGAAATTCTGATTCCTTCTGCCGCCCGCTATCCGGGGCGGCCTTCCCTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGG                                                                     <  1:492755/100‑1                                             ATTCTGATTCCTTCTGCCGCCCGCTATTCGGGGCGGCCTTCCCTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTT                                                             <  2:748992/100‑1                                             ATTCTGAGTCCTTCTGCCGCCCGCTATCCGGGGCGGCCTTCCCTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTT                                                             <  1:1704253/100‑1                                              TTCTGATTCCTTCTGCCGCCCGCTATCCGGGGCGGCCTTCCCTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTT                                                            <  2:282747/100‑1                                              TTCTGATTCCTTCTGCCGCCCGCTATCCGGGGCGGCCTTCCCTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTT                                                            >  2:198698/1‑100                                               TCTGATTCCTTCTGCCGCCCGCTATCCGGGGCGGCCTTCCCTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTA                                                           >  2:34742/1‑100                                                CTGATTCCTTCTGCCGCCCGCTATCCGGGGCGGCCTTCCCTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTAT                                                          <  2:1098010/100‑1                                                 TGATTCCTTCTGCCGCCCGCTATCCGGGGCGGCCTTCCCTGCCGATTAGCCCTCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATT                                                         <  1:223613/100‑1                                                     TCCTTCTGCCGCCCGCTATCCGGGGCGGCCTTCCGTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCn                                                     <  1:956559/100‑2                                                         TCTGCCGCCCGCTATCCGGGGCGGCCTTCCCTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCA                                                    >  2:433152/1‑97                                                          CTGCCGCCCGCTATCCGGGGCGGCCTTCCCTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCAGT                                                  >  1:1778060/1‑98                                                           TGCCGCCCGCTATCCGGGGCGGCCTTCCCTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTT                                               >  1:897832/1‑100                                                            GCCGCCCGCTATCCGGGGCGGCCTTCCCTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTTA                                              >  2:244455/1‑100                                                             CCGCCCGCTATCCGGGGCGGCCTTCCCTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTTAT                                             >  2:141732/1‑100                                                             CCGCCCGCTATCCGGGGCGGCCTTCCCTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTTAT                                             >  1:760343/1‑100                                                             CCGCCCGCTATCCGGGGCGGCCTTCCCTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTTAT                                             <  1:449972/100‑1                                                              CGCCCGCTATCCGGGGCGGCCTTCCCTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTTATA                                            >  2:358139/1‑100                                                                CCCGCTATCCGGGGCGGCCTTCCCTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTTATATA                                          >  1:30590/1‑100                                                                CCCGCTATCCGGGGCGGCCTTCCCTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTTATATA                                          >  1:1597915/1‑100                                                                    CTATCCGGGGCGGCCTTCCCTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTTTTATACTAT                                      <  1:271302/100‑1                                                                    CTATCCGGGGCGGCCTTCCCTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTTATATACTAT                                      >  1:2588/1‑100                                                                    CTATCCGGGGCGGCCTTCCCTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTTATATACTAT                                      >  1:1448395/1‑100                                                                     TATCCGGGGCGGTTTTCCCTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTTA                                              <  2:248211/91‑1                                                                         CGGGGCGGCCTTCCCTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTTATATACTATAGGGG                                 <  2:1375573/100‑1                                                                           GGGCGGCCTTCCCTGCCGATTAGCGCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTTATATACTATAGGGGGG                               <  1:67473/100‑1                                                                           GGGCGGCCTTCCCTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAACTTCTATTCTCCAGTGTTATATACTATAGGGGGG                               <  1:1197264/100‑1                                                                           GGGCGGCCTTCCCTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTTATATACTATAGGGGGG                               <  1:839369/100‑1                                                                           GGGCGGCCTTACCTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAACTTATATTCTCCAGTGTTATATACTATAGGGGGG                               <  1:1197258/100‑1                                                                            GGCGGCCTTCCCTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATCTTATTCTCCAGTGTTATATACTATAGCGGGGT                              >  2:903159/1‑100                                                                             tccgatcTCCCTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTTATATACTATAGGGGGGTA                             <  2:1610637/93‑1                                                                             GCGGCCTTCCCTGCCGATTAGCCCCCCCTTTCGTCTTTGGTTCCCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTTATATACTATAGGGGGGTA                             <  1:1273717/100‑1                                                                             GCGGCCTTCCCTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTTATATACTATAGGGGGGTA                             >  2:725816/1‑100                                                                              CGGCCTTCCCTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTTATATACTATAGGGGGGTAT                            <  1:1704657/100‑1                                                                               GGCCTTCCCTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTTATATACTATAGGGGGGTATG                           >  1:742334/1‑100                                                                                GCCTTCCCTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTTATATACTATAGGGGGGTATGC                          >  1:1331375/1‑100                                                                                  ccgatCTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTTATATACTATAGGGGGGTATGCAT                        <  1:490419/95‑1                                                                                  CTTCCCTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTTATATACTATAGGGGGGTATtcta                        >  2:1653013/1‑96                                                                                     CCCTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTTATATACTATAGGGGGGTAagatcgga                     >  1:1610626/1‑92                                                                                       CTGCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTTATATACTATAGGGGGGTATGCATTGACA                   <  1:1052999/100‑1                                                                                         GCCGATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTTATATACTATAGGGGGGTATGCATAGAtcgg                 >  2:490422/1‑96                                                                                           CGATTAGCCCCCCCTTTCCTCTTTGTTTTACGACCCCATTCACCGGATAAATTTTATTCTCCAGTGTTATATACTATAGGGGGGTATGCATTGACATATA               <  1:97818/100‑1                                                                                            GATTAGCCCCTCCTTTCCTCTTTGTTTTTCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTTATATACTATAGGGGGGTATGCATTGACATATAG              <  2:516169/100‑1                                                                                            GATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTTATATACTATAGGGGGGTATGCATTGACATATAG              >  1:1779970/1‑100                                                                                             ATTAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTTATATACTATAGGGGGGTATGCATTGACATATAGA             <  2:980252/100‑1                                                                                               TAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTTATATACTATAGGGGGGTATGCATTGACATATAGAAT           <  1:612625/100‑1                                                                                               TAGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTTATATACTATAGGGGGGTATGCATTGACATATAGAAT           <  1:1234098/100‑1                                                                                                AGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTTATATACTATAGGGGGGTATGCATTGACATATAGAATA          <  2:2588/100‑1                                                                                                AGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTTATATACTATAGGGGGGTATGCATTGACATATAGAATA          >  2:770518/1‑100                                                                                                AGCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTTATATACTATAGGGGGGTATGCATTGACATATAGAATA          >  2:1481123/1‑100                                                                                                 GCCCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTTATATACTATAGGGGGGTATGCATTGACATATAGAATAC         <  2:1235422/100‑1                                                                                                   CCCCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTTATATACTATAGGGGGGTATGCATTGACATATAGAATACCC       <  2:1779983/100‑1                                                                                                    CCCTCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTTATATACTATAGGGGGGTATGCATTGACATATAGAATACCCC      <  2:785373/100‑1                                                                                                     CCCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTTATATACTATAGGGGGGTATGCATTGACATATAGAATACCCC      <  2:951447/99‑1                                                                                                      CCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTTATATACTATAGGGGGGTATGCATTGACATATAGAATACCCCCC    <  1:34742/100‑1                                                                                                      CCCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTTATATACTATAGGGGGGTATGCATTGACATATAGAATACCCCCC    <  2:720197/100‑1                                                                                                       CCTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTTATATACTATAGGGGGGTATGCATTGACATATAGAATACCCCCCT   <  1:753044/100‑1                                                                                                        CTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTTATATACTATAGGGGGGTATGCATTGACATATAGAATACCCCCCTA  <  2:81239/100‑1                                                                                                                                                                                                             GATGATTTTAACGGTCTGCAACTTGCAGCCCGTCTGACCTGGAAATTCTGATTCCTTCTGCCGCCCGCTATCCGGGGCGGCCTTCCCTGCCGATTAGCCCCCCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/380119‑380016 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cccccccTTTCCTCTTTGTTTTCCGACCACATTCACCGGATAAATTTTATTCTCCAGTGTTATATACTATAGGGGGGTATGCATTGACATATAGAATACCCCCCTA  <  NC_000913/380012‑379914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.