breseq version 0.26.1
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
MC JC | 257,908 | Δ776 bp | [crl] | [crl] | |
RA | 697,151 | A→G | intergenic (‑18/+362) | metT ← / ← asnB | tRNA‑Met/asparagine synthetase B |
RA | 757,875 | G→A | D63N (GAC→AAC) | sdhB → | succinate dehydrogenase, FeS subunit |
RA | 1,196,220 | C→T | H366H (CAC→CAT) | icd → | isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase |
RA | 1,196,232 | C→T | T370T (ACC→ACT) | icd → | isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase |
RA | 1,196,245 | T→C | L375L (TTA→CTA) | icd → | isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase |
RA | 1,196,247 | A→G | L375L (TTA→TTG) | icd → | isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase |
MC JC | 1,674,993 | 1365 bp→81 bp | coding (4‑1368/1389 nt) | pntB ← | pyridine nucleotide transhydrogenase, beta subunit |
MC JC | 1,978,503 | Δ776 bp | insB1–insA | insB1, insA | |
RA | 2,173,363 | Δ2 bp | intergenic (‑1/+1) | gatC ← / ← gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
RA | 3,053,438 | C→G | L27L (CTG→CTC) | ubiH ← | 2‑octaprenyl‑6‑methoxyphenol hydroxylase, FAD/NAD(P)‑binding |
RA | 3,475,761 | A→C | I227S (ATC→AGC) | yheO ← | putative PAS domain‑containing DNA‑binding transcriptional regulator |
RA | 3,560,455 | +G | intergenic (‑2/+1) | glpR ← / ← glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
RA | 3,815,813 | A→G | intergenic (‑45/+21) | pyrE ← / ← rph | orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH |
MC JC | 3,915,584 | 6827 bp→81 bp | [atpC]–[atpI] | [atpC], atpD, atpG, atpA, atpH, atpF, atpE, atpB, [atpI] | |
RA | 4,240,372 | G→T | L16F (TTG→TTT) | psiE → | phosphate starvation inducible protein |
RA | 4,296,381 | +GC | intergenic (+587/+55) | gltP → / ← yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
RA | 4,360,235 | G→T | intergenic (‑204/+161) | cadB ← / ← cadC | putative lysine/cadaverine transporter/cadBA operon transcriptional activator |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NC_000913 | 1166036 | 1167412 | 1377 | 21 [20] | [20] 21 | ndh | ndh |
* | * | ÷ | NC_000913 | 1196256 | 1211488 | 15233 | 21 [20] | [20] 21 | [icd]–[icdC] | 25 genes [icd], ymfD, ymfE, lit, intE, xisE, ymfI, ymfJ, cohE, croE, ymfL, ymfM, oweE, aaaE, ymfR, beeE, jayE, ymfQ, stfP, tfaP, tfaE, stfE, pinE, mcrA, [icdC] |
* | * | ÷ | NC_000913 | 3423742–3424234 | 3424532–3424239 | 6–791 | 21 [20] | [20] 21 | [rrfD]–[rrlD] | [rrfD], [rrlD] |
Unassigned new junction evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | = 1299498 | 0 (0.000) | 56 (1.180) | 52/410 | 0.0 | 100% | intergenic (+253/‑1684) | ychE/oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein |
? | NC_000913 | 1300698 = | 0 (0.000) | intergenic (+1453/‑484) | ychE/oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein |