Predicted mutation
evidence seq id position mutation annotation gene description
MC JC DH1 2,443,520 Δ23,060 bp ynaE[ttcA] 33 genes

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ DH1 2443520 2466579 23060 510 [3] [4] 511 ynaE–[ttcA] 33 genes
ynaE, pinR, tfaR, stfR, ECDH1_RS12030, ECDH1_RS12035, ECDH1_RS12040, insH1, insH1, ECDH1_RS12055, ECDH1_RS12060, ECDH1_RS12065, ynaK, trkG, ECDH1_RS12080, ECDH1_RS12085, ydaV, ydaU, ydaT, ydaS, racR, ydaF, sieB, kil, ydaE, racC, recE, recT, lar, ydaC, ydaQ, intR, [ttcA]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? DH1 = 24435193 (0.010)403 (0.990) 182/248 0.1 99.3% intergenic (+226/‑735) uspF/ynaE universal stress protein F/cold‑shock protein
?DH1 2466580 = 3 (0.010)coding (51/936 nt) ttcA tRNA 2‑thiocytidine(32) synthetase TtcA

Only 100 of 510 total aligned reads displayed.

CCATACACGCCACAGTTTTTTATATCAGATTTCTTATGCTGGGCGTTCCGGCATGACGCATACTCTTCTGATGCCATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAGTAAGGGCAAAAATCACAACTCTCTGACTCATAAGTATTTTACTTATTTTTCAATGTGTTAAATATCATCAGCGACAACAATAAGCTACGATAATCCACTCTTTTTTTGCCCCTTTTTTGCCCCTTTTGCAGCGTTTTGCCCCATT  >  DH1/2443370‑2443665
                                                                                                                                                     |                                                                                                                                                  
ccATACACGCCACAGTTTTTTATATCAGATTTCTTATGCTGGGCGTTCCGGCATGACGCATACTCTTCTGATGCCATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAA                                                                                                                                                    >  8:1793250‑M1/1‑150 (MQ=255)
 cATACACGCCACAGTTTTTTATATCAGATTTCTTATGCTGGGCGTTCCGGCATGACGCATACTCTTCTGATGCCATATAACGAATTGAGTCGCTTTTAAATGTCGCATAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAat                                                                                                                                                  <  6:920319‑M1/151‑3 (MQ=255)
 cATACACGCCACAGTTTTTTATATCAGATTTCTTATGCTGGGCGTTCCGGCATGACGCATACTCTTCTGATGCCATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAat                                                                                                                                                  <  7:1905385‑M1/151‑3 (MQ=255)
 cATACACGCCACAGTTTTTTATATCAGATTTCTTATGCTGGGCGTTCCGGCATGACGCATACTCTTCTGATGCCATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAat                                                                                                                                                  >  4:1022763‑M1/1‑149 (MQ=255)
    acacGCCACAGTTTTTTATATCAGATTTCTTATGCTGGGCGTTCCGGCATGACGCATACTCTTCTGATGCCATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAatta                                                                                                                                                <  1:88204‑M1/150‑5 (MQ=255)
    acacGCCACAGTTTTTTATATCAGATTTCTTATGCTGGGCGTTCCGGCATGACGCATACTCTTCTGATGCCATATAACGAATTGAGTCGCTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattac                                                                                                                                               >  1:77393‑M1/1‑145 (MQ=255)
     aacgCCACAGTTTTTTATATCAGATTTCTTATGCTGGGCGTTCCGGCATGACGCATACTCTTCTGATGCCATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattaca                                                                                                                                              <  3:1651421‑M1/150‑7 (MQ=255)
      acGCCACAGTTTTTTATATCAGATTTCTTATGCTGGGCGTTCCGGCATGACGCATACTCTTCTGATGCCATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaa                                                                                                                                             <  1:1014779‑M1/151‑8 (MQ=255)
        gCCACAGTTTTTTATATCAGATTTCTTATGCTGGGCGTTCCGGCATGACGCATACTCTTCTGATGCCATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaa                                                                                                                                           <  7:1273108‑M1/151‑10 (MQ=255)
         ccACAGTTTTTTATATCAGATTTCTTATGCTGGGCGTTCCGGCATGACGCATACTCTTCTGATGCCATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaa                                                                                                                                           >  3:1393856‑M1/1‑141 (MQ=255)
         ccACAGTTTTTTATATCAGATTTCTTATGCTGGGCGTTCCGGCATGACGCATACTCTTCTGATGCCATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaa                                                                                                                                           >  8:769243‑M1/1‑141 (MQ=255)
           acaGTTTTTTATATCAGATTTCTTATGCTGGGCGTTCCGGCATGACGCATACTCTTATGATGCAATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacg                                                                                                                                        >  3:202664‑M1/1‑139 (MQ=255)
             aGTTTTTTATATCAGATTTCTTATGCTGGGCGTTCCGGCATGACGCATACTCTTCTGATGCCATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtc                                                                                                                                      <  4:468597‑M1/151‑15 (MQ=255)
             aGTTTTTTATATCAGATTTCTTATGCTGGGCGTTCCGGCATGACGCATACTCTTCTGATGCCATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtc                                                                                                                                      <  4:971713‑M1/151‑15 (MQ=255)
              gTTTTTTATATCAGATTTCTTATGCTGGGCGTTCCGGCATGACGCATACTCTTCTGATGCCATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtct                                                                                                                                     <  4:913774‑M1/151‑16 (MQ=255)
               ttttttATATCAGATTTCTTATGCTGGGCGTTCCGGCATGACGCATACTCTTCTGATGCCATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctg                                                                                                                                    >  5:1893541‑M1/1‑135 (MQ=255)
                tttttATATCAGATTTCTTATGCTGGGCGTTCCGGCATGACGCATACTCTTCTGATGCCATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctg                                                                                                                                    <  2:104191‑M1/150‑17 (MQ=255)
                  tttATATCAGATTTCTTATGCTGGGCGTTCCGGCATGACGCATACTCTTCTGATGCCATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgt                                                                                                                                 >  7:654956‑M1/1‑132 (MQ=255)
                  tttATATCAGATTTCTTATGCTGGGCGTTCCGGCATGACGCATACCCTTCTGATGCCATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcg                                                                                                                                  <  5:1170863‑M1/150‑19 (MQ=255)
                      tatCAGATTTCTTATGCTGGGCGTTCCGGCATGACGCATACTCTTCTGATGCCATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgt                                                                                                                              >  7:966824‑M1/1‑128 (MQ=255)
                         cAGATTTCTTATGCTGGGCGTTCCGGCATGACGCATACTCTTCTGATGCCATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaac                                                                                                                           <  5:1711456‑M1/150‑26 (MQ=255)
                           gATTTCTTATGCTGGGCGTTCCGGCATGACGCATACTCTTCTGATGCCATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtg                                                                                                                        <  1:1639374‑M1/151‑29 (MQ=255)
                              ttCTTATGCTGGGCGTTCCGGCATGACGCATACTCTTCTGATGCCATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggc                                                                                                                     >  1:542359‑M1/1‑120 (MQ=255)
                               tCTTATGCTGGGCGTTCCGGCATGACGCATACTCTTCTGATGCCATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcg                                                                                                                    >  2:119434‑M1/1‑119 (MQ=255)
                               tCTTATGCTGGGCGTTCCGGCATGACGCATACTCTTCTGATGCCATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcg                                                                                                                    >  3:537158‑M1/1‑119 (MQ=255)
                               tCTTATGCTGGGCGTTCCGGCATGACGCATACTCTTCTGATGCCATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcg                                                                                                                    >  7:27219‑M1/1‑119 (MQ=255)
                                   aTGCTGGGCGTTCCGGCATGACGCATACTCTTCTGATGCCATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagc                                                                                                                >  6:1283982‑M1/1‑115 (MQ=255)
                                     gCTGGGGGTTCCGGCATGACGCATACTCTTCTGATGCCATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagcca                                                                                                              <  8:1112937‑M1/151‑39 (MQ=255)
                                     gCTGGGCGTTCCGGCATGACGCATACTCTTCTGATGCCATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagcc                                                                                                               <  2:1127827‑M1/150‑38 (MQ=255)
                                     gCTGGGCGTTCCGGCATGACGCATACTCTTCTGATGCCATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagcc                                                                                                               <  4:1436620‑M1/150‑38 (MQ=255)
                                       tGGGCGTTCCGGCATGACGCATACTCTTCTGATGCCATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccatt                                                                                                            >  7:1749071‑M1/1‑111 (MQ=255)
                                          gCGTTCCGGCATGACGCATACTCTTCTGATGCCATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggctaagccattgct                                                                                                         <  8:541620‑M1/151‑44 (MQ=255)
                                          gCGTTCCGGCATGACGCATACTCTTCTGATGCCATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgct                                                                                                         <  7:737675‑M1/151‑44 (MQ=255)
                                            gTTCCGGCATGACGCATACTCTTCTGATGCCATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctg                                                                                                        >  1:1782069‑M1/1‑106 (MQ=255)
                                              tCCGGCATGACGCATACTCTTCTGATGCCATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgac                                                                                                      <  1:1539665‑M1/150‑47 (MQ=255)
                                                  gCATGACGCATACTCTTCTGATGCCATATAACGAATTGAGTCGCTTTTAAATGACGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttca                                                                                                  >  4:1297169‑M1/1‑100 (MQ=255)
                                                   cATGACGCATACTCTTCTGATGCCATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaat                                                                                                >  5:1450581‑M1/1‑99 (MQ=255)
                                                          cATACTCTTCTGATGCCATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattg                                                                                         <  6:1105227‑M1/151‑60 (MQ=255)
                                                             aCTCTTCTGATGCCATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaac                                                                                      >  7:103986‑M1/1‑89 (MQ=255)
                                                                  tctGATGCCATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaagg                                                                                  <  4:1526925‑M1/150‑67 (MQ=255)
                                                                  tctGATGCCATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaagg                                                                                  <  4:788351‑M1/150‑67 (MQ=255)
                                                                     gATGCCATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcga                                                                               <  1:1229453‑M1/150‑70 (MQ=255)
                                                                      aTGCCATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacttctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatc                                                                             <  2:426532‑M1/151‑72 (MQ=255)
                                                                       tGCCATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcg                                                                            >  7:1431131‑M1/1‑79 (MQ=255)
                                                                         ccATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttctatatgattgaagaaggcgatcgc                                                                           >  1:1488384‑M1/1‑77 (MQ=255)
                                                                          cATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcat                                                                         >  8:1585455‑M1/1‑76 (MQ=255)
                                                                             ataACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcat                                                                      >  3:816451‑M1/1‑73 (MQ=255)
                                                                              taACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatg                                                                     >  8:650445‑M1/1‑72 (MQ=255)
                                                                              taACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacggctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatg                                                                     >  5:326464‑M1/1‑72 (MQ=255)
                                                                               aaCGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatg                                                                     <  4:363470‑M1/150‑80 (MQ=255)
                                                                               aaCGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatg                                                                     <  1:1125017‑M1/150‑80 (MQ=255)
                                                                               aaCGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatg                                                                     <  7:504040‑M1/150‑80 (MQ=255)
                                                                                 cGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtt                                                                  >  4:231133‑M1/1‑69 (MQ=255)
                                                                                 cGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtt                                                                  <  7:1206170‑M1/151‑83 (MQ=255)
                                                                                 cGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtt                                                                  <  2:1862736‑M1/151‑83 (MQ=255)
                                                                                 cGAATTGAGTCGATTTTAAATGTCGCAAAATAAAGAAATTAGTAAGAAAGAACAATACAACCTTAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtt                                                                  <  6:1365311‑M1/151‑83 (MQ=255)
                                                                                  gAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATAAAACCTGAACAAattacaaaaacgtctgcgtcgtaacgagggcgaagccattgcagacttaaatatgaatgaataaggcgataccatcatctttt                                                                 >  2:159068‑M1/1‑68 (MQ=255)
                                                                                   aaTTAAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACGATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtttg                                                                >  2:1292250‑M1/1‑67 (MQ=255)
                                                                                    aTTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgacgccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtttgc                                                               >  3:396792‑M1/1‑66 (MQ=255)
                                                                                     ttGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtttgcc                                                              >  3:1205897‑M1/1‑65 (MQ=255)
                                                                                           cGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtttgcctctccg                                                        <  3:1111406‑M1/151‑93 (MQ=255)
                                                                                            gCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtttgcctctccgg                                                       >  4:1732060‑M1/1‑58 (MQ=255)
                                                                                            gCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtttgcctctccgg                                                       <  2:717836‑M1/151‑94 (MQ=255)
                                                                                               tttAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtttgcctctccggggg                                                    >  1:1316518‑M1/1‑55 (MQ=255)
                                                                                               tttAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatagtttgcctctccggggg                                                    >  7:1829052‑M1/1‑55 (MQ=255)
                                                                                                 tAAATGTCGCATAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtttgcctctccgggggt                                                   <  6:1917324‑M1/150‑98 (MQ=255)
                                                                                                 tAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtttgcctctccgggggt                                                   >  1:1084983‑M1/1‑53 (MQ=255)
                                                                                                  aaaTGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtttgcctctccgggggta                                                  >  6:703446‑M1/1‑52 (MQ=255)
                                                                                                   aaTGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtttgcctctccgggggtaaa                                                <  5:582621‑M1/151‑101 (MQ=255)
                                                                                                      gTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtttgcctctccgggggtaaagac                                             >  7:790409‑M1/1‑48 (MQ=255)
                                                                                                        cGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtttgcctctccgggggtaaagac                                             >  5:702232‑M1/1‑46 (MQ=255)
                                                                                                         gCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtttgcctctccgggggtaaagacagc                                          >  5:981682‑M1/1‑45 (MQ=255)
                                                                                                         gCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtttgcctctccgggggtaaagacagc                                          <  3:181420‑M1/151‑107 (MQ=255)
                                                                                                           aaaaTCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtttgcctctccgggggtaaagacagct                                         >  3:746624‑M1/1‑43 (MQ=255)
                                                                                                            aaaTCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtttgcctctccgggggtaaagacagctat                                       <  2:1923424‑M1/151‑110 (MQ=255)
                                                                                                                  aGAAATTAGTAAGAAAGAACAATACAACCTGAACCAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtttgcctctccgggggtaaagacagctataccatg                                 >  5:1592996‑M1/1‑36 (MQ=255)
                                                                                                                  aGAAATTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtttgcctctccgggggtaaagacagctataccatg                                 >  8:261119‑M1/1‑36 (MQ=255)
                                                                                                                    aaaTTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtttgcctctccgggggtaaagacagctataccctgct                               >  4:743949‑M1/1‑34 (MQ=255)
                                                                                                                    aaaTTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtttgcctctccgggggtaaagacagctataccatgct                               >  4:1815027‑M1/1‑34 (MQ=255)
                                                                                                                     aaTTAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtttgcctctccgggggtaaagacagctataccatgctg                              >  5:1306834‑M1/1‑33 (MQ=255)
                                                                                                                       ttAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtttgactctccgggggtaaagacagctataccatgctgg                             >  2:28757‑M1/1‑31 (MQ=255)
                                                                                                                        tAGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtttgcctctccgggggtaaagacagctataccatgctgg                             >  4:1656956‑M1/1‑30 (MQ=255)
                                                                                                                         aGTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtttgcctctccgggggtaaagacagctataccatgctgg                             >  5:1125281‑M1/1‑29 (MQ=255)
                                                                                                                          gTAAGAAAGAACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtttgcctctccgggggtaaagacagctataccatgctgg                             >  6:182650‑M1/1‑28 (MQ=255)
                                                                                                                            aagaaagaACAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtttgcctctccgggggtaaagacagctataccatgctggagattc                       >  2:759043‑M1/1‑26 (MQ=255)
                                                                                                                               aaagaaCAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtttgcctctccgggggtaaagacagctataccatgctggagattctgc                    <  2:1497093‑M1/151‑129 (MQ=255)
                                                                                                                                  gaaCAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtttgcctctccgggggtaaagacagctataccatgctggagattctgcgca                 <  2:1158082‑M1/151‑132 (MQ=255)
                                                                                                                                   aaCAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtttgcctctccgggggtaaagacagctataccatgctggagattctgcgcaa                <  7:1532112‑M1/151‑133 (MQ=255)
                                                                                                                                   aaCAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtttgcctctccgggggtaaagacagctataccatgctggagattctgcgca                 >  8:1318229‑M1/1‑19 (MQ=255)
                                                                                                                                    aCAATACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtttgcctctccgggggtaaagacagctataccatgctggagattctgcgcaat               >  4:1149840‑M1/1‑18 (MQ=255)
                                                                                                                                      aaTACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtttgcctctccgggggtaaagacagctataccatgctggagattctgcgcaattt             <  5:849060‑M1/151‑136 (MQ=255)
                                                                                                                                       aTACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtttgcctctccgggggtaaagacagctataccatgctggagattctgcgcaatttg            >  8:1676367‑M1/1‑15 (MQ=255)
                                                                                                                                       aTACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtttgcctctccgggggtaaagacagctataccatgctggagattctgcgcaatttg            >  6:471658‑M1/1‑15 (MQ=255)
                                                                                                                                       aTACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcaatcgcatcatggtttgcctctccgggggtaaagacagctataccatgctggagattctgcgcaatttg            <  1:1912283‑M1/151‑137 (MQ=255)
                                                                                                                                       aTACAACCTGAACAAattacaaaaacgtctgcgtcgtaacgtcggcgaagccattgctcacttcaatatgattgaagaaggcgatcgcatcatggtttccctctcagggggtaaagacagctataccatgctggagattctgcgcaattat            >  6:1565127‑M1/1‑15 (MQ=255)
                                                                                                                                           aaCCTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtttgcctctccgggggtaaagacagctataccatgctggagattctgcgcaatttgcagc        <  5:498427‑M1/151‑141 (MQ=255)
                                                                                                                                              cTGAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtttgcctctccgggggtaaagacagctataccatgctggagattctgcgcaatttgcagaaa      >  1:680118‑M1/1‑8 (MQ=255)
                                                                                                                                                gAACAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtttgcctctccgggggtaaagacagctataccatgctggagattctgcgcaatttgcagcaaagc   <  6:979283‑M1/151‑146 (MQ=255)
                                                                                                                                                 aaCAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtttgcctctccgggggtaaagacagctataccatgctggagattctgcgcaatttgcagcaaagcg  <  2:918955‑M1/151‑147 (MQ=255)
                                                                                                                                                 aaCAAattacaaaaacgtctgcgtcgtaacgtgggcgaagccattgctgacttcaatatgattgaagaaggcgatcgcatcatggtttgcctctccgggggtaaagacagctataccatgctggagattctgcgcaatttgcagcaaagcg  <  3:951265‑M1/151‑147 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CCATACACGCCACAGTTTTTTATATCAGATTTCTTATGCTGGGCGTTCCGGCATGACGCATACTCTTCTGATGCCATATAACGAATTGAGTCGCTTTTAAATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAGTAAGGGCAAAAATCACAACTCTCTGACTCATAAGTATTTTACTTATTTTTCAATGTGTTAAATATCATCAGCGACAACAATAAGCTACGATAATCCACTCTTTTTTTGCCCCTTTTTTGCCCCTTTTGCAGCGTTTTGCCCCATT  >  DH1/2443370‑2443665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: