Predicted mutation
evidence seq id position mutation annotation gene description
MC JC DH1 2,443,520 Δ23,060 bp ynaE[ttcA] 33 genes

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ DH1 2443520 2466579 23060 510 [3] [4] 511 ynaE–[ttcA] 33 genes
ynaE, pinR, tfaR, stfR, ECDH1_RS12030, ECDH1_RS12035, ECDH1_RS12040, insH1, insH1, ECDH1_RS12055, ECDH1_RS12060, ECDH1_RS12065, ynaK, trkG, ECDH1_RS12080, ECDH1_RS12085, ydaV, ydaU, ydaT, ydaS, racR, ydaF, sieB, kil, ydaE, racC, recE, recT, lar, ydaC, ydaQ, intR, [ttcA]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? DH1 = 24435193 (0.010)403 (0.990) 182/248 0.1 99.3% intergenic (+226/‑735) uspF/ynaE universal stress protein F/cold‑shock protein
?DH1 2466580 = 3 (0.010)coding (51/936 nt) ttcA tRNA 2‑thiocytidine(32) synthetase TtcA

Only 100 of 511 total aligned reads displayed.

TGACATGAACGACGAACAGATAGCCATGTTGAATGCGCGGTTATCGTAGTTGCAAAGTTTGCCCCCAATTTGCCCCATTTAGTACCAGAGAACTGAAATAATGCAAGAAAATCAACAAATTACAAAGAAAGAACAATACAACCTGAACAAATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCGGGGGTAAAGACAGCTATACCATGCTGGAGATTCTGCGCAATTTGCAGCAAAGCGCGCC  >  DH1/2466430‑2466729
                                                                                                                                                      |                                                                                                                                                     
ccatacacgccacagttttttatatcagatttcttatgctgggcgttccggcatgacgcatactcttctgatgccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaa                                                                                                                                                       <  3:1312667‑M2/1‑1 (MQ=255)
     cacgccacagttttttatatcagatttcttatgctgggcgttccggcatgacgcatactcttctgatgccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTAc                                                                                                                                                   >  3:179662‑M2/146‑150 (MQ=255)
      acgccacagttttttatatcagatttcttatgctgggcgttccggcatgacgcatactcttctgatgccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACaa                                                                                                                                                 <  1:1014779‑M2/7‑1 (MQ=255)
        gccacagttttttatatcagatttcttatgctgggcgttccggcatgacgcatactcttctgatgccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACaaaa                                                                                                                                               <  8:1630176‑M2/9‑1 (MQ=255)
          cacagttttttatatcagatttcttatgctgggcgttccggcatgacgcatactcttctgatgccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAAc                                                                                                                                             <  7:751309‑M2/11‑1 (MQ=255)
              gttttttatatcagatttcttatgctgggcgttccggcatgacgcatactcttctgatgccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCt                                                                                                                                         >  7:525818‑M2/137‑151 (MQ=255)
                  tttatatcagatttcttatgctgggcgttccggcatgacgcatactcttctgatgccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGcgt                                                                                                                                     >  5:1749553‑M2/133‑151 (MQ=255)
                    tatatcagatttcttatgctgggcgttccggcatgacgcatactcttctgatgccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGcgtc                                                                                                                                    <  3:578777‑M2/20‑1 (MQ=255)
                    tatatcagatttcttatgctgggcgttccggcatgacgcatactcttctgatgccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGcgtc                                                                                                                                    <  4:1661143‑M2/20‑1 (MQ=255)
                     atatcagatttcttatgctgggcgttccggcatgacgcatactcttctgatgccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGcgtcg                                                                                                                                   <  1:1441591‑M2/21‑1 (MQ=255)
                       atcagatttcttatgctgggcgttccggcatgacgcatactcttctgatgccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTa                                                                                                                                 <  7:1924721‑M2/23‑1 (MQ=255)
                        tcagatttcttatgctgggcgttccggcatgacgcatactcttctgatgccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAAc                                                                                                                               >  3:793231‑M2/127‑151 (MQ=255)
                          agatttcttatgctgggcgttccggcatgacgcatactcttctgatgccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGt                                                                                                                             >  3:1910390‑M2/125‑151 (MQ=255)
                             tttcttatgctgggcgttccggcatgacgcatactcttctgatgccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTggg                                                                                                                          >  1:651619‑M2/122‑151 (MQ=255)
                             tttcttatgctgggcgttcaggcatgacgcataatattctgatgccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaataaaaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTggg                                                                                                                          >  6:941719‑M2/122‑151 (MQ=255)
                              ttcttatgctgggcgttccggcatgacgcatactcttctgatgccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTggg                                                                                                                          <  4:420255‑M2/30‑1 (MQ=255)
                               tcttatgctgggcgttccggcatgacgcatactcttctgatgccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCg                                                                                                                        >  2:1086323‑M2/120‑151 (MQ=255)
                                cttatgctgggcgttccggcatgacgcatactcttctgatgccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCg                                                                                                                        >  4:609543‑M2/119‑150 (MQ=255)
                                 ttatgctgggcgttccggcatgacgcatactcttctgatgccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGa                                                                                                                       >  4:949925‑M2/118‑150 (MQ=255)
                                 ttatgctgggcgttccggcatgacgcatactcttctgatgccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGa                                                                                                                       >  6:511554‑M2/118‑150 (MQ=255)
                                  tatgctgggcgttccggcatgacgcatactcttctgatgccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCg                                                                                                                        <  7:1767669‑M2/32‑1 (MQ=255)
                                         ggcgttccggcatgacgcatactcttctgatgccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGc                                                                                                              <  4:1071697‑M2/42‑1 (MQ=255)
                                            gttccggcatgacgcatactcttctgatgccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGa                                                                                                           <  4:12048‑M2/45‑1 (MQ=255)
                                              tccggcatgacgcatactcttctgatgccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGAc                                                                                                          <  2:284053‑M2/46‑1 (MQ=255)
                                               ccggcatgacgcatactcttctgatgccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACtt                                                                                                        <  6:438573‑M2/48‑1 (MQ=255)
                                                    atgacgcatactcttctgatgccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAat                                                                                                    >  8:519779‑M2/99‑150 (MQ=255)
                                                     tgacgcatactcttctgatgccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAatat                                                                                                  >  6:1037604‑M2/98‑151 (MQ=255)
                                                     tgacgcatactcttctgatgccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAatat                                                                                                  >  8:1473517‑M2/98‑151 (MQ=255)
                                                          catactcttctgatgccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTg                                                                                             >  3:878789‑M2/93‑151 (MQ=255)
                                                              ctcttctgatgccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctcaacaaATTACAAAAACCTCTGCCTCGTAACGTGCGCGAAGCCATTGCTGACTTCAATATGATTga                                                                                            >  1:873376‑M2/89‑148 (MQ=255)
                                                               tcttctgatgccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCAGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTgaaga                                                                                         >  8:1716710‑M2/88‑150 (MQ=255)
                                                                cttctgatgccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTgaaga                                                                                         >  7:1135751‑M2/87‑149 (MQ=255)
                                                                  tctgatgccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACATAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGaagaag                                                                                       <  5:1355066‑M2/65‑1 (MQ=255)
                                                                  tctgatgccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGaagaag                                                                                       <  1:1779449‑M2/65‑1 (MQ=255)
                                                                  tctgatgccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAgg                                                                                      <  1:1521915‑M2/66‑1 (MQ=255)
                                                                  tctgatgccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAgg                                                                                      <  7:126285‑M2/66‑1 (MQ=255)
                                                                    tgatgccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCg                                                                                    >  8:21386‑M2/83‑150 (MQ=255)
                                                                     gatgccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGc                                                                                     <  4:333522‑M2/67‑1 (MQ=255)
                                                                     gatgccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGc                                                                                     <  5:1671359‑M2/67‑1 (MQ=255)
                                                                     gatgccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGa                                                                                   <  1:1229453‑M2/69‑1 (MQ=255)
                                                                     gatgccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGAt                                                                                  >  3:157336‑M2/82‑151 (MQ=255)
                                                                     gatgccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGAt                                                                                  >  7:1843129‑M2/82‑151 (MQ=255)
                                                                         ccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCCTTGCTGACTTCAATATGATTGAAGACGGCGATCGc                                                                               >  5:1265274‑M2/78‑150 (MQ=255)
                                                                         ccatataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGca                                                                              <  1:442732‑M2/74‑1 (MQ=255)
                                                                            aTTTAGTACCAGAGAACTGAAATAATGCAAGAAAATCAACAAATTACAAAGAAAGAACAATACAACCTGAACAAATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGcatc                                                                            >  1:952049/1‑150 (MQ=255)
                                                                             ataacgaattgagtcgcttttaaatgtcgcaaaatcaagaaataagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGcatcat                                                                          >  5:198713‑M2/74‑151 (MQ=255)
                                                                               aacgaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATg                                                                         <  1:1125017‑M2/79‑1 (MQ=255)
                                                                                  gaattgagtctcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGttt                                                                     <  7:1371137‑M2/83‑1 (MQ=255)
                                                                                  gaattgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGttt                                                                     >  2:896550‑M2/69‑151 (MQ=255)
                                                                                     ttgagtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGcc                                                                  >  3:1205897‑M2/66‑151 (MQ=255)
                                                                                        agtcgcttttaaatgtcgcaaaatcaagaaattagtaagaaacaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCctct                                                               <  5:1148839‑M2/89‑1 (MQ=255)
                                                                                          tcgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTcc                                                             >  7:334242‑M2/61‑151 (MQ=255)
                                                                                           cgcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCg                                                            <  3:1111406‑M2/92‑1 (MQ=255)
                                                                                            gcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCgg                                                           <  2:717836‑M2/93‑1 (MQ=255)
                                                                                            gcttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCgg                                                           >  4:757363‑M2/59‑151 (MQ=255)
                                                                                             cttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCggg                                                          >  7:898850‑M2/58‑151 (MQ=255)
                                                                                             cttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCggg                                                          >  8:754734‑M2/58‑151 (MQ=255)
                                                                                             cttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCggg                                                          >  4:1451631‑M2/58‑151 (MQ=255)
                                                                                             cttttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGGAACCTGGGCGACGCCATTGCTGACTTCAATAAGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCggg                                                          >  6:869544‑M2/58‑151 (MQ=255)
                                                                                               tttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCggggg                                                        >  1:1316518‑M2/56‑151 (MQ=255)
                                                                                               tttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCggggg                                                        >  1:767825‑M2/56‑151 (MQ=255)
                                                                                               tttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCggggg                                                        >  7:694893‑M2/56‑151 (MQ=255)
                                                                                                ttaaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCGGGGGt                                                       >  4:83486‑M2/55‑151 (MQ=255)
                                                                                                aataatGCAAGAAAATCAACAAATTACAAAGAAAGAACAATACAACCTGAACAAATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCGGGGGt                                                       <  3:472447/151‑1 (MQ=255)
                                                                                                 taaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCGGGGGt                                                       >  1:1084983‑M2/54‑150 (MQ=255)
                                                                                                 taaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCGGGGGt                                                       <  4:1592336‑M2/97‑1 (MQ=255)
                                                                                                  aaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCGGGGGTa                                                      <  3:373756‑M2/98‑1 (MQ=255)
                                                                                                  aaatgtcgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCGGGGGTa                                                      <  5:651426‑M2/98‑1 (MQ=255)
                                                                                                        cgcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCGGGGGTAAAGACa                                                <  5:52062‑M2/104‑1 (MQ=255)
                                                                                                         gcaaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCGGGGGTAAAGACAGc                                              >  2:1146823‑M2/46‑151 (MQ=255)
                                                                                                           aaaatcaagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCGGGGGTAAAGACAGCt                                             >  2:1286701‑M2/44‑150 (MQ=255)
                                                                                                                caagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCGGGGGTAAAGACAGCTATACCa                                       <  2:190855‑M2/113‑1 (MQ=255)
                                                                                                                 aagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCGGGGGTAAAGACAGCTATACCAt                                      >  2:1554341‑M2/38‑151 (MQ=255)
                                                                                                                 aagaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCGGGGGTAAAGACAGCTATACCAt                                      >  8:625106‑M2/38‑151 (MQ=255)
                                                                                                                  agaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCGGGGGTAAAGACAGCTATACCATg                                     >  6:1236002‑M2/37‑151 (MQ=255)
                                                                                                                  agaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCGGGGGTAAAGACAGCTATACCATg                                     <  6:1447844‑M2/115‑1 (MQ=255)
                                                                                                                  agaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCCATCGCATCATGGTTTGCCACTCAGGGGGTAAAGACAGCTATACCATg                                     >  5:923604‑M2/37‑151 (MQ=255)
                                                                                                                   gaaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCGGGGGTAAAGACAGCTATACCATGc                                    <  7:1469584‑M2/116‑1 (MQ=255)
                                                                                                                    aaattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCGGGGGTAAAGACAGCTATACCATGCt                                   <  7:1590987‑M2/117‑1 (MQ=255)
                                                                                                                     aattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCGGGGGTAAAGACAGCTATACCATGCTg                                  <  8:101849‑M2/118‑1 (MQ=255)
                                                                                                                     aattagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGGCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCGGGGGTAAAGACAGCTATACCATGCTg                                  >  2:1494989‑M2/34‑151 (MQ=255)
                                                                                                                        tagtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCGGGGGTAAAGACAGCTATACCATGCTGga                                <  6:1867121‑M2/120‑1 (MQ=255)
                                                                                                                         agtaagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCGGGGGTAAAGACAGCTATACCATGCTGgag                               <  2:399213‑M2/121‑1 (MQ=255)
                                                                                                                            aagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCGGGGGTAAAGACAGCTATACCATGCTGGAGATTc                           >  2:759043‑M2/27‑151 (MQ=255)
                                                                                                                            aagaaagaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCGGGGGTAAAGACAGCTATACCATGCTGGAGATTc                           >  4:1858594‑M2/27‑151 (MQ=255)
                                                                                                                                 agaacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCGGGGGTAAAGACAGCTATACCATGCTGGAGATTCTgcgc                      <  2:1821958‑M2/130‑1 (MQ=255)
                                                                                                                                  gcacaatacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCGGGGGTAAAGACAGCTATACCATGCTGGAGATTCtgcgcc                     <  5:814800‑M2/131‑2 (MQ=255)
                                                                                                                                   aacaatacaaccttaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGGGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCGGGGGTAAAGACAGCTATACCATGCTGGAGATTCTGCGCaa                    <  2:1189138‑M2/132‑1 (MQ=255)
                                                                                                                                       atacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCGGGGGTAAAGACAGCTATACCATGCTGGAGATTCTGCGCAATTTg                <  7:1019784‑M2/136‑1 (MQ=255)
                                                                                                                                       atacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCGGGGGTAAAGACAGCTATACCATGCTGGAGATTCTGCGCAATTTg                <  5:1521823‑M2/136‑1 (MQ=255)
                                                                                                                                       atacaacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCGGGGGTAAAGACAGCTATACCATGCTGGAGATTCTGCGCAATTTg                <  5:1021856‑M2/136‑1 (MQ=255)
                                                                                                                                          caacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCGGGGGTAAAGACAGCTATACCATGCTGGAGATTCTGCGCAATTTgcag             <  5:395958‑M2/139‑1 (MQ=255)
                                                                                                                                          caacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCGGGGGTAAAGACAGCTATACCATGCTGGAGATTCTGCGCAATTTgc               >  6:1670490‑M2/13‑149 (MQ=255)
                                                                                                                                           aacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCGGGGGTAAAGACAGCTATACCATGCTGGAGATTCTGCGCAATTTgcagc            >  4:175505‑M2/12‑151 (MQ=255)
                                                                                                                                           aacctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCGGGGGTAAAGACAGCTATACCATGCTGGAGATTCTGCGCAATTTgcagc            >  8:1167961‑M2/12‑151 (MQ=255)
                                                                                                                                              ctgaacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCGGGGGTAAAGACAGCTATACCATGCTGGAGATTCTGCGCAATTTGCAGCaa          >  1:1628291‑M2/9‑150 (MQ=255)
                                                                                                                                                gaacaaATTACAAAAACGTGTGCTTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCGGGGGTAAAGACAGCTATACCATGCTGGAGATTCTGCGCAATTTGCAGCAAAg        <  4:129744‑M2/144‑1 (MQ=255)
                                                                                                                                                 aacaaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCGGGGGTAAAGACAGCTATACCATGCTGGAGATTCTGCGCAATTTGCAGCAAAgc       <  6:1330105‑M2/145‑1 (MQ=255)
                                                                                                                                                   caaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCGGGGGTAAAGACAGCTATACCATGCTGGAGATTCTGCGCAATTTGCAGCAAAgcgcg    >  3:967855‑M2/4‑151 (MQ=255)
                                                                                                                                                   caaATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCGGGGGTAAAGACAGCTATACCATGCTGGAGATTCTGCGCAATTTGCAGCAAAgcgcg    >  1:189311‑M2/4‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
TGACATGAACGACGAACAGATAGCCATGTTGAATGCGCGGTTATCGTAGTTGCAAAGTTTGCCCCCAATTTGCCCCATTTAGTACCAGAGAACTGAAATAATGCAAGAAAATCAACAAATTACAAAGAAAGAACAATACAACCTGAACAAATTACAAAAACGTCTGCGTCGTAACGTGGGCGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCGGGGGTAAAGACAGCTATACCATGCTGGAGATTCTGCGCAATTTGCAGCAAAGCGCGCC  >  DH1/2466430‑2466729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: