Predicted mutation
evidence seq id position mutation annotation gene description
MC JC DH1 2,204,804 Δ120 bp intergenic (‑239/‑54) tqsA ← / → mdtJ AI‑2 transporter TqsA/spermidine export protein MdtJ

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ DH1 2204804 2204923 120 270 [3] [0] 266 tqsA/mdtJ AI‑2 transporter TqsA/spermidine export protein MdtJ

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? DH1 = 22048033 (0.010)263 (0.530) 160/296 2.2 99.4% intergenic (‑238/‑174) tqsA/mdtJ AI‑2 transporter TqsA/spermidine export protein MdtJ
?DH1 2204924 = 0 (0.000)intergenic (‑359/‑53) tqsA/mdtJ AI‑2 transporter TqsA/spermidine export protein MdtJ

Only 100 of 270 total aligned reads displayed.

AGTGAAAATATTTTGAGTTAATTCTTAAGCTATGATACAAATCAGGCGTGTTCAACTACCGAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTTACCCATCTTCGTACCTCATCCACTATCTTGCTCGTAGGTGAGCAGCACTGGCGCAACGCGCTTTAGTCCTTTCTTCTGCCAAAGCTTGCGCTATGCGCGGGGCTTGACCACTTTACTTTGGTTTCGCTGAATTAAGCGAAAATTAAAA  >  DH1/2204653‑2204952
                                                                                                                                                      |                                                                                                                                                     
aGTGAAAATATTTTGAGTTAATTCTTAAGCTATGATACAAATCAGGCGTGTTCAACTACCGAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATT                                                                                                                                                       >  6:1727470‑M1/1‑151 (MQ=255)
   gAAAATATTTTGAGTTAATTCTTAAGCTATGATACAAATCAGGCGTGTTCAACTACCGAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTgg                                                                                                                                                     <  1:896912‑M1/150‑3 (MQ=255)
    aaaaTATTTTGAGTTAATTCTTAAGCTATGATACAAATCAGGCGTGTTCAACTACCGAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtt                                                                                                                                                   >  3:35058‑M1/1‑147 (MQ=255)
          ttttGAGTTAATTCTTAAGCTATGATACAAATCAGGCGTGTTCAACTACCGAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATCGAAAAATTGTTCAGGACGTTATTTTCTACATTgttttcgctt                                                                                                                                             >  2:1357321‑M1/1‑141 (MQ=255)
           tttGAGTTAATTCTTAAGCTATGATACAAATCAGGCGTGTTCAACTACCGAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctg                                                                                                                                             >  7:1725274‑M1/1‑140 (MQ=255)
           tttGAGTTAATTCTTAAGCTATGATACAAATCAGGCGTGTTCAACTACCGAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctg                                                                                                                                             >  2:1661585‑M1/1‑140 (MQ=255)
           tttGAGTTAATTCTTAAGCTATGATACAAATCAGGCGTGTTCAACTACCGAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctg                                                                                                                                             <  2:1187946‑M1/150‑11 (MQ=255)
            ttGAGTTAATTCTTAAGCTATGATACAAATCAGGCGTGTTCAACTACCGAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctga                                                                                                                                            >  5:1947469‑M1/1‑139 (MQ=255)
            ttGAGTTAATTCTTAAGCTATGATACAAATCAGGCGTGTTCAACTACCGAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctga                                                                                                                                            <  3:577280‑M1/150‑12 (MQ=255)
              gAGTTAATTCTTAAGCTATGATACAAATCAGGCGTGTTCAACTACCGAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaatt                                                                                                                                         <  7:368230‑M1/151‑15 (MQ=255)
                   aaTTCTTAAGCTATGATACAAATCAGGCGTGTTCATCTACCGAGGACAATTATCATCCGCGATGACGAGAAGCAACTCTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTGTACATTggtttcgctgaattaagcg                                                                                                                                    <  1:632811‑M1/151‑20 (MQ=255)
                      tCTTAAGCTATGATACAAATCAGGCGTGTTCAACTACCGAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaa                                                                                                                                  >  1:1483923‑M1/1‑129 (MQ=255)
                         tAAGCTATGATACAAATCAGGCGTGTTCAACTACCGAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaat                                                                                                                               <  1:1085118‑M1/150‑25 (MQ=255)
                         tAAGCTATGATACAAATCAGGCGTGTTCAACTACCGAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaa                                                                                                                                <  5:759155‑M1/149‑24 (MQ=255)
                          aaGCTATGATACAAATCAGGCGTGTTCAACTACCGAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaatt                                                                                                                              <  2:1335183‑M1/150‑26 (MQ=255)
                          aaGCTATGATACAAATCAGGCGTGTTCAACTACCGAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaatt                                                                                                                              >  4:1228305‑M1/1‑125 (MQ=255)
                          aaGCTATGATACAAATCAGGCGTGTTCAACTACCGAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaatt                                                                                                                              >  6:445508‑M1/1‑125 (MQ=255)
                           aGCTATGATACAAATCAGGCGTGTTCAACTACCGAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaatta                                                                                                                             >  5:1436857‑M1/1‑124 (MQ=255)
                            gCTATGATACAAATCAGGCGTGTTCAACTACCGAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaa                                                                                                                            >  8:1376317‑M1/1‑123 (MQ=255)
                               aTGATACAAATCAGGCGTGTTCAACTACCGAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaat                                                                                                                         <  6:1476856‑M1/150‑31 (MQ=255)
                               aTGATACAAATCAGGCGTGTTCAACTACCGAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaat                                                                                                                         <  3:12954‑M1/150‑31 (MQ=255)
                               aTGATACAAATCAGGCGTGTTCAACTACCGAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaa                                                                                                                          <  2:1512117‑M1/149‑30 (MQ=255)
                                 gATACAAATCAGGCGTGTTCAACTACCGAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataa                                                                                                                       <  1:484366‑M1/150‑33 (MQ=255)
                                  aTACAAATCAGGCGTGTTCAACTACCGAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataatt                                                                                                                     <  1:403791‑M1/151‑35 (MQ=255)
                                   tACAAATCAGGCGTGTTCAACTACCGAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaataaaaataatt                                                                                                                     >  6:331305‑M1/1‑116 (MQ=255)
                                    aCAAATCAGGCGTGTTCAACTACCGAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattataataattc                                                                                                                    <  4:159827‑M1/150‑36 (MQ=255)
                                     cAAATCAGGCGTGTTCAACTACCGAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctc                                                                                                                  >  3:595202‑M1/1‑114 (MQ=255)
                                        aTCAGGCGTGTTCAACTACCGAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttg                                                                                                               <  3:1660593‑M1/151‑41 (MQ=255)
                                        aTCAGGCGTGTTCAACTACCGAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttg                                                                                                               <  2:1721057‑M1/151‑41 (MQ=255)
                                         tCAGGCGTGTTCAACTACCGAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgc                                                                                                              >  5:860038‑M1/1‑110 (MQ=255)
                                              cGTGTTCAACTACCGAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaaataagcgaaaattaaaataattctcttgcagg                                                                                                           >  5:1155839‑M1/1‑105 (MQ=255)
                                               gtgtTCAACTACCGAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggaga                                                                                                        <  2:411249‑M1/151‑48 (MQ=255)
                                               gtgtTCAACTACCGAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcagg                                                                                                           >  2:1474529‑M1/1‑104 (MQ=255)
                                                  ttCAACTACCGAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaagg                                                                                                     >  1:1794918‑M1/1‑101 (MQ=255)
                                                   tCAACTACCGATGACAATTATCATCCGCGATGACGAGAATCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaagg                                                                                                     <  3:1127886‑M1/150‑51 (MQ=255)
                                                   tCAACTACCGAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaagg                                                                                                     <  4:1107935‑M1/150‑51 (MQ=255)
                                                    cAACTACCGAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggac                                                                                                   <  3:696650‑M1/151‑53 (MQ=255)
                                                     aaCTACCGAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggac                                                                                                   >  5:474456‑M1/1‑98 (MQ=255)
                                                      aCTACCGAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaa                                                                                                 <  5:1287940‑M1/151‑55 (MQ=255)
                                                      aCTACCGAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggaca                                                                                                  >  5:391309‑M1/1‑97 (MQ=255)
                                                      aCTACCGAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggaca                                                                                                  >  5:1463324‑M1/1‑97 (MQ=255)
                                                         aCCGAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagagaaaattaaaataattctcttgcagcagaaggacaatgt                                                                                              >  2:899911‑M1/1‑94 (MQ=255)
                                                           cGAGTACAATTATCATCCGCGATGTCGTGAATCAACACTGAGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTTCATTgctttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtat                                                                                            <  6:1542308‑M1/151‑60 (MQ=255)
                                                           cGAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtat                                                                                            <  8:30497‑M1/151‑60 (MQ=255)
                                                            gAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtata                                                                                           <  2:335908‑M1/151‑61 (MQ=255)
                                                            gAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtat                                                                                            >  5:1910439‑M1/1‑91 (MQ=255)
                                                              ggACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatt                                                                                         >  1:703018‑M1/1‑89 (MQ=255)
                                                               gACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatattt                                                                                        <  8:1652886‑M1/151‑64 (MQ=255)
                                                                  aaTTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttatt                                                                                     >  7:1826190‑M1/1‑85 (MQ=255)
                                                                   aTTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattg                                                                                    >  7:1465059‑M1/1‑84 (MQ=255)
                                                                     tATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattgg                                                                                   >  5:1534343‑M1/1‑82 (MQ=255)
                                                                      atcatcCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggat                                                                                 >  7:1174199‑M1/1‑81 (MQ=255)
                                                                           ccGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggatttta                                                                             <  4:1136002‑M1/150‑75 (MQ=255)
                                                                                  gACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtc                                                                      <  4:946811‑M1/150‑82 (MQ=255)
                                                                                   aCGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctg                                                                    >  7:1309109‑M1/1‑68 (MQ=255)
                                                                                      agaAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctggct                                                                 >  8:1207610‑M1/1‑65 (MQ=255)
                                                                                       gaAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctggcta                                                                <  1:116215‑M1/151‑88 (MQ=255)
                                                                                       gaAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctggct                                                                 >  3:812924‑M1/1‑64 (MQ=255)
                                                                                            aaCACTGCGGATAATTGTAATATTATTGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctggctattgct                                                           <  4:753654‑M1/151‑93 (MQ=255)
                                                                                            aaCACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctggctattgct                                                           >  1:1367242‑M1/1‑59 (MQ=255)
                                                                                            aaCACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctggctattgct                                                           <  7:697410‑M1/151‑93 (MQ=255)
                                                                                                  gCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctggctattgctacaga                                                      <  4:1002195‑M1/150‑98 (MQ=255)
                                                                                                    ggATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctggctattgctacagaaat                                                   >  7:39215‑M1/1‑51 (MQ=255)
                                                                                                    ggATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctggctattgctacagaaat                                                   <  1:253805‑M1/151‑101 (MQ=255)
                                                                                                      aTAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctggctattgctacagaaatta                                                 <  5:1673984‑M1/151‑103 (MQ=255)
                                                                                                      aTAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctggctattgctacagaaatt                                                  >  4:716800‑M1/1‑49 (MQ=255)
                                                                                                      aTAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtatggctattgctacagaaatt                                                  >  6:658388‑M1/1‑49 (MQ=255)
                                                                                                       tAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctggctattgctacagaaattac                                                >  3:1907010‑M1/1‑48 (MQ=255)
                                                                                                        aaTTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttagctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctggctattgctacagaaattacc                                               >  6:952589‑M1/1‑47 (MQ=255)
                                                                                                         aTTGTAATATTATGGACAATTTGTTCCGGACGTTATTTGCTACATTggtttcgctgaattaagcgaaaattacaataattctcttgcaggagacggacaatgtatatttattggattttattaggtctggctattgctacagaaattaccg                                              <  2:702477‑M1/151‑106 (MQ=255)
                                                                                                         aTTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctggctattgctacagaaattaccg                                              <  5:1794597‑M1/151‑106 (MQ=255)
                                                                                                         aTTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctggctattgctacagaaattaccg                                              <  2:570594‑M1/151‑106 (MQ=255)
                                                                                                         aTTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctggctattgctacagaaattaccg                                              <  3:892243‑M1/151‑106 (MQ=255)
                                                                                                           tGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctggctattgctacagaaattaccggt                                            >  3:861839‑M1/1‑44 (MQ=255)
                                                                                                           tGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctggctattgctacagaaattaccgg                                             <  8:266588‑M1/150‑107 (MQ=255)
                                                                                                           tGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctggctattgctacagaaattacaggt                                            >  6:605464‑M1/1‑44 (MQ=255)
                                                                                                           tGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctggctattgctaaagaaattaccggt                                            >  4:441185‑M1/1‑44 (MQ=255)
                                                                                                             tAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctggctattgctacagaaattaccggtac                                          >  6:1391089‑M1/1‑42 (MQ=255)
                                                                                                              aaTATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctggctattgctacagaaattaccggtac                                          <  7:262068‑M1/150‑110 (MQ=255)
                                                                                                               atatTATGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctggctattgctacagaaattaccggtacgc                                        <  8:1463129‑M1/151‑112 (MQ=255)
                                                                                                                 attatGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctggctattgctacagaaattaccggtacgctg                                      >  1:1624244‑M1/1‑38 (MQ=255)
                                                                                                                    atGGACAATTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctggctattgctacagaaattaccggtacgctgtc                                    >  2:1855459‑M1/1‑35 (MQ=255)
                                                                                                                          aaTTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctggctattgctacagaaattaccggtacgctgtcaatgaa                              >  4:1729832‑M1/1‑29 (MQ=255)
                                                                                                                           aTTTGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctggctattgctacagaaattaccggtacgctgtcaatgaaat                            >  3:12085‑M1/1‑28 (MQ=255)
                                                                                                                             ttGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctggctatttctacagaaattaccggtacgctgtcaatgaaatgg                          >  2:35096‑M1/1‑26 (MQ=255)
                                                                                                                             ttGTTCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctggctattgctacagaaattaccggtacgctgtcaatgaaatgg                          >  5:163423‑M1/1‑26 (MQ=255)
                                                                                                                                 tCAGGACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctggctattgctacagaaattaccggtacgctgtcaatgaaatgggcg                       >  7:1582358‑M1/1‑22 (MQ=255)
                                                                                                                                    ggACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctggctattgctacagaaattaccggtacgctgtcaatgaaatgggcgagcg                   >  8:1553481‑M1/1‑19 (MQ=255)
                                                                                                                                     gACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctggctattgctacagaaattaccggtacgctgtcaatgaaatgggcgagcgt                  >  6:233134‑M1/1‑18 (MQ=255)
                                                                                                                                     gACGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctggctattgctacagaaattaccggtacgctgtcaatgaaatgggcgagcgt                  >  3:1916547‑M1/1‑18 (MQ=255)
                                                                                                                                     cacGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctggctattgctacacaaattaccggtacgctgtcaatgaaatgggcgagcgt                  >  8:812622‑M1/2‑18 (MQ=255)
                                                                                                                                      aCGTTATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctggctattgctacagaaattaccggtacgctgtcaatgaaatgggcgagcgtc                 >  1:87259‑M1/1‑17 (MQ=255)
                                                                                                                                          tATTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctggctattgctacagaaattaccggtacgctgtcaatgaaatgggcgagcgtcagtg             >  5:769006‑M1/1‑13 (MQ=255)
                                                                                                                                           aTTTTCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctggctattgctacagaaattaccggtacgctgtcaatgaaatgggcgagcgtcagtg             >  5:1198641‑M1/1‑12 (MQ=255)
                                                                                                                                             tttCTACATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctggctattgctacagaaattaccggtacgctgtcaatgaaatgggcgagcgtcagtgag           <  8:539927‑M1/150‑141 (MQ=255)
                                                                                                                                                  aCATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctggctattgctacagaaattaccggtacgctgtcaatgaaatgggcgagcgtcagtgagggaaat     >  8:1299545‑M1/1‑5 (MQ=255)
                                                                                                                                                  aCATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctggctattgctacagaaattaccggtacgctgtcaatgaaatgggcgagcgtcagtgagggaaat     >  1:1906165‑M1/1‑5 (MQ=255)
                                                                                                                                                  aCATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctggctattgctacagaaattaccggtacgctgtcaatgaaatgggcgagcgtcagtgagggaa       <  8:1830485‑M1/149‑145 (MQ=255)
                                                                                                                                                   cATTggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctggctattgctacagaaattaccggtacgctgtcaatgaaatgggcgagcgtcagtgagggaaatg    <  1:1027273‑M1/151‑148 (MQ=255)
                                                                                                                                                      tggtttcgctgaattaagcgaaaattaaaataattctcttgcaggagaaggacaatgtatatttattggattttattaggtctggctattgctacagaaattaccggtacgctgtcaatgaaatgggcgagcgtcagtgagggaaatggc  <  4:315904‑M1/150‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
AGTGAAAATATTTTGAGTTAATTCTTAAGCTATGATACAAATCAGGCGTGTTCAACTACCGAGGACAATTATCATCCGCGATGACGAGAAGCAACACTGCGGATAATTGTAATATTATGGACAATTTGTTCAGGACGTTATTTTCTACATTTACCCATCTTCGTACCTCATCCACTATCTTGCTCGTAGGTGAGCAGCACTGGCGCAACGCGCTTTAGTCCTTTCTTCTGCCAAAGCTTGCGCTATGCGCGGGGCTTGACCACTTTACTTTGGTTTCGCTGAATTAAGCGAAAATTAAAA  >  DH1/2204653‑2204952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑